S3™ Cell Sorter

S3 Cell Sorter


Data Analysis on the S3™ Cell Sorter



HeLa cells were transfected with GFP and sorted by forward and side scatter, singlets, and GFP expression. A–B, presort analysis of GFP-positive cells; C–D, postsort population was sorted to >98% purity. Postsort analysis revealed that a pure GFP-positive population was successfully sorted.

Sensitivity of fluorescent microspheres

Sensitivity of fluorescent microspheres

SPHERO rainbow calibration particles (8 peaks) were excited by both 488 nm and 561 nm lasers. Emission was measured by all four detectors. Between peaks, all channels displayed a coefficient of variation (CV) <2.5 units. The FL3 channel displays the seven lowest peaks. The eighth peak is offscale due to the brightness of the signal intensity.

Cleaning system

Cleaning System

Carryover between samples is minimized using the “lock and load” backflushing system. FITC-labeled beads were run on the instrument (A). Subsequently, PE-labeled beads were run (B), followed by a repeated cycle of FITC-labeled beads (C) and PE-labeled beads (D). Each time, carryover of fluorescently labeled beads was analyzed and showed minimal carryover between samples.

Automated Drop Delay Measurement and Monitoring on the S3™ Cell Sorter

Optimal Cell Sorting Performance
and Purity

Obtaining optimal sort purity and maximizing recovery requires accurate drop delay determination, or measurement of the distance between the laser point of analysis and the last connected drop.

Historically, the drop delay process has been labor intensive and has required bead counting on a microscope or image processing with a camera.

ProDrop™ Technology automates drop delay determination and accurately calculates drop delay values, resulting in less opportunity for error and leading to better recovery. The exact drop delay value is calculated by measuring the number of ProLine™ Calibration Beads in the waste stream during the QC process.

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S3™ Biosafety System Class I

Easy access to the S3 and S3e™ Cell Sorters:


Easy access to the S3 cell sorter

The S3 cell sorter is an automated benchtop cell sorter featuring either one or two lasers and up to four fluorescence detectors plus forward- and side-scatter detectors. The S3 cell sorter uses state-of-the-art ProDrop™ technology for automated drop delay calculation and droplet break-off monitoring, thereby simplifying one of the most complex and error-prone aspects of cell sorting.

This affordable cell sorter provides exceptional sort purity without compromising performance and sensitivity. The system is ideal for sorting cells expressing fluorescent proteins such as eGFP, DsRed, mCherry, or for cells labeled with fluorescent markers such as labeled antibodies.

Features and Benefits

  • Simplified instrument setup — automated drop delay calculation and droplet break-off monitoring enables precise 1–4 color sorting with minimal training
  • Compact design — dimensions of only 70 × 65 × 65 cm (2.3 × 2.1 × 2.1 ft) including onboard fluidics and temperature control system
  • Reduced carryover — dual-position loading stage ensures the sample line remains clean between sorting runs
  • 2-way cell sorting — sort two different defined populations at the same time
  • High speed and high purity — sort cells fast while maintaining high sensitivity and purity
  • Intuitive interface — user-friendly ProSort™ software enables effortless instrument control and sort logic definition

For added biosafety during the cell sorting process, the optional S3 biosafety system Class I (145-1078) is available. Custom-designed to work specifically with the S3 cell sorter, the biosafety system Class I provides protection for both the user and the environment from aerosols created while sorting. The biosafety system is an affordable alternative to the large and expensive traditional biosafety hoods offered for cell sorters.

Acquisition rate, events/sec Up to 100,000
Sorting rate, events/sec Up to 30,000
Sorting purity >99% pure
Nozzle size, µm 100
Sorting direction 2-way sorting
Sorting collection Up to 5 x 5 ml sample tubes each direction
Up to 5 x 1.5 ml tubes each direction
Microscope slides
8-well strip each direction
Lasers Primary: 488 nm 100 mW
Optional: 561 nm 100 mW
Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescence detectors with PMT
Minimum resolution: 0.5 µm
Sensitivity, MESF <125 for FITC and PE
Temperature range, °C Sample and collection temperature control system: 4–37
Peltier solid state system
Fluidics Onboard fluidics and dilution of 8x sheath fluid with deionized water
Data format FCS 3.1
Dimensions (W x D x H), ft/cm 2.3 x 2.1 x 2.1 / 70 x 65 x 65
Number Description Options
6329 S3 Cell Sorter Flier, Rev B Click to download
6325 S3 Cell Sorter Brochure, Rev D Click to download
10032007 S3™ and S3e™ Cell Sorters Quick Guide, Rev D Click to download
10031105 Instruction Manual, S3™ and S3e™ Cell Sorters, Rev D Click to download
6404 S3™ Biosafety System Class I Flier, Rev A Click to download
6503 S3 Biosafety System Class I Brochure, Rev A Click to download
10033232 Instruction Manual, S3™ Biosafety System Class I, Rev B Click to download
10030825 ProFlow™ Sort Grade 8x Sheath Fluid Product Insert, Rev B Click to download
10041517 ProLine Universal Calibration Beads Product Insert, Rev A Click to download
10041735 S3™ Filter Block (A or B) with Dichroic Filter Holders Product Insert, Rev A Click to download
10041736 S3™ Individual Filter Holder Product Insert, Rev A Click to download
10041737 S3™ Individual Dichroic Filter Holder Product Insert, Rev A Click to download