S1000™ Thermal Cycler

S1000 Thermal Cycler – Thermal Cycler Machines


S1000™ Thermal Cycler
Input power 700 W maximum
Frequency 50–60 Hz, single phase
Display LCD
Ports 4 USB A, 1 USB B
Fuses Two 6.3 A, 250 V, 5 x 20 mm
Memory >1,000 typical programs onboard
Dimensions (W x D x H) 33 x 46 x 20 cm (13 x 18 x 8")
Weight 10 kg (23 lb)
Temperature control modes Calculated and block
PCR license Yes
Programming options Text based
Reporting No
Onboard software No
PC compatibility Windows XP or higher with C1000™ or C1000 Touch™ thermal cycler
USB peripheral compatibility No
Real-time PCR upgrade No
Instant incubation Yes


Reaction Module Specifications

96-Well Fast

96–Deep Well

Dual 48/48-Well Fast

Sample capacity 96 x 0.2 ml tubes or 1 x 96-well plate 96 x 0.2 ml tubes, 48 x 0.5 ml tubes, or 1 x 96-well plate 2 x 48 x 0.2 ml tubes or 2 x 48-well plates 1 x 384-well plate
Maximum ramp rate 5°C/sec 2.5°C/sec 4°C/sec 2.5°C/sec
Average ramp rate 3.3°C/sec 2°C/sec 3°C/sec 2°C/sec
Temperature range 0–100°C 0–100°C 0–100°C 0–100°C
Temperature accuracy ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C ±0.2°C of programmed target at 90°C
Temperature uniformity ±0.4°C well-to-well within 10 sec of arrival at 90°C ±0.4°C well-to-well within 10 sec of arrival at 90°C ±0.4°C well-to-well within 10 sec of arrival at 90°C ±0.4°C well-to-well within 10 sec of arrival at 90°C
Gradient capability Yes Yes Yes Yes
Gradient accuracy ±0.2°C of programmed temperature at end rows
Row uniformity ±0.4°C well-to-well (within row) within 10 sec of arrival at target temperature
Gradient range 30–100°C
Temperature differential range 1–24°C

1000-series Touch Family System Tour
Fast Performance

If you're like most researchers, you want to get results faster. Many factors contribute to the overall time it takes to get PCR results: the time to design, program, and optimize a protocol, the time to run the protocol, and the time to analyze the results. Bio-Rad engineers have developed a complete thermal cycling system that will speed every step of the PCR process to give you what's really important — a shorter time from setup to results.

Thermal gradient feature — identifies optimal annealing temperature in a single run.

Reduced-mass sample block — fast ramping and settling produce the shortest time to target temperature available in a thermal cycler.

The patented* reduced-mass sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced.

Superior Uniformity
Precision of the temperature steps is critical for the rate and efficiency of PCR. To obtain reliable, consistent results, all sample wells must maintain proper temperature throughout each incubation step. The S1000 thermal cycler uses six independently controlled thermal electric modules (TEs), the heating and cooling elements of the thermal cycler, to maintain tight temperature uniformity at all points during a run — even while ramping.

Rapid Arrival at Target Temperature
A key component of overall protocol run time is the time required to reach target temperature, which is determined by the average ramp rate and the time needed for the sample block to reach thermal uniformity. Maximum ramp rate is less important because it can fluctuate significantly during the ramp. The S1000 thermal cycler's temperature control produces high average ramp rates and tight uniformity during ramping to yield fast time to target temperature and faster protocol run times. Run times can be dramatically shortened — to less than 30 min — while still producing accurate quantitative results. Now you can tailor your runs around your schedule instead of tