Protein Blotting Detection and Imaging

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en-us LUSPULKSY Protein Blotting Detection and Imaging Protein Blotting Detection and Imaging /webroot/web/html/lsr/solutions/technologies/western_blotting <p>Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (<a href="/evportal/destination/solutions?catID=LUSQ4HE8Z">immunodetection</a>) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.</p> <p><strong>Related Topics</strong>: <a href="/evportal/destination/solutions?catID=LUSPPSESH">Protein Blotting Methods</a>, <a href="/evportal/destination/solutions?catID=LUSPSJ8UU">Membranes and Blotting Papers</a>, <a href="/evportal/destination/solutions?catID=LUSQA88UU">Transfer Buffers</a>, and <a href="/evportal/destination/solutions?catID=LUSPTIMNI">Transfer Conditions</a>.</p> <h3 class="clear"><br /></h3> <div class="bannerAT"> <div class="bannerText ddpcrText" style="width: 390px !important;"> <h2 class="banner_header">Stain-Free Technology</h2> <p style="padding:0 !important;margin:10px 0 !important;">See how you can streamline your protein electrophoresis and western blotting experiments.</p> <a class="linkgeneration" href="/en-us/applications-technologies/stain-free-technology">Find out more &raquo;</a></div> <img src="/webroot/web/images/lsr/global/english/solutions/stain-free-technology.jpg" alt="Stain-Free Technology" width="615" height="120" /></div> <script src="/webroot/web/js/countrySpecific-min.js" type="text/javascript"></script> <script type="text/javascript"><!-- $(document).ready(function(){ setSterlingUrlsToHtmlHrefVariables(); $('a.linkgeneration').each(function(){ $(this).attr('href', $(this).attr('href').replace('_locale', languageCode + '-' + countryCode).replace('_verticalUrl', currentVerticalUrlTitle).replace('_defaultVerticalUrl', defaultVerticalUrlTitle).replace('_feedbackCMSID',feedbackCMSID)); }); }); // --></script> Protein Detection Systems <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/western_blotting/technology_detail/pet25_protein_detection_systems.gif" alt="Protein Detection Systems" width="530px" height="580px" /></p> <p class="caption"><strong>Protein detection systems and their sensitivity.</strong></p> <div class="top"><a href="#helptop">Back to Top</a></div> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" border="0" cellspacing="0" cellpadding="0"> <tbody> <tr> <th>Number</th> <th>Description</th> <th class="options">Options</th> </tr> <tr> <td width="100">6216</td> <td width="350">Detection Buffer Formulations</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6216.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6217</td> <td width="350">Total Protein Detection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6217.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6218</td> <td width="350">Blot Stripping &amp; Reprobing</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6218.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6219</td> <td width="350">Immunodetection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6219.pdf" target="_blank"><span>Click to download</span></a></td> </tr> </tbody> </table> 2895 Protein Blotting Guide, Ver C 2895 /webroot/web/pdf/lsr/literature/Bulletin_2895.pdf Literature PDF Other /webroot/web/images/general/icons/icon_pdf.gif Protein Blotting Guide No Protein Blotting Guide, Ver C 2895 Bulletin 2895, horseradish peroxidase (HRP), enzyme-antibody complex, alkaline phosphatase (AP), nitroblue tetrazolium (NBT) tablets, BCIP, secondary antibody probe, amido black, streptavidin-biotin, anionic dye, total protein blotting stain, nitrocellulose, supported nitrocellulose or polyvinylidene difluoride (PVDF) or low fluorescence LF PVDF membranes, antibody, immunoglobulin (IgG), antigen, assay, avidin, biotin, background, non-specific noise, Bjerrum Schafer-Nielsen buffer, blocking reagent, Western blotting, BLOTTO, chemiluminescence, colloidal gold, color development reagent, colorimetric detection, chemiluminescent detection, enzyme-antibody conjugate, Coomassie Blue stain, diaminobenzidine (DAB), dot blot, Dunn buffer, electrophoretic blotting, foam pads, filter paper, gelatin, high-intensity transfer, immunoassay, immunoblotting, immunodetection, ligand, membrane, membrane/filter paper sandwiches, microfiltration blotting, multiplexing, multiscreen apparatus, native PAGE, polyacrylamide gel electrophoresis, NHS-biotin, non-enzymatic