Gravity chromatography is appropriate for laboratory-scale separations and involves minimal instrumentation. Gravity chromatography relies primarily on hand-packed columns and is particularly well suited for single-step purifications like desalting and affinity applications. This section provides an overview of gravity chromatography and selection guides to help you build your own gravity chromatography setup.
Related Topics: Low Pressure Chromatography, Medium Pressure Chromatography
As pictured to the right, a typical gravity column involves a buffer reservoir at the top of the column with flow controlled using a stopcock or a tubing pincher at the column outlet. There are a number of refinements available to assist with the general design:
Advantages of Gravity Chromatography
Gravity chromatography is:
Reproducibility is an issue if care is not taken to normalize methods. Manual sample loading can be a challenge due to the disruption of the column bed both before and after sample loading. Use a flow adaptor to minimize these effects.
Adjusting flow rate
Elution and fraction collection
Bio-Rad offers a complete line of prepacked and empty columns for gravity chromatography. Use the selection tables below to help find the best gravity chromatography setup for your application.
Empty Column Selection Guide
Prepacked and empty columns.
Glass Econo-Column Columns Selection Guide Choose the correct Econo-Column chromatography column by length and inner diameter The corresponding flow adapter is shown with the columns. A flow adapter is not needed for ID's of 0.5 or 0.7 cm. A dash ( – ) indicates that the column is not available in that configuration (L, ID, or package type).
Jacketed Econo-Column Chromatography Columns Selection Guide Choose the correct jacketed glass Econo-Column chromatography column by length and inner diameter. The corresponding flow adapter is shown with the columns. A dash ( – ) indicates that the column is not available in that configuration (L, ID, or package type).
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