C1000 Touch™ Thermal Cycler

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Overview

Specifications
 
C1000 Touch™ Thermal Cycler
Input power 850 W maximum
Frequency 50–60 Hz, single phase
Display 8.5 in. LCD display and touch screen
Ports 5 USB A, 1 USB B
Fuses Two 6.3 A, 250 V, 5 x 20 mm
Memory >1,000 typical programs onboard; unlimited with USB flash drive expansion
Dimensions (W x D x H) 33 x 46 x 20 cm (13 x 18 x 8")
Weight 10 kg (23 lb)
Temperature control modes Calculated and block
PCR license Yes
Programming options Step-based graphical and automatic
Security features Optional log-in required mode for regulated environments
Reporting Exportable run logs, system error logs
Onboard software Windows CE 6.0
PC compatibility Windows XP or higher
USB peripheral compatibility Mouse, USB flash drive, bar code reader
Real-time PCR upgrade 6-channel CFX96™ optical reaction module
5-channel CFX384™ optical reaction module
Instant incubation Yes

 

Reaction Module Specifications

 
96-Well Fast

96–Deep Well

Dual 48/48-Well Fast

384-Well
Sample capacity 96 x 0.2 ml tubes or
1 x 96-well plate
96 x 0.2 ml tubes, 48 x 0.5 ml tubes, or
1 x 96-well plate
2 x 48 x 0.2 ml tubes or
2 x 48-well plates
1 x 384-well plate
Maximum ramp rate 5°C/sec 2.5°C/sec 4°C/sec 2.5°C/sec
Average ramp rate 3.3°C/sec 2°C/sec 3°C/sec 2°C/sec
Temperature range 0–100°C 0–100°C 0–100°C 0–100°C
Temperature accuracy ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C
Temperature uniformity ±0.4°C well-to-well within 10 sec of arrival at 90°C ±0.4°C well-to-well within 10 sec of arrival at 90°C ±0.4°C well-to-well within 10 sec of arrival at 90°C ±0.4°C well-to-well within 10 sec of arrival at 90°C
Gradient capability Yes Yes Yes Yes
Gradient
Gradient accuracy ±0.2°C of programmed temperature at end rows
Row uniformity ±0.4°C well-to-well (within row) within 10 sec of arrival at target temperature
Gradient range 30–100°C
Temperature differential range 1–24°C
 

1000-series Touch Family System Tour
Fast Thermal Cycling

If you're like most researchers, you want to get results faster. Many factors contribute to the overall time it takes to get PCR results: the time to design, program, and optimize a protocol, the time to run the protocol, and the time to analyze the results. Bio-Rad engineers have developed a complete thermal cycling system that will speed every step of the PCR process to give you what's really important — a shorter time from setup to results.

Protocol autowriter — generates an optimal protocol based on your polymerase, primers, and product length.

Thermal gradient feature — identifies optimal annealing temperature in a single run.

Reduced-mass sample block — fast ramping and settling produce the shortest time to target temperature available in a thermal cycler.

Intuitive software — makes programming faster and easier for PCR and real-time PCR.


The patented* reduced-mass sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced.

Superior Uniformity
Precision of the temperature steps is critical for the rate and efficiency of PCR. To obtain reliable, consistent results, all sample wells must maintain proper temperature throughout each incubation step. The C1000 Touch™ thermal cycler uses six independently controlled thermal electric modules (TEs), the heating and cooling elements of the thermal cycler, to maintain tight temperature uniformity at all points during a run — even while ramping.

Rapid Arrival at Target Temperature
A key component of overall protocol run time is the time required to reach target temperature, which is determined by the average ramp rate and the time needed for the sample block to reach thermal uniformity. Maximum ramp rate is less important because it can fluctuate significantly during the ramp. The C1000 Touch thermal cycler's temperature control produces high average ramp rates and tight uniformity during ramping to yield fast time to target temperature and faster protocol run times. Run times can be dramatically shortened — to less than 30 min — while still producing accurate quantitative results. Now you can tailor your runs around your schedule instead of tailoring your schedule around your runs.



Superior uniformity with rapid arrival at target temperature. 1000-series thermal cyclers exhibit high average ramp rates, rapid settling time, and tight thermal uniformity throughout the ramp. This graph shows the temperature measured by probes in 15 wells across a sample block. The traces are nearly indistinguishable due to the tight uniformity. Note the consistent high average ramp rate throughout heating and cooling.

* U.S. patent 7,632,464.

Thermal Gradient

Thermal Gradient for Easy Protocol Optimization

The programmable temperature gradient allows you to identify the optimal annealing temperature in a single PCR experiment by letting you test eight incubation temperatures at once.

The 1000-series gradient feature uses dynamic ramping, which means the temperature gradient forms during ramping and all wells come to the designated temperature at the same time. As a result, the incubation period is the same for all samples, which is crucial for transfer of gradient to nongradient protocols.

The O-ring hermetic seal extends the life of the heating and cooling elements. Condensation from constant heating and cooling can degrade thermal electric modules (TEs), causing them to fail. Bio-Rad’s 1000-series cyclers include a patented* O-ring seal (red in illustration) that forms an airtight barrier around the TEs, preventing exposure to condensation. This substantially lengthens the life of TEs and guarantees outstanding thermal performance.