probe, nonenzymatic probe, non-fat dry milk, non-specific binding, phycobiliprotein, power supply, primary antibody, prestained standards, molecular weight markers or ladders, Protein A, Protein G, rapid semi-dry blotting, SDS-PAGE, sodium dodecyl sulfate, sodium dodecyl sulphate, signal-to-noise ratio, signal to noise ratio, Stain-free technology, CCD imager, charge-coupled device, StrepTactin, Strep-tag sequence, substrate, super cooling coil, tank blotting, Towbin buffer, Tween 20 2032 Western Blotting Detection Reagents Brochure, Rev F 2032 /webroot/web/pdf/lsr/literature/Bulletin_2032F.pdf Literature PDF Product_Information_Sheets /webroot/web/images/general/icons/icon_pdf.gif Western Blotting Detection Reagents Brochure No Western Blotting Detection Reagents Brochure, Rev F 2032 western, blotting, blot, protein, reagent, specific antigen, horseradish peroxidase, alkaline phosphatase, primary, secondary, antibody, conjugate, conjugates, signal, total protein, block nonspecific sites, incubate, fluorescent stain, anionic dye, colloidal gold, chemiluminescence, color development, selection guide, immune-star, westernc, chemiluminescent, hrp, ap, amplified opti-4cn, dab, immun-blot, enzyme-conjugated antibodies, substrate, luminal, cdp-star, nitrocellulose, pvdf membrane blots, ccd imager, quantity one software, 170-5040, 170-5041, 170-5043, 170-5042, 170-5044, 170-5045, 170-5047, 170-5046. 170-5010, 170-5011, 170-5012, 170-5018, 170-5070, 1705040, 1705041, 1705043, 1705042, 1705044, 1705045, 1705047, 1705046, 1705010, 1705011, 1705012, 1705018, 1705070, bio-safe coomassie blue, uv, sypro ruby, 170-6527, 170-3127, 161-0786, 1706527, 1703127, 1610786, colorimetric, film-based, film, bcip/nbt, bcip, nbt, 4cn, premixed, sensitivity, background, 170-6463, 170-6464, 170-6465, 170-6460, 170-6461, 170-6462, 170-8237, 170-8239, 170-8240, 170-8235, 170-8238, 170-6432, 170-6431, 170-6534, 170-6539, 170-6532, 170-6535, 170-6518, 170-6520, 170-6521, 170-6515, 170-6516, 172-1050, 170-6533, 170-6528, 170-3554, 170-6522, 170-6425, powdered, liquid, standards, precision plus protein, strep-tag, strep-tagged, streptactin, on-blot, molecular weight estimation, transfer efficiency, unstained, dual color, xtra, kaleidoscope, all blue, biotinylated, prestained sds-page, band pattern, monitoring, low range, high, broad, avidin, 161-0376, 161-0385, 161-0363, 161-0374, 161-0394, 161-0377, 161-0375, 161-0395, 161-0373, 161-0393, 161-0305, 161-0309, 161-0318, 161-0324, 161-0325, 161-0311, 161-0306, 161-0319, 161-0312, 161-0313, 161-0307, 161-0308, 161-0321, 161-0322, 161-0380, 161-0382, 1610376, 1610385, 1610363, 1610374, 1610394, 1610377, 1610375, 1610395, 1610373, 1610393, 1610305, 1610309, 1610318, 1610324, 1610325, 1610311, 1610306, 1610319, 1610312, 1610313, 1610307, 1610308, 1610321, 1610322, 1706528, 1706533, 1610380, 1610382, 170-5015, CDPstar, 1705015 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot Turbo Transfer System ->MTS::LGOQBW15##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot SD Semi-Dry Transfer Cell ->MT::b92801aa-76b7-45c9-825e-672589d01b00##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Mini Trans-Blot Cell ->MT::589ca8f7-5751-487a-a453-571ee8cc8b7e##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Criterion Blotter ->MT::351faa8e-680e-4892-9778-c8c722e9b3b2##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Trans-Blot Cell ->MT::a9900af6-f596-45c8-859e-9985048a418e## Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B## Life Science Research/Solutions/Applications/Protein Separation and Analysis/Fast Separation Transfer and Analysis ->MTS::LUSOUMFCN## Eddie C Protein Blotting Detection and Imaging 12/08/11 11:12 AM 12/08/21 11:24 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Western_Blotting N 0 Total Protein Detection /en-us/applications-technologies/applications-technologies/total-protein-detection?ID=LUSQ3K15 Introduction to Immunodetection /en-us/applications-technologies/applications-technologies/introduction-immunodetection?ID=LUSQ4HE8Z Imaging - Analysis and Documentation /en-us/applications-technologies/applications-technologies/imaging-analysis-documentation?ID=LUSQ8NC4S Introduction to Western Blotting /en-us/applications-technologies/applications-technologies/protein-blotting-detection-imaging?ID=LUSPPAKG4

Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (immunodetection) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.

