Genomics Learning Center

Join Us for In-Depth Technical Courses in Real-Time qPCR

Become a qPCR expert through our free 5-part webinar series.

Consult with an Specialist

Questions about Genomics Applications?

Contact a Specialist

The Genomics Learning Center is a knowledge hub where you can find both general information on a range of genomics technologies and advanced solutions for PCR, qPCR/RT-PCR, digital PCR, and next-generation sequencing (NGS). The field of genomics research has grown incredibly and is constantly evolving, with new technologies pushing the boundaries of the study of genes and gene function. Whether you are an experienced genomics researcher or new to the field, it is critical to stay informed about the latest applications and techniques so you can design your experiments with confidence and drive your research forward.

Identification of Genomic Targets

Next-generation sequencing (NGS) and advanced PCR methods have revolutionized genomics and made sequencing accessible to a wide range of research applications. New technologies continue to advance long-range, direct, and single-cell sequencing, while new library preparation and nucleic acid quantification solutions help optimize workflows. Topics here include standard and emerging NGS methodologies for sequencing DNA and RNA targets plus advanced digital PCR tools for a range of genomics applications.

Latest Methods for the Identification of Genomic Targets

 

Quantification of Genomic Targets

PCR technology remains vital to genomic workflows, with new high-performance digital PCR systems offering vast improvements in accuracy and reproducibility. Digital PCR systems like droplet digital PCR (ddPCR) allow ultrasensitive and absolute nucleic acid quantification. While real-time PCR (qPCR) is often used for target detection, these droplet digital PCR systems provide exceptional sensitivity in detection of low abundant targets or complex targets, allelic variants (SNPs), or subtle changes in target levels. Topics here include the application of digital PCR technology to gene expression analysis, CNV analysis, and single-cell studies.

Latest Methods for the Quantification of Genomic Targets

 

Understanding Gene Function

The ultimate goal of genomics is to understand the function of genes in the context of health and disease. This is typically achieved by mutating or omitting the gene in question and analyzing the downstream cellular effects. CRISPR technology has greatly expanded our tool kit for targeted gene editing and introduced opportunities for novel gene therapies. Advancements in NGS are also allowing scientists to explore more of the non-coding transcriptome and revealing the diverse roles of non-coding RNAs. This section will explore topics in functional genomics, including genome editing, non-coding RNAs, and RNA-Seq data analysis.

Latest Methods for Understanding Genomic Function

 

More on Gene Expression

Introducing the Real-Time qPCR Learning Center

The Real-Time qPCR Learning Center is an educational resource covering the key steps of the qPCR assay. From RNA isolation, to qPCR setup and analysis, it includes best practices and recommendations from experts on how to improve your qPCR technique.

Get Tips

Related Topics and Products

  • Genomics

    PCR (Polymerase Chain Reaction)

    Learn how PCR works, how to choose the right PCR instrument, how to design, optimize, analyze, and troubleshoot PCR assays.

  • Real-Time PCR (qPCR)

    Discover an array of solutions to optimize, execute, and troubleshoot MIQE-compliant real-time PCR experiments.

  • Digital PCR (ddPCR)

    Learn how breakthrough digital PCR technology is used in droplet digital PCR (ddPCR) to provide ultrasensitive and absolute nucleic acid quantitation.

  • SARS-CoV-2 / COVID-19 Assay and Research Solutions

    Bio-Rad provides a wide range of products for use in the support of COVID-19 diagnostic screening, confirmation of test results, surveillance, and therapeutic & vaccine research and development.

  • ddPCR Master Class Videos

    Become an expert in all things ddPCR. Our master class sessions allow you to learn from the best in the field. From instrument overviews to informational sessions highlighting the implications of ddPCR in the real world, Master Classes have it all.

  • Targeted Genome Editing

    Learn about the major systems for targeted genome editing, including CRISPR/Cas9, some key applications, and workflow options.

 
  • Traditional PCR Systems

    Traditional PCR Systems

    DNA amplification instruments range from personal thermal cyclers to the flexible 1000-series.

  • qPCR Detection Systems

    qPCR Detection Systems

    These systems deliver sensitive, reliable detection of both singleplex and multiplex real-time PCR reactions.

  • Digital PCR

    Digital PCR

    Bio-Rad's unique Droplet Digital PCR technology provides absolute quantification of nucleic acids for a wide range of applications including cancer mutation studies, HIV quantification, and environmental monitoring.

  • PCR Plastics Consumables PCR Plastic Consumables

    PCR Plastic Consumables

    A large selection of thin-wall PCR tubes, plates, seals, and accessories precisely manufactured for optimal fit and cycling performance in Bio-Rad thermal cyclers, real-time PCR systems, ddPCR systems, and all major competitor systems.

  • PCR & qPCR Reagents

    A wide range of reagents for reverse transcription, PCR, and real-time PCR, optimized to generate accurate and reproducible data.

  • PrimePCR PCR Primers, Assays & Arrays

    Next-Generation Sequencing

    To assist researchers in answering the most complex biological questions, Bio-Rad offers a number of novel products for using NGS library preparation to enable cutting edge technologies, such as whole transcriptome sequencing (WTS) and single-cell sequencing.

  • PrimePCR PCR Primers, Assays & Arrays

    PrimePCR PCR Primers, Assays & Arrays

    Experimentally validated PCR primer and probe assays for gene expression, copy number variation, and mutation detection analysis for real-time PCR and Droplet Digital PCR.

  • Droplet Digital PCR Assays

    Droplet Digital PCR Assays

    Bio-Rad offers a comprehensive portfolio of Digital PCR Assays and Kits for numerous applications including Mutation Detection, Copy Number Determination, Genome Edit Detection, Gene Expression, Expert Design Assays, Residual DNA Quantification and Library Quantification.

ECCMID 2022 Conference Poster

COVID-19 Variant Detection with RT-qPCR Poster

2022 European Congress of Clinical Microbiology & Infectious Diseases (ECCMID) Conference

Learn how UCSF used PrimePCR SARS-CoV-2 single and multiple mutation assays to detect 4 SARS-CoV-2 variant mutations in a single well, on samples with Cq<40.