Gravity Chromatography

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en-us LUSNFX8UU Gravity Chromatography Gravity Chromatography /webroot/web/html/lsr/solutions/technologies/chromatography <p>Gravity chromatography is appropriate for laboratory scale and involves minimal instrumentation. It relies primarily on hand-packed columns and is particularly well suited for single-step purifications like desalting and affinity applications. This section provides an overview of gravity chromatography and selection guides to help you build your own gravity chromatography setup.</p> <p><strong> Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSNA7ESH">Low Pressure Chromatography Systems</a> and <a href="/evportal/destination/solutions?catID=LUSNE3C4S">Medium Pressure Chromatography Systems</a>.</p> General Setup of Gravity Chromatography <div style="float: right; margin-left: 30px; margin-right: 80px; width: 95px;"><img src="/webroot/web/images/lsr/solutions/technologies/lab_scale_chromatography/chromatography/technology_detail/chromt18_img1.jpg" alt="" width="74" height="350" /><br /> <span class="caption"><strong>Typical gravity column.</strong></span></div> <p>As pictured below, a typical gravity column involves a buffer reservoir at the top of the column with flow controlled using a stopcock or a tubing pincher at the column outlet. There are a number of refinements available to assist with the general design:</p> <ul> <li>Graduated reservoirs for column packing and buffer delivery</li> <li>Flow adaptors to minimize head space above the gel bed and minimize disruption of the gel bed during sample loading</li> <li>Column bed supports/frits to retain media</li> <li>Integral water jacket for temperature control</li> <li>Stopcocks to control flow rate</li> <li>Luer-Lok end cap fittings for tubing and serial column connections</li> <li>Fraction collectors</li> </ul> <p class="clear">&nbsp;</p> <div class="top"><a href="#helptop">Back to Top</a></div> Advantages of Gravity Chromatography <ul> <li>Simplicity &mdash; a gravity column is easy to design, set up, and operate</li> <li>Minimal start up costs &mdash; costs are limited to the researchers' needs. No injectors, pumps, detectors, or fraction collectors are required</li> <li>Disposable &mdash; many disposable prepacked and empty polypropylene columns are available</li> </ul> <p>&nbsp;</p> <div class="top"><a href="#helptop">Back to Top</a></div> <p>&nbsp;</p> General Considerations <p>Gravity chromatography is:</p> <ul> <li>A manual process requiring constant monitoring</li> <li>Not a high resolution method. It is ideal for single step purifications like desalting and affinity</li> <li>Time consuming. No automated injectors, pumps, or detectors are used to control sample delivery, flow rate, or fraction analysis</li> </ul> <p>Reproducibility is an issue if care is not taken to normalize methods. Manual sample loading can be a challenge due to the disruption of the column bed both before and after sample loading. <a title="Connecting a Flow Adaptor" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/chromatography/category_overlay/global/vid_econopac_flow_adapter.html' );" href="javascript:void(0);">Use a flow adaptor</a> to minimize these effects.</p> <p><strong> Adjusting flow rate</strong></p> <ul> <li>With a gravity flow system, the buffer reservoir must be placed above the column to ensure flow</li> <li>The flow rate can be controlled manually using a stopcock or a simple tubing pincher at the column outlet</li> <li>It is highly recommended to measure the volume and flow rate by counting drops/unit time: <br />1 drop = 50 &micro;l. Normalized methods will lead to more reproducible separation methods</li> <li>Fraction collectors are available that can count drops and dispense fractions defined by drop number or volume</li> </ul> <p><strong> Sample loading</strong></p> <ul> <li>Manual sample loading is not recommended in size exclusion/gel filtration chromatography. It is critical that the column bed remains undisturbed or the resolution will be compromised</li> <li>Bind and elute techniques work well, however, additional column washes become necessary due to the inherent sample dilution impact with this technique</li> </ul> <p><strong> Elution and fraction collection</strong></p> <ul> <li>It is highly recommended that the user employ a timer during elution and fraction collection to avoid the possibility of the column running dry</li> </ul> <div class="top"><a href="#helptop">Back to Top</a></div> Selection Guides <p>Bio-Rad offers a complete line of pre-packed and empty columns for gravity chromatography.</p> <p><strong>Empty Column Selection Guide</strong></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td class="pd_tableheader" width="95">Column</td> <td class="pd_tableheader">Application Requirement</td> <td class="pd_tableheader">Pressure Range</td> <td class="pd_tableheader">Volume Range</td> <td class="pd_tableheader">Best Used With</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=80498fdf-95f4-4998-b37d-fa0109013c46">Bio-Scale</a>&trade; <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=80498fdf-95f4-4998-b37d-fa0109013c46">MT</a></td> <td>High-resolution column</td> <td>0&ndash;1,000 psi</td> <td>1&ndash;22 ml</td> <td>High-performance system</td> </tr> <tr> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=2cfb1c6e-32e8-4c72-b532-dd39013d707d">Econo-Column</a><sup>&reg;</sup></td> <td>General purpose glass column</td> <td>0&ndash;15 psi</td> <td>1&ndash;1,374 ml</td> <td>Low pressure or gravity</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=3aaf45bf-6d18-4e09-b553-7484bb8bd5e7">Econo-Pac</a><sup>&reg;</sup></td> <td>General purpose plastic column</td> <td>0&ndash;15 psi</td> <td>1&ndash;20 ml</td> <td>Low pressure or gravity</td> </tr> <tr> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=4f7ce1ea-7c12-44e6-b435-049b88180b09">Poly-Prep</a><sup>&reg;</sup></td> <td>Disposable plastic column</td> <td>0&ndash;15 psi</td> <td>2 ml</td> <td>Gravity</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/en/US/LSR/Category/58ee7212-1c1f-4cdc-b348-eb91a641eced/Bio-Spin_and_Micro_Bio-Spin_Size_Exclusion_Columns">Bio-Spin</a><sup>&reg;</sup></td> <td>Disposable plastic column</td> <td>&mdash;</td> <td>1.2 ml</td> <td>Swinging bucket centrifuge</td> </tr> <tr> <td><a href="http://www.bio-rad.com/evportal/en/US/LSR/Category/58ee7212-1c1f-4cdc-b348-eb91a641eced/Bio-Spin_and_Micro_Bio-Spin_Size_Exclusion_Columns">Micro Bio-Spin</a>&trade;</td> <td>Disposable plastic column</td> <td>&mdash;</td> <td>0.8 ml</td> <td>Standard micro-centrifuge</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=7e64443c-73c6-44ba-84f2-4353f1fada65">EasyPack</a>&trade;</td> <td>Process scale</td> <td>3 bar (glass) <br /> 8.7 bar (stainless steel and acrylic)</td> <td>0.320&ndash;3,800 L</td> <td>Process chromatography system</td> </tr> </tbody> </table> <p><img src="http://biorad-ads.com/solutions/webroot/web/images/lsr/solutions/technologies/lab_scale_chromatography/chromatography/technology_detail/chromt18_img3.png" alt="Prepacked and empty columns." width="580" height="325" /></p> <p class="caption"><strong>Prepacked and empty columns.