Related Topics: Protein Blotting Methods, Membranes and Blotting Papers, Transfer Buffers, and Transfer Conditions.


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Protein Detection Systems

Protein Detection Systems

Protein detection systems and their sensitivity.

 

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Literature
Number Description Download
2895 Protein Blotting Guide, Ver C Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
Number Description Options
6216 Detection Buffer Formulations Click to download
6217 Total Protein Detection Click to download
6218 Blot Stripping & Reprobing Click to download
6219 Immunodetection Click to download
 
 
LUSPULKSY [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [54.224.11.137, 10.232.3.215] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/applications-technologies/protein-blotting-detection-imaging] [x-amzn-trace-id] = [Root=1-5b7577e2-b6ab87a2c7c4c024617c18c8] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSPULKSY] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/applications-technologies/protein-blotting-detection-imaging] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/protein-blotting-detection-imaging LSR LUSPULKSY Protein Blotting Detection and Imaging Protein Blotting Detection and Imaging /webroot/web/html/lsr/solutions/technologies/western_blotting <p>Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (<a href="/evportal/destination/solutions?catID=LUSQ4HE8Z">immunodetection</a>) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.</p> <p><strong>Related Topics</strong>: <a href="/evportal/destination/solutions?catID=LUSPPSESH">Protein Blotting Methods</a>, <a href="/evportal/destination/solutions?catID=LUSPSJ8UU">Membranes and Blotting Papers</a>, <a href="/evportal/destination/solutions?catID=LUSQA88UU">Transfer Buffers</a>, and <a href="/evportal/destination/solutions?catID=LUSPTIMNI">Transfer Conditions</a>.</p> <h3 class="clear"><br /></h3> <div class="bannerAT"> <div class="bannerText ddpcrText" style="width: 390px !important;"> <h2 class="banner_header">Stain-Free Technology</h2> <p style="padding:0 !important;margin:10px 0 !important;">See how you can streamline your protein electrophoresis and western blotting experiments.</p> <a class="linkgeneration" href="/en-us/applications-technologies/stain-free-technology">Find out more &raquo;</a></div> <img src="/webroot/web/images/lsr/global/english/solutions/stain-free-technology.jpg" alt="Stain-Free Technology" width="615" height="120" /></div> <script src="/webroot/web/js/countrySpecific-min.js" type="text/javascript"></script> <script type="text/javascript"><!-- $(document).ready(function(){ setSterlingUrlsToHtmlHrefVariables(); $('a.linkgeneration').each(function(){ $(this).attr('href', $(this).attr('href').replace('_locale', languageCode + '-' + countryCode).replace('_verticalUrl', currentVerticalUrlTitle).replace('_defaultVerticalUrl', defaultVerticalUrlTitle).replace('_feedbackCMSID',feedbackCMSID)); }); }); // --></script> Protein Detection Systems <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/western_blotting/technology_detail/pet25_protein_detection_systems.gif" alt="Protein Detection Systems" width="530px" height="580px" /></p> <p class="caption"><strong>Protein detection systems and their sensitivity.</strong></p> <div class="top"><a href="#helptop">Back to Top</a></div> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" border="0" cellspacing="0" cellpadding="0"> <tbody> <tr> <th>Number</th> <th>Description</th> <th class="options">Options</th> </tr> <tr> <td width="100">6216</td> <td width="350">Detection Buffer Formulations</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6216.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6217</td> <td width="350">Total Protein Detection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6217.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6218</td> <td width="350">Blot Stripping &amp; Reprobing</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6218.pdf" target="_blank"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6219</td> <td width="350">Immunodetection</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6219.pdf" target="_blank"><span>Click to download</span></a></td> </tr> </tbody> </table> 2895 2032 Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot Turbo Transfer System ->MTS::LGOQBW15##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Semi-Dry Blotting Systems/Trans-Blot SD Semi-Dry Transfer Cell ->MT::b92801aa-76b7-45c9-825e-672589d01b00##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Mini Trans-Blot Cell ->MT::589ca8f7-5751-487a-a453-571ee8cc8b7e##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Criterion Blotter ->MT::351faa8e-680e-4892-9778-c8c722e9b3b2##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Western Blotting/Wet/Tank Blotting Systems/Trans-Blot Cell ->MT::a9900af6-f596-45c8-859e-9985048a418e## Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B## Life Science Research/Solutions/Applications/Protein Separation and Analysis/Fast Separation Transfer and Analysis ->MTS::LUSOUMFCN## Eddie C Protein Blotting Detection and Imaging 12/08/11 11:12 AM 12/08/21 11:24 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Western_Blotting N 0
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