* U.S. patent 7,051,536.

Flexibility

The Ultimate in Configuration Flexibility

Bio-Rad's 1000-series thermal cycling platform offers maximum flexibility, so you can build a thermal cycling system that meets all your needs.

  • 6 interchangeable reaction modules, including 96-well and 384-well optical reaction modules for real-time PCR
  • USB connections for plug-and-play networking
  • Optional CFX Manager™ software for multi-instrument control by a PC
  • USB flash drive for transfer of protocols and other data between a remote PC running CFX Manager software and a C1000 Touch™ thermal cycler with a CFX96™ or CFX384™ optical reaction module
  • Multi-instrument control of up to 32 thermal cyclers
  • Multi-instrument control of up to 4 real-time PCR detection systems

USB flash drive

CFX96 optical reaction module


CFX384 optical reaction module


96-well fast reaction module, gradient enabled


96–deep well reaction module, gradient enabled

Dual 48/48-well fast reaction module, gradient enabled

384-well reaction module,
gradient enabled

Configuration Options

Stand-Alone

  • Stand-alone thermal cyclers and real-time PCR detection systems

 


Stand-Alone Multi-Instrument

  • C1000 Touch cycler control of up to 3 S1000™ cyclers
  • CFX96 Touch™ or CFX384 Touch™ system control of up to 3 S1000 cyclers

 


Software-Controlled Stand-Alone

  • C1000 Touch cycler or CFX96 Touch or CFX384 Touch system controlled by CFX Manager software

 


Software-Controlled Multi-Instrument

  • Up to 8 C1000 Touch cyclers, each connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • 1 CFX96 Touch or CFX384 Touch system connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • Up to 4 CFX96 Touch and/or CFX384 Touch systems controlled by CFX Manager software

 

Protocol Autowriter
With the Protocol Autowriter, Protocol Setup Is As Easy As 1, 2, 3

Using the right cycling protocol can have a great impact on the efficiency of your PCR. The C1000 Touch™ thermal cycler's protocol autowriter sets a new standard for easy programming by generating a customized temperature protocol based on your enzyme type, PCR product length, and primer annealing temperature. Using established PCR guidelines, the protocol autowriter suggests a cycling protocol with hot start, initial denaturation, annealing/extension steps, and final extension. It can even create a fast protocol for you, so you can get results faster.

1. Enter the polymerase, amplicon length, and primer annealing temperature (Ta).

2. Select the protocol speed — standard, fast, or ultrafast.

3. Run, edit, or save the suggested protocol.



Protocols generated by the protocol autowriter at various standard (S), fast (F), and ultrafast (U) settings yield comparable results. 2 kb, 10 kb, and 20 kb fragments were amplified from a DNA template by iProof DNA polymerase, and various fragments up to 501 bp in length were amplified from human genomic DNA using iTaq DNA polymerase. Equal amounts of reaction mixture were loaded into the well for each set of amplicons. M, markers.

Instrument Compatibility Chart

Instrument Compatibility of PCR Plastic Consumables

 

Thermal Cyclers
 
MJ Mini™ Gradient Thermal Cycler

T100™ Thermal Cycler

DNA Engine® Thermal Cycler Family

C1000 Touch™/S1000™ Thermal Cyclers
Tubes
0.5 ml individual tubes   Deep well
module only
0.2 ml individual tubes
High-profile tube strips
Low-profile tube strips  
48- and 96-Well Plates
Hard-Shell® low-profile 96-well semi-skirted PCR plates      
Hard-Shell high-profile 96-well semi-skirted PCR plates  
Hard-Shell low-profile 96-well skirted PCR plates    
Multiplate™ high-profile unskirted PCR plates
Multiplate low-profile unskirted PCR plates  
iQ™ 96-well semi-skirted PCR plates  
384-Well Plates
Hard-Shell 384-well skirted PCR plates    
Lids
Domed cap strips
Optical flat cap strips
Adhesive Seals
Microseal® 'A' film  
Microseal 'B' adhesive seals  
Microseal ꞌCꞌ optical seals  
Microseal 'F' foil  
Heat Sealer and Heat Sealing Films and Foils
PX1™ PCR plate sealer  
Optically clear heat seal  
Pierceable foil heat seal  
Peelable foil heat seal  

The C1000 Touch thermal cycler offers superior performance and a large color touch screen for easy programming. This fully modular platform is able to accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds without tools. Each PCR module has a fully adjustable heated lid that supports a wide range of vessels and sealers, including low-profile and standard-height PCR plates.

