Mini-PROTEAN® TGX™ and TGX Stain-Free™ Precast Mini Gels
Criterion™ TGX™ and TGX Stain-Free™ Midi Precast Gels
Superior resolution of a variety of samples. Precision Plus Protein™ unstained standards were loaded in a volume of 5 µl in each lane of a 4–20% Criterion™ TGX Stain-Free™ gel. The gel was run in a Criterion™ cell at 200 V for 45 min. A, protein bands were visualized using the Criterion Stain Free™ imager; B, the separated proteins were then transferred on to an Immun-Blot® PVDF membrane using Towbin buffer (with 20% methanol) in a Criterion blotter at 100 V for 1 hr. The transferred proteins on the membrane were also visualized using the Criterion Stain Free imager to confirm transfer efficiency; C, the gel after transfer was checked for non-transferred proteins; D, the blot was probed against 1:20,000 dilution of Streptactin-HRP conjugate, developed with Immun-Star™ Western C™ substrate, and visualized on a Molecular Imager® ChemiDoc™ XRS system.
*The Criterion Stain Free imaging system has been replaced by the Gel Doc™ EZ imaging system, which has the same performance characteristics for imaging stain-free gels but has a broader range of imaging applications.
The new Criterion™ TGX (Tris-Glycine eXtended) Stain-Free™ precast gels for PAGE are based on the long shelf life TGX formulation and include unique trihalo compounds that allow rapid fluorescent detection of proteins with the Gel Doc™ EZ and ChemiDoc™ MP imaging systems.*
TGX Stain-Free gels retain Laemmli-like separation characteristics using standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min using the Criterion electrophoresis cell and then visualized with the Gel Doc EZ and ChemiDoc MP imagers in as little as 2.5 min. The trihalo compounds react with tryptophan residues in a UV-induced reaction to produce fluorescence, which can be easily detected by the Gel Doc EZ and ChemiDoc MP imagers within gels or on a PVDF membrane after transfer.
*These gels can also be imaged on the discontinued Criterion Stain Free™ imaging system, which has been replaced by the Gel Doc EZ and ChemiDoc MP imaging systems.
What is the education discount policy?
For more than 15 years, Bio-Rad has made science education a major priority. To support
this effort, the company has implemented a discount policy that allows high school
and college teaching laboratories to purchase kits, instruments, reagents, and other
equipment at preferred prices.
How do I apply for the education discount?
If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.