</strong></p> <p><strong> Glass Econo-Column Columns Selection Guide</strong><br /> Choose the correct Econo-Column chromatography column by length and inner diameter The corresponding flow adapter is shown with the columns. A flow adapter is not needed for ID's of 0.5 or 0.7 cm. A dash ( &ndash; ) indicates that the column is not available in that configuration (L, ID, or package type).</p> <table class="pd_gridlines pd_table" border="0"> <tbody> <tr class="pd_centeredcell"> <td colspan="2">&nbsp;</td> <td class="pd_centeredcell" colspan="6"><strong>Inner Diameter (ID)</strong></td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">0.5 cm</td> <td class="pd_centeredcell">0.7 cm</td> <td bgcolor="#f2f2f2">1.0 cm</td> <td class="pd_centeredcell">1.5 cm</td> <td bgcolor="#f2f2f2">2.5 cm</td> <td class="pd_centeredcell">5.0 cm</td> </tr> <tr class="pd_centeredcell"> <td align="left"><strong>Length</strong></td> <td align="left"><strong>Type</strong></td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">5 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4506">737-4506</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4706">737-4706</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4006">737-4006</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4150">737-4150</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4250">737-4250</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0507">737-0507</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0707">737-0707</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1007">737-1007</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1507">737-1507</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2507">737-2507</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell" align="left">&nbsp;</td> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0014">738-0014</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">10 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4511">737-4511</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4711">737-4711</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4011">737-4011</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4151">737-4151</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4251">737-4251</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0512">737-0512</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0712">737-0712</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1012">737-1012</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1512">737-1512</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2512">737-2512</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 1</td> <td class="pd_centeredcell" bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td class="pd_centeredcell" bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td class="pd_centeredcell" bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5011">737-5011</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0014">738-0014</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="3" align="left">15 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4516">737-4516</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4716">737-4716</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4156">737-4156</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0517">737-0517</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0717">737-0717</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1517">737-1517</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">20 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4521">737-4521</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4721">737-4721</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4021">737-4021</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4152">737-4152</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4252">737-4252</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0522">737-0522</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0722">737-0722</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1022">737-1022</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1522">737-1522</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2522">737-2522</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td height="19" align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5021">737-5021</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">30 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4731">737-4731</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4031">737-4031</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4153">737-4153</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4253">737-4253</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0732">737-0732</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1032">737-1032</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1532">737-1532</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2532">737-2532</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5031">737-5031</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td colspan="2">&nbsp;</td> <td class="pd_centeredcell" colspan="6"><strong>Inner Diameter (ID)</strong></td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">0.5 cm</td> <td class="pd_centeredcell">0.7 cm</td> <td bgcolor="#f2f2f2">1.0 cm</td> <td class="pd_centeredcell">1.5 cm</td> <td bgcolor="#f2f2f2">2.5 