Protein Blotting Detection and Imaging

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Overview

Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (immunodetection) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.

Related Topics: Protein Blotting Methods, Membranes and Blotting Papers, Transfer Buffers, and Transfer Conditions.


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Protein Detection Systems

Protein Detection Systems

Protein detection systems and their sensitivity.

 

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Literature
Number Description Download
2895 Protein Blotting Guide, Ver C Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
Number Description Options
6216 Detection Buffer Formulations Click to download
6217 Total Protein Detection Click to download
6218 Blot Stripping & Reprobing Click to download
6219 Immunodetection Click to download
 
 
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Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (<a href="/evportal/destination/solutions?catID=LUSQ4HE8Z">immunodetection</a>) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.</p> <p><strong>Related Topics</strong>: <a href="/evportal/destination/solutions?catID=LUSPPSESH">Protein Blotting Methods</a>, <a href="/evportal/destination/solutions?catID=LUSPSJ8UU">Membranes and Blotting Papers</a>, <a href="/evportal/destination/solutions?catID=LUSQA88UU">Transfer Buffers</a>, and <a href="/evportal/destination/solutions?catID=LUSPTIMNI">Transfer Conditions</a>.</p> <h3 class="clear"><br /></h3> <div class="bannerAT"> <div class="bannerText ddpcrText" style="width: 390px !important;"> <h2 class="banner_header">Stain-Free Technology</h2> <p style="padding:0 !important;margin:10px 0 !important;">See how you can streamline your protein electrophoresis and western blotting experiments.</p> <a class="linkgeneration" href="/en-us/applications-technologies/stain-free-technology">Find out more &raquo;</a></div> <img src="/webroot/web/images/lsr/global/english/solutions/stain-free-technology.jpg" alt="Stain-Free Technology" width="615" height="120" /></div> <script src="/webroot/web/js/countrySpecific-min.js" type="text/javascript"></script> <script type="text/javascript"><!-- $(document).ready(function(){ setSterlingUrlsToHtmlHrefVariables(); $('a.linkgeneration').each(function(){ $(this).attr('href', $(this).attr('href').replace('_locale', languageCode + '-' + countryCode).replace('_verticalUrl', currentVerticalUrlTitle).replace('_defaultVerticalUrl', defaultVerticalUrlTitle).replace('_feedbackCMSID',feedbackCMSID)); }); }); // --></script> Protein Detection Systems <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/western_blotting/technology_detail/pet25_protein_detection_systems.gif" alt="Protein Detection Systems" width="530px" height="580px" /></p> <p class="caption"><strong>Protein detection systems and their sensitivity.</strong></p> <div class="top"><a href="#helptop">Back to Top</a></div> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" border="0" cellspacing="0" cellpadding="0"> <tbody> <tr> <th>Number</th> <th>Description</th> <th class="options">Options</th> </tr> <tr> <td width="100">6216</td> <td width="350">Detection Buffer Formulations</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6216.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6217</td> <td width="350">Total Protein Detection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6217.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6218</td> <td width="350">Blot Stripping &amp; Reprobing</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6218.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6219</td> <td width="350">Immunodetection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6219.pdf" target="_blank"><span>Click to download</span></a></td> </tr> </tbody> </table> 2895 2032 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot Turbo Transfer System ->MTS::LGOQBW15##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot SD Semi-Dry Transfer Cell ->MT::b92801aa-76b7-45c9-825e-672589d01b00##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Mini Trans-Blot Cell ->MT::589ca8f7-5751-487a-a453-571ee8cc8b7e##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Criterion Blotter ->MT::351faa8e-680e-4892-9778-c8c722e9b3b2##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Trans-Blot Cell ->MT::a9900af6-f596-45c8-859e-9985048a418e## Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B## Life Science Research/Solutions/Applications/Protein Separation and Analysis/Fast Separation Transfer and Analysis ->MTS::LUSOUMFCN## Eddie C Protein Blotting Detection and Imaging 12/08/11 11:12 AM 12/08/21 11:24 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Technologies/Western_Blotting N 0
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