Disease Diagnosis Using Chromatography

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Overview

Chromatographic analysis of plasma is an extremely useful diagnostic tool. In this section, we present an example of how chromatography was used to analyze disease profiles.

 

Related Topics:  Antibody Purification, Histidine-Tagged Recombinant Protein Purification and On-Column Refolding, Chromatography in Food Production, and Analysis of Wine Fermentation Using Chromatography.

 

Plasma Profiles Generated Using Aminex® HPX-87H Column

An increase in serum lactate and pyruvate levels is associated with several disorders. High serum lactate levels can indicate lactic acidosis in diabetes, and can be a useful prognostic indication in myocardial infarction complicated by shock. A rise in serum pyruvate levels can indicate heavy metal poisoning or thiamine deficiency. The Aminex HPX-87H column can solve many of the analytical problems associated with the determination of serum lactate and pyruvate. The figure below shows lactate, pyruvate, and urate levels in normal plasma, alongside six additional plasma samples indicative of various diseases. As the chromatograms clearly demonstrate, the Aminex HPX-87H column provides the most convenient method for analysis of acids in biological samples.

Plasma profiles generated using Aminex HPX-87H column

Plasma profiles generated using Aminex HPX-87H column. A, normal, 0.7 ml plasma; B, methylmalonic acidemia, 0.5 ml plasma; C, propionic acidemia 0.7 ml plasma; D, maple syrup urine disease, 0.4 ml plasma; E, severe ketosis, 0.1 ml plasma; F, isovaleric acidemia, 0.5 ml plasma; G, 3-hydroxy-3-methylglutaryl-CoA lyase deficiency, 0.4 ml plasma.

 

References

Bio-Rad (1999). Guide to Aminex HPLC columns for food and beverage, biotechnology, and bio-organic analysis. Bio-Rad Bulletin 1928.

Daish P and Leonard JV (1985). Rapid profiling of plasma organic acids by high performance liquid chromatography. Clinica Chimica Acta 146, 87–91.

 
MA7JNPE8Z [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [52.91.245.237, 10.232.3.134] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/applications-technologies/disease-diagnosis-using-chromatography] [x-amzn-trace-id] = [Root=1-5c15f529-a522b641d0c7ae0609c73990] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=MA7JNPE8Z] [host] = [10.232.16.28:1776] [x-request-uri] = [/en-us/applications-technologies/disease-diagnosis-using-chromatography] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/disease-diagnosis-using-chromatography LSR MA7JNPE8Z Disease Diagnosis using Chromatography Disease Diagnosis Using Chromatography /webroot/web/html/lsr/solutions/applications/protein_purification <p>Chromatographic analysis of plasma is an extremely useful diagnostic tool. In this section, we present an example of how chromatography was used to analyze disease profiles.</p> <p>&nbsp;</p> <p><strong>Related Topics:</strong>&nbsp; <a href="/evportal/destination/solutions?catID=MA7DUU15">Antibody Purification</a>, <a href="/evportal/destination/solutions?catID=MA7DZ94VY">Histidine-Tagged Recombinant Protein Purification and On-Column Refolding</a>, <a href="/evportal/destination/solutions?catID=MA7JLH15">Chromatography in Food Production</a>, and <a href="/evportal/destination/solutions?catID=MA7JPD4VY">Analysis of Wine Fermentation Using Chromatography</a>.</p> Plasma Profiles Generated Using Aminex<sup>&reg;</sup> HPX-87H Column <p>An increase in serum lactate and pyruvate levels is associated with several disorders. High serum lactate levels can indicate lactic acidosis in diabetes, and can be a useful prognostic indication in myocardial infarction complicated by shock. A rise in serum pyruvate levels can indicate heavy metal poisoning or thiamine deficiency. The <a href="/evportal/destination/commerce/sku_detail?productID=125-0140">Aminex HPX-87H column</a> can solve many of the analytical problems associated with the determination of serum lactate and pyruvate. The figure below shows lactate, pyruvate, and urate levels in normal plasma, alongside six additional plasma samples indicative of various diseases. As the chromatograms clearly demonstrate, the Aminex HPX-87H column provides the most convenient method for analysis of acids in biological samples.</p> <p><img src="/webroot/web/images/lsr/solutions/applications/protein_purification_and_isolation/application_detail/plasma-profiles-generated-using-aminex-hpx-87h-column.jpg" alt="Plasma profiles generated using Aminex HPX-87H column" width="497" height="890" /></p> <p class="caption"><strong>Plasma profiles generated using Aminex HPX-87H column.</strong> <strong>A,</strong> normal, 0.7 ml plasma; <strong>B,</strong> methylmalonic acidemia, 0.5 ml plasma;<strong> C,</strong> propionic acidemia 0.7 ml plasma; <strong>D,</strong> maple syrup urine disease, 0.4 ml plasma;<strong> E,</strong> severe ketosis, 0.1 ml plasma; <strong>F,</strong> isovaleric acidemia, 0.5 ml plasma; <strong>G,</strong> 3-hydroxy-3-methylglutaryl-CoA lyase deficiency, 0.4 ml plasma.</p> <div class="top"><a href="#helptop">Back to Top</a></div> References <p>Bio-Rad (1999). Guide to Aminex HPLC columns for food and beverage, biotechnology, and bio-organic analysis. Bio-Rad Bulletin 1928.</p> <p>Daish P and Leonard JV (1985). Rapid profiling of plasma organic acids by high performance liquid chromatography. Clinica Chimica Acta 146, 87&ndash;91.</p> <div class="top"><a href="#helptop">Back to Top</a></div> Life Science Research/Products/Chromatography/Chromatography Media/Size Exclusion Chromatography Media/Bio-Gel A Agarose Gel ->MT::7d922386-85be-4295-a797-e574d7b5f4f6##Life Science Research/Products/Chromatography/Chromatography Columns/Aminex HPLC Columns/Aminex Organic Acid and Alcohol Columns ->MT::cdc9d572-65ea-4e55-8e3a-dda17b1aa1ea##Life Science Research/Products/Chromatography/Chromatography Media/Affinity Chromatography Media/Profinity IMAC Resins ->MT::e54ab03c-d281-4e6b-aa0d-193b7737626d##Life Science Research/Products/Chromatography/Chromatography Media/Anion Exchange Chromatography Media/UNOsphere Anion Exchange Media ->MT::36286010-253e-4553-b13d-8b5917f8e93a## Karen Moss 09/19/12 05:32 PM 09/19/22 05:35 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Applications/Protein_Purification_and_Isolation N 0
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