cm</td> <td class="pd_centeredcell">5.0 cm</td> </tr> <tr class="pd_centeredcell"> <td align="left"><strong>Length</strong></td> <td align="left"><strong>Type</strong></td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">50 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4751">737-4751</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4051">737-4051</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4155">737-4155</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0752">737-0752</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1052">737-1052</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1552">737-1552</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2551">737-2551</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5051">737-5051</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">70 cm</td> <td align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5071">737-5071</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">75 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1576">737-1576</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2576">737-2576</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">100 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1091">737-1091</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1591">737-1591</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2591">737-2591</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><span class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></span></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">120 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1093">737-1093</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1593">737-1593</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2593">737-2593</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><span class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></span></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td>&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left" valign="top">170 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1598">737-1598</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td>&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><span class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></span></td> <td bgcolor="#f2f2f2">&ndash;</td> <td>&ndash;</td> </tr> </tbody> </table> <p><strong> Jacketed Econo-Column Chromatography Columns Selection Guide</strong><br /> Choose the correct jacketed glass Econo-Column chromatography column by length and inner diameter. The corresponding flow adapter is shown with the columns. A dash ( &ndash; ) indicates that the column is not available in that configuration (L, ID, or package type).</p> <table class="pd_gridlines pd_table" border="0"> <tbody> <tr class="pd_centeredcell"> <td colspan="2">&nbsp;</td> <td class="pd_centeredcell" colspan="3"><strong>Inner Diameter (ID)</strong></td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">0.7 cm</td> <td bgcolor="#f2f2f2">1.0 cm</td> <td class="pd_centeredcell">1.5 cm</td> </tr> <tr class="pd_centeredcell"> <td align="left"><strong>Length</strong></td> <td align="left"><strong>Type</strong></td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">15 cm</td> <td align="left">Pkg of 1</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6108">737-6108</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6116">737-6116</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">30 cm</td> <td align="left">Pkg of 1</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6131">737-6131</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">50 cm</td> <td align="left">Pkg of 1</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6151">737-6151</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> </tr> </tbody> </table> <div class="top"><a href="#helptop">Back to Top</a></div> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="Connecting a Flow Adaptor" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/chromatography/category_overlay/global/vid_econopac_flow_adapter.html' );" href="javascript:void(0);"> <img style="border:none" src="http://www.bio-rad.com/webroot/web/images/lsr/products/chromatography/category_overlay_icons/global/econopac_flow_adaptor.jpg" alt="" /></a></div> <div class="videoDesc"><a title="Connecting a Flow Adaptor" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/chromatography/category_overlay/global/vid_econopac_flow_adapter.html' );" href="javascript:void(0);">Connecting a Flow Adaptor</a><br />This tutorial shows you how to attach a flow adpator to an Econo-Column chromatography column.</div> <div class="clear">&nbsp;</div> </div> Life Science Research/Products/Chromatography/Chromatography Columns/Low Pressure Chromatography Columns and Cartridges/Glass Econo-Columns ->MT::2cfb1c6e-32e8-4c72-b532-dd39013d707d##Life Science Research/Products/Chromatography/Chromatography Columns/Low Pressure Chromatography Columns and Cartridges/Glass Econo-Column Accessories ->MT::1f263c3d-607f-4e94-9603-b7233f057683##Life Science Research/Products/Chromatography/Chromatography Columns/Gravity and Spin Chromatography Columns/Empty Gravity Flow Columns/Econo-Pac Chromatography Columns ->MT::3aaf45bf-6d18-4e09-b553-7484bb8bd5e7##Life Science Research/Products/Chromatography/Chromatography Columns/Medium-Pressure Chromatography Columns/UNO Monolith Cation Exchange Columns ->MT::5c6ccb9e-6463-4dc8-8c03-ed1210efdde9##Process Separations/Products/Chromatography Media Sampling/Prepacked Bio-Scale Mini Protein A Cartridges/Bio-Scale Mini UNOsphere SUPrA Affinity Cartridges ->MT::86e7b29e-5914-4f73-9a9c-be81f51fed8c## Life Science Research/Solutions/Technologies/Protein Electrophoresis/Protein Sample Preparation ->MTS::LUSP108WI##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B##Life Science Research/Solutions/Technologies/Western Blotting ->MTS::LUSPPAKG4##Life Science Research/Solutions/Technologies/Imaging and Analysis ->MTS::LUSQC6MNI##Life Science Research/Solutions/Technologies/Surface Plasmon Resonance ->MTS::LUSM664EH## Karen Moss 12/30/11 03:16 PM 12/30/21 03:27 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Chromatography N 0 Introduction to Chromatography /en-us/applications-technologies/applications-technologies/gravity-chromatography?ID=LUSMIS7OP

Gravity chromatography is appropriate for laboratory scale and involves minimal instrumentation. It relies primarily on hand-packed columns and is particularly well suited for single-step purifications like desalting and affinity applications. This section provides an overview of gravity chromatography and selection guides to help you build your own gravity chromatography setup.

Related Topics: Low Pressure Chromatography Systems and Medium Pressure Chromatography Systems.

 

General Setup of Gravity Chromatography


Typical gravity column.

As pictured below, a typical gravity column involves a buffer reservoir at the top of the column with flow controlled using a stopcock or a tubing pincher at the column outlet. There are a number of refinements available to assist with the general design:

  • Graduated reservoirs for column packing and buffer delivery
  • Flow adaptors to minimize head space above the gel bed and minimize disruption of the gel bed during sample loading
  • Column bed supports/frits to retain media
  • Integral water jacket for temperature control
  • Stopcocks to control flow rate
  • Luer-Lok end cap fittings for tubing and serial column connections
  • Fraction collectors

 

 

Advantages of Gravity Chromatography

  • Simplicity — a gravity column is easy to design, set up, and operate
  • Minimal start up costs — costs are limited to the researchers' needs. No injectors, pumps, detectors, or fraction collectors are required
  • Disposable — many disposable prepacked and empty polypropylene columns are available

 

 

 

General Considerations

Gravity chromatography is:

  • A manual process requiring constant monitoring
  • Not a high resolution method. It is ideal for single step purifications like desalting and affinity
  • Time consuming. No automated injectors, pumps, or detectors are used to control sample delivery, flow rate, or fraction analysis

Reproducibility is an issue if care is not taken to normalize methods. Manual sample loading can be a challenge due to the disruption of the column bed both before and after sample loading. Use a flow adaptor to minimize these effects.

Adjusting flow rate

  • With a gravity flow system, the buffer reservoir must be placed above the column to ensure flow
  • The flow rate can be controlled manually using a stopcock or a simple tubing pincher at the column outlet
  • It is highly recommended to measure the volume and flow rate by counting drops/unit time:
    1 drop = 50 µl. Normalized methods will lead to more reproducible separation methods
  • Fraction collectors are available that can count drops and dispense fractions defined by drop number or volume

Sample loading

  • Manual sample loading is not recommended in size exclusion/gel filtration chromatography. It is critical that the column bed remains undisturbed or the resolution will be compromised
  • Bind and elute techniques work well, however, additional column washes become necessary due to the inherent sample dilution impact with this technique

Elution and fraction collection

  • It is highly recommended that the user employ a timer during elution and fraction collection to avoid the possibility of the column running dry
 

Selection Guides

Bio-Rad offers a complete line of pre-packed and empty columns for gravity chromatography.

Empty Column Selection Guide

Column Application Requirement Pressure Range Volume Range Best Used With
Bio-ScaleMT High-resolution column 0–1,000 psi 1–22 ml High-performance system
Econo-Column® General purpose glass column 0–15 psi 1–1,374 ml Low pressure or gravity
Econo-Pac® General purpose plastic column 0–15 psi 1–20 ml Low pressure or gravity
Poly-Prep® Disposable plastic column 0–15 psi 2 ml Gravity
Bio-Spin® Disposable plastic column 1.2 ml Swinging bucket centrifuge
Micro Bio-Spin Disposable plastic column 0.8 ml Standard micro-centrifuge
EasyPack Process scale 3 bar (glass)
8.7 bar (stainless steel and acrylic)
0.320–3,800 L Process chromatography system

Prepacked and empty columns.

Prepacked and empty columns.

Glass Econo-Column Columns Selection Guide
Choose the correct Econo-Column chromatography column by length and inner diameter The corresponding flow adapter is shown with the columns. A flow adapter is not needed for ID's of 0.5 or 0.7 cm. A dash ( – ) indicates that the column is not available in that configuration (L, ID, or package type).