Features and Benefits

  • Quick and easy protocol programming — 2 programming options, including the Protocol Autowriter, which automatically generates customized standard, fast, and ultrafast protocols
  • Easy protocol editing — the large color touch screen and redesigned navigation make editing and running protocols easy
  • Flexible platform — change throughput capability by linking up to 3 additional S1000™ thermal cyclers or adding PC control for up to 32 cyclers
  • Advanced file management — USB flash drive compatibility allows universal protocol transfer and unlimited data storage
  • Thermal gradient — quickly and easily identify optimal annealing temperatures by using the programmable temperature gradient

Applications and Uses

  • Amplification/PCR
  • Cloning
  • Cycle sequencing
  • Gene expression studies
  • Mutagenesis

The C1000 Touch thermal cycler can be used with six interchangeable reaction modules, including:

  • A gradient-enabled 96-well fast module
  • A gradient-enabled 96–deep well module
  • A gradient-enabled dual 48/48-well fast module that allows 2 independently controlled protocols to be run side by side in a single bay
  • A gradient-enabled 384-well module for high throughput
  • 2 modules for real-time PCR detection

More Information

Select from our complete line of thermal cyclers using the PCR Thermal Cycler Selection Guide.

Thermal Cycler C1000 Touch™
Input power, W, maximum 850
Frequency, Hz, single phase 50–60
High resolution color display, cm 12 x 9
Ports (USB A / USB B) 5/1
Fuses — 2 (A/V/mm) 10/250/20
Onboard memory, protocols >1,000
Dimensions (W x D x H), cm/in 33 x 46 x 20/13 x 18 x 8
Weight, kg/lb 10/23
Temperature control modes Calculated and block
PCR license Yes
Security features (password protection, optional log-in, and secure mode) Yes
Reporting (exportable run logs and system error logs) Yes
Onboard software, Windows CE 6.0 Yes
PC compatibility (Windows XP or higher) Yes
USB peripheral compatibility (mouse, USB flash drive, bar code reader) Yes
Real-time PCR upgrade Yes
Instant incubate Yes
C1000 Touch™ Thermal Cycler with Dual 48/48 Fast Reaction Module

185-1148
Modular thermal cycler platform, includes C1000 Touch thermal cycler chassis, dual 48/48 fast reaction module, USB flash drive

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C1000 Touch™ Thermal Cycler with 96-Well Fast Reaction Module

185-1196
Modular thermal cycler platform, includes C1000 Touch thermal cycler chassis, 96-well fast reaction module, USB flash drive

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C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module

185-1197
Modular thermal cycler platform, includes C1000 Touch thermal cycler chassis, 96–deep well reaction module, USB flash drive

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C1000 Touch™ Thermal Cycler with 384-Well Reaction Module

185-1138
Modular thermal cycler platform, includes C1000 Touch thermal cycler chassis, 384-well reaction module, USB flash drive

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Dual 48/48 Fast Reaction Module

184-0148
Independent dual 48-well reaction module, fits C1000™, C1000 Touch™, and S1000™ thermal cyclers, gradient enabled

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96-Well Fast Reaction Module

184-0196
96-well fast reaction module, fits C1000™, C1000 Touch™, and S1000™ thermal cyclers, gradient enabled

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96–Deep Well Reaction Module

184-0197
96–deep well reaction module, fits C1000™, C1000 Touch™, and S1000™ thermal cyclers, gradient enabled

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384-Well Reaction Module

184-0138
384-well reaction module for high throughput, fits C1000™, C1000 Touch™, and S1000™ thermal cyclers, gradient enabled

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C1000™ Thermal Cycler Chassis

184-1000
Modular thermal cycler chassis, full-color interface, includes USB flash drive, power cord; does not include reaction module

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C1000 Touch™ Thermal Cycler Chassis

184-1100
Modular thermal cycler chassis, full-color touch screen interface, includes USB flash drive, power cord; does not include reaction module

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Touch-Screen Protector

184-9010
Pkg of 2, optional clear plastic cover for the C1000 Touch™ thermal cycler that shields the touch-screen surface from dust, reagents, and scratches

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Tube Frame

184-9000
Tube frame to provide extra support for individual 0.2 ml tubes in the dual 48- and 96-well fast reaction modules

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Tube Frame

184-9001
Tube frame to provide extra support for individual 0.2 ml tubes in the 96–deep well reaction module

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USB Cable

184-8000
USB cable for use with C1000™, C1000 Touch™, and S1000™ thermal cyclers

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1000-Series Connectivity Kit

184-1001
Kit contains mouse, mouse pad, USB flash drive

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Number Description Options
10021377 Instruction Manual, C1000 Touch Thermal Cycler, Rev B Click to download
10022527 Amplification Plastics and Reagents Selection Guide, Rev A Click to download
5277 Bio-Rad Thermal Cyclers Brochure, Rev F Click to download [ Add to Cart (Free) ]
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]
10021427 Instruction Manual, C1000 Touch Thermal Cycler Quick Guide, Rev A Click to download
6085 C1000 Touch Thermal Cycler Flier, Rev A Click to download [ Add to Cart (Free) ]
6094 1000-Series Thermal Cyclers Brochure, Rev B Click to download [ Add to Cart (Free) ]
6095 C1000 Touch Thermal Cycler Specifications Sheet, Rev B Click to download [ Add to Cart (Free) ]
10022138 Instruction Manual, Checking Firmware and Software Updates for the C1000 Touch Thermal Cycler and the CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems — A Quick Guide, Rev B Click to download