  Inner Diameter (ID)
    0.5 cm 0.7 cm 1.0 cm 1.5 cm 2.5 cm 5.0 cm
Length Type            
5 cm Pkg of 4 737-4506 737-4706 737-4006 737-4150 737-4250
Pkg of 2 737-0507 737-0707 737-1007 737-1507 737-2507
  Flow adapter 738-0014 738-0016 738-0017
10 cm Pkg of 4 737-4511 737-4711 737-4011 737-4151 737-4251
Pkg of 2 737-0512 737-0712 737-1012 737-1512 737-2512
Pkg of 1 737-5011
Flow adapter 738-0014 738-0016 738-0017 738-0018
15 cm Pkg of 4 737-4516 737-4716 737-4156
Pkg of 2 737-0517 737-0717 737-1517
Flow adapter 738-0016
20 cm Pkg of 4 737-4521 737-4721 737-4021 737-4152 737-4252
Pkg of 2 737-0522 737-0722 737-1022 737-1522 737-2522
Pkg of 1 737-5021
Flow adapter 738-0015 738-0016 738-0017 738-0018
30 cm Pkg of 4 737-4731 737-4031 737-4153 737-4253
Pkg of 2 737-0732 737-1032 737-1532 737-2532
Pkg of 1 737-5031
Flow adapter 738-0015 738-0016 738-0017 738-0018
  Inner Diameter (ID)
    0.5 cm 0.7 cm 1.0 cm 1.5 cm 2.5 cm 5.0 cm
Length Type            
50 cm Pkg of 4 737-4751 737-4051 737-4155
Pkg of 2 737-0752 737-1052 737-1552 737-2551
Pkg of 1 737-5051
Flow adapter 738-0015 738-0016 738-0017 738-0018
70 cm Pkg of 1 737-5071
Flow adapter 738-0018
75 cm Pkg of 2 737-1576 737-2576
Flow adapter 738-0016 738-0017
100 cm Pkg of 2 737-1091 737-1591 737-2591
Flow adapter 738-0015 738-0016 738-0017
120 cm Pkg of 2 737-1093 737-1593 737-2593
Flow adapter 738-0015 738-0016 738-0017
170 cm Pkg of 2 737-1598
Flow adapter 738-0016

Jacketed Econo-Column Chromatography Columns Selection Guide
Choose the correct jacketed glass Econo-Column chromatography column by length and inner diameter. The corresponding flow adapter is shown with the columns. A dash ( – ) indicates that the column is not available in that configuration (L, ID, or package type).

  Inner Diameter (ID)
    0.7 cm 1.0 cm 1.5 cm
Length Type      
15 cm Pkg of 1 737-6108 737-6116
Flow adapter 738-0015
30 cm Pkg of 1 737-6131
Flow adapter 738-0015
50 cm Pkg of 1 737-6151
Flow adapter 738-0016
 

Related Content

 
Connecting a Flow Adaptor
This tutorial shows you how to attach a flow adpator to an Econo-Column chromatography column.
 
 
 
LUSNFX8UU [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [54.221.48.198, 10.232.18.83] [accept] = [*/*] [seourl] = [/en-us/applications-technologies/applications-technologies/gravity-chromatography] [x-amzn-trace-id] = [Root=1-5b4d8b4b-3b220c28ee9c7898b345e250] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSNFX8UU] [host] = [10.232.0.21:1776] [x-request-uri] = [/en-us/applications-technologies/applications-technologies/gravity-chromatography] [connection] = [Keep-Alive] [accept-encoding] = [gzip, deflate] [user-agent] = [rogerbot/1.2 (https://moz.com/help/guides/moz-procedures/what-is-rogerbot, rogerbot-crawler+aardwolf-production-crawler-39@moz.com)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/applications-technologies/gravity-chromatography LSR LUSNFX8UU Gravity Chromatography Gravity Chromatography /webroot/web/html/lsr/solutions/technologies/chromatography <p>Gravity chromatography is appropriate for laboratory scale and involves minimal instrumentation. It relies primarily on hand-packed columns and is particularly well suited for single-step purifications like desalting and affinity applications. This section provides an overview of gravity chromatography and selection guides to help you build your own gravity chromatography setup.</p> <p><strong> Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSNA7ESH">Low Pressure Chromatography Systems</a> and <a href="/evportal/destination/solutions?catID=LUSNE3C4S">Medium Pressure Chromatography Systems</a>.</p> General Setup of Gravity Chromatography <div style="float: right; margin-left: 30px; margin-right: 80px; width: 95px;"><img src="/webroot/web/images/lsr/solutions/technologies/lab_scale_chromatography/chromatography/technology_detail/chromt18_img1.jpg" alt="" width="74" height="350" /><br /> <span class="caption"><strong>Typical gravity column.</strong></span></div> <p>As pictured below, a typical gravity column involves a buffer reservoir at the top of the column with flow controlled using a stopcock or a tubing pincher at the column outlet. There are a number of refinements available to assist with the general design:</p> <ul> <li>Graduated reservoirs for column packing and buffer delivery</li> <li>Flow adaptors to minimize head space above the gel bed and minimize disruption of the gel bed during sample loading</li> <li>Column bed supports/frits to retain media</li> <li>Integral water jacket for temperature control</li> <li>Stopcocks to control flow rate</li> <li>Luer-Lok end cap fittings for tubing and serial column connections</li> <li>Fraction collectors</li> </ul> <p class="clear">&nbsp;</p> <div class="top"><a href="#helptop">Back to Top</a></div> Advantages of Gravity Chromatography <ul> <li>Simplicity &mdash; a gravity column is easy to design, set up, and operate</li> <li>Minimal start up costs &mdash; costs are limited to the researchers' needs. No injectors, pumps, detectors, or fraction collectors are required</li> <li>Disposable &mdash; many disposable prepacked and empty polypropylene columns are available</li> </ul> <p>&nbsp;</p> <div class="top"><a href="#helptop">Back to Top</a></div> <p>&nbsp;</p> General Considerations <p>Gravity chromatography is:</p> <ul> <li>A manual process requiring constant monitoring</li> <li>Not a high resolution method. It is ideal for single step purifications like desalting and affinity</li> <li>Time consuming. No automated injectors, pumps, or detectors are used to control sample delivery, flow rate, or fraction analysis</li> </ul> <p>Reproducibility is an issue if care is not taken to normalize methods. Manual sample loading can be a challenge due to the disruption of the column bed both before and after sample loading. <a title="Connecting a Flow Adaptor" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/chromatography/category_overlay/global/vid_econopac_flow_adapter.html' );" href="javascript:void(0);">Use a flow adaptor</a> to minimize these effects.</p> <p><strong> Adjusting flow rate</strong></p> <ul> <li>With a gravity flow system, the buffer reservoir must be placed above the column to ensure flow</li> <li>The flow rate can be controlled manually using a stopcock or a simple tubing pincher at the column outlet</li> <li>It is highly recommended to measure the volume and flow rate by counting drops/unit time: <br />1 drop = 50 &micro;l. Normalized methods will lead to more reproducible separation methods</li> <li>Fraction collectors are available that can count drops and dispense fractions defined by drop number or volume</li> </ul> <p><strong> Sample loading</strong></p> <ul> <li>Manual sample loading is not recommended in size exclusion/gel filtration chromatography. It is critical that the column bed remains undisturbed or the resolution will be compromised</li> <li>Bind and elute techniques work well, however, additional column washes become necessary due to the inherent sample dilution impact with this technique</li> </ul> <p><strong> Elution and fraction collection</strong></p> <ul> <li>It is highly recommended that the user employ a timer during elution and fraction collection to avoid the possibility of the column running dry</li> </ul> <div class="top"><a href="#helptop">Back to Top</a></div> Selection Guides <p>Bio-Rad offers a complete line of pre-packed and empty columns for gravity chromatography.</p> <p><strong>Empty Column Selection Guide</strong></p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td class="pd_tableheader" width="95">Column</td> <td class="pd_tableheader">Application Requirement</td> <td class="pd_tableheader">Pressure Range</td> <td class="pd_tableheader">Volume Range</td> <td class="pd_tableheader">Best Used With</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=80498fdf-95f4-4998-b37d-fa0109013c46">Bio-Scale</a>&trade; <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=80498fdf-95f4-4998-b37d-fa0109013c46">MT</a></td> <td>High-resolution column</td> <td>0&ndash;1,000 psi</td> <td>1&ndash;22 ml</td> <td>High-performance system</td> </tr> <tr> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=2cfb1c6e-32e8-4c72-b532-dd39013d707d">Econo-Column</a><sup>&reg;</sup></td> <td>General purpose glass column</td> <td>0&ndash;15 psi</td> <td>1&ndash;1,374 ml</td> <td>Low pressure or gravity</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=3aaf45bf-6d18-4e09-b553-7484bb8bd5e7">Econo-Pac</a><sup>&reg;</sup></td> <td>General purpose plastic column</td> <td>0&ndash;15 psi</td> <td>1&ndash;20 ml</td> <td>Low pressure or gravity</td> </tr> <tr> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=4f7ce1ea-7c12-44e6-b435-049b88180b09">Poly-Prep</a><sup>&reg;</sup></td> <td>Disposable plastic column</td> <td>0&ndash;15 psi</td> <td>2 ml</td> <td>Gravity</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/en/US/LSR/Category/58ee7212-1c1f-4cdc-b348-eb91a641eced/Bio-Spin_and_Micro_Bio-Spin_Size_Exclusion_Columns">Bio-Spin</a><sup>&reg;</sup></td> <td>Disposable plastic column</td> <td>&mdash;</td> <td>1.2 ml</td> <td>Swinging bucket centrifuge</td> </tr> <tr> <td><a href="http://www.bio-rad.com/evportal/en/US/LSR/Category/58ee7212-1c1f-4cdc-b348-eb91a641eced/Bio-Spin_and_Micro_Bio-Spin_Size_Exclusion_Columns">Micro Bio-Spin</a>&trade;</td> <td>Disposable plastic column</td> <td>&mdash;</td> <td>0.8 ml</td> <td>Standard micro-centrifuge</td> </tr> <tr class="pd_colorbackground"> <td><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=7e64443c-73c6-44ba-84f2-4353f1fada65">EasyPack</a>&trade;</td> <td>Process scale</td> <td>3 bar (glass) <br /> 8.7 bar (stainless steel and acrylic)</td> <td>0.320&ndash;3,800 L</td> <td>Process chromatography system</td> </tr> </tbody> </table> <p><img src="http://biorad-ads.com/solutions/webroot/web/images/lsr/solutions/technologies/lab_scale_chromatography/chromatography/technology_detail/chromt18_img3.png" alt="Prepacked and empty columns." width="580" height="325" /></p> <p class="caption"><strong>Prepacked and empty columns.</strong></p> <p><strong> Glass Econo-Column Columns Selection Guide</strong><br /> Choose the correct Econo-Column chromatography column by length and inner diameter The corresponding flow adapter is shown with the columns. A flow adapter is not needed for ID's of 0.5 or 0.7 cm. A dash ( &ndash; ) indicates that the column is not available in that configuration (L, ID, or package type).</p> <table class="pd_gridlines pd_table" border="0"> <tbody> <tr class="pd_centeredcell"> <td colspan="2">&nbsp;</td> <td class="pd_centeredcell" colspan="6"><strong>Inner Diameter (ID)</strong></td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">0.5 cm</td> <td class="pd_centeredcell">0.7 cm</td> <td bgcolor="#f2f2f2">1.0 cm</td> <td class="pd_centeredcell">1.5 cm</td> <td bgcolor="#f2f2f2">2.5 cm</td> <td class="pd_centeredcell">5.0 cm</td> </tr> <tr class="pd_centeredcell"> <td align="left"><strong>Length</strong></td> <td align="left"><strong>Type</strong></td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">5 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4506">737-4506</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4706">737-4706</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4006">737-4006</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4150">737-4150</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4250">737-4250</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0507">737-0507</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0707">737-0707</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1007">737-1007</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1507">737-1507</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2507">737-2507</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell" align="left">&nbsp;</td> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0014">738-0014</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">10 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4511">737-4511</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4711">737-4711</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4011">737-4011</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4151">737-4151</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4251">737-4251</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0512">737-0512</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0712">737-0712</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1012">737-1012</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1512">737-1512</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2512">737-2512</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 1</td> <td class="pd_centeredcell" bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td class="pd_centeredcell" bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td class="pd_centeredcell" bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5011">737-5011</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0014">738-0014</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="3" align="left">15 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4516">737-4516</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4716">737-4716</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4156">737-4156</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0517">737-0517</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0717">737-0717</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1517">737-1517</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">20 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4521">737-4521</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4721">737-4721</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4021">737-4021</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4152">737-4152</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4252">737-4252</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0522">737-0522</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0722">737-0722</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1022">737-1022</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1522">737-1522</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2522">737-2522</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td height="19" align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5021">737-5021</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">30 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4731">737-4731</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4031">737-4031</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4153">737-4153</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4253">737-4253</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0732">737-0732</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1032">737-1032</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1532">737-1532</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2532">737-2532</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5031">737-5031</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td colspan="2">&nbsp;</td> <td class="pd_centeredcell" colspan="6"><strong>Inner Diameter (ID)</strong></td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">0.5 cm</td> <td class="pd_centeredcell">0.7 cm</td> <td bgcolor="#f2f2f2">1.0 cm</td> <td class="pd_centeredcell">1.5 cm</td> <td bgcolor="#f2f2f2">2.5 cm</td> <td class="pd_centeredcell">5.0 cm</td> </tr> <tr class="pd_centeredcell"> <td align="left"><strong>Length</strong></td> <td align="left"><strong>Type</strong></td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="4" align="left">50 cm</td> <td align="left">Pkg of 4</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4751">737-4751</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4051">737-4051</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-4155">737-4155</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-0752">737-0752</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1052">737-1052</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1552">737-1552</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2551">737-2551</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5051">737-5051</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">70 cm</td> <td align="left">Pkg of 1</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-5071">737-5071</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0018">738-0018</a></td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">75 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1576">737-1576</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2576">737-2576</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">100 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1091">737-1091</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1591">737-1591</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2591">737-2591</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><span class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></span></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">120 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1093">737-1093</a></td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1593">737-1593</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-2593">737-2593</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell"><span class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></span></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0017">738-0017</a></td> <td>&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left" valign="top">170 cm</td> <td align="left">Pkg of 2</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-1598">737-1598</a></td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td bgcolor="#f2f2f2">&ndash;</td> <td>&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><span class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></span></td> <td bgcolor="#f2f2f2">&ndash;</td> <td>&ndash;</td> </tr> </tbody> </table> <p><strong> Jacketed Econo-Column Chromatography Columns Selection Guide</strong><br /> Choose the correct jacketed glass Econo-Column chromatography column by length and inner diameter. The corresponding flow adapter is shown with the columns. A dash ( &ndash; ) indicates that the column is not available in that configuration (L, ID, or package type).</p> <table class="pd_gridlines pd_table" border="0"> <tbody> <tr class="pd_centeredcell"> <td colspan="2">&nbsp;</td> <td class="pd_centeredcell" colspan="3"><strong>Inner Diameter (ID)</strong></td> </tr> <tr class="pd_centeredcell"> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> <td class="pd_centeredcell">0.7 cm</td> <td bgcolor="#f2f2f2">1.0 cm</td> <td class="pd_centeredcell">1.5 cm</td> </tr> <tr class="pd_centeredcell"> <td align="left"><strong>Length</strong></td> <td align="left"><strong>Type</strong></td> <td class="pd_centeredcell">&nbsp;</td> <td bgcolor="#f2f2f2">&nbsp;</td> <td class="pd_centeredcell">&nbsp;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">15 cm</td> <td align="left">Pkg of 1</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6108">737-6108</a></td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6116">737-6116</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">30 cm</td> <td align="left">Pkg of 1</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6131">737-6131</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0015">738-0015</a></td> <td class="pd_centeredcell">&ndash;</td> </tr> <tr class="pd_centeredcell"> <td rowspan="2" align="left">50 cm</td> <td align="left">Pkg of 1</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=737-6151">737-6151</a></td> </tr> <tr class="pd_centeredcell"> <td align="left">Flow adapter</td> <td class="pd_centeredcell">&ndash;</td> <td bgcolor="#f2f2f2">&ndash;</td> <td class="pd_centeredcell"><a href="http://www.bio-rad.com/evportal/destination/commerce/sku_detail?productID=738-0016">738-0016</a></td> </tr> </tbody> </table> <div class="top"><a href="#helptop">Back to Top</a></div> <div class="videowrap vwrap_last"> <div class="videoImg"><a title="Connecting a Flow Adaptor" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/chromatography/category_overlay/global/vid_econopac_flow_adapter.html' );" href="javascript:void(0);"> <img style="border:none" src="http://www.bio-rad.com/webroot/web/images/lsr/products/chromatography/category_overlay_icons/global/econopac_flow_adaptor.jpg" alt="" /></a></div> <div class="videoDesc"><a title="Connecting a Flow Adaptor" onclick="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/chromatography/category_overlay/global/vid_econopac_flow_adapter.html' );" href="javascript:void(0);">Connecting a Flow Adaptor</a><br />This tutorial shows you how to attach a flow adpator to an Econo-Column chromatography column.</div> <div class="clear">&nbsp;</div> </div> Life Science Research/Products/Chromatography/Chromatography Columns/Low Pressure Chromatography Columns and Cartridges/Glass Econo-Columns ->MT::2cfb1c6e-32e8-4c72-b532-dd39013d707d##Life Science Research/Products/Chromatography/Chromatography Columns/Low Pressure Chromatography Columns and Cartridges/Glass Econo-Column Accessories ->MT::1f263c3d-607f-4e94-9603-b7233f057683##Life Science Research/Products/Chromatography/Chromatography Columns/Gravity and Spin Chromatography Columns/Empty Gravity Flow Columns/Econo-Pac Chromatography Columns ->MT::3aaf45bf-6d18-4e09-b553-7484bb8bd5e7##Life Science Research/Products/Chromatography/Chromatography Columns/Medium-Pressure Chromatography Columns/UNO Monolith Cation Exchange Columns ->MT::5c6ccb9e-6463-4dc8-8c03-ed1210efdde9##Process Separations/Products/Chromatography Media Sampling/Prepacked Bio-Scale Mini Protein A Cartridges/Bio-Scale Mini UNOsphere SUPrA Affinity Cartridges ->MT::86e7b29e-5914-4f73-9a9c-be81f51fed8c## Life Science Research/Solutions/Technologies/Protein Electrophoresis/Protein Sample Preparation ->MTS::LUSP108WI##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B##Life Science Research/Solutions/Technologies/Western Blotting ->MTS::LUSPPAKG4##Life Science Research/Solutions/Technologies/Imaging and Analysis ->MTS::LUSQC6MNI##Life Science Research/Solutions/Technologies/Surface Plasmon Resonance ->MTS::LUSM664EH## Karen Moss 12/30/11 03:16 PM 12/30/21 03:27 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Technologies/Chromatography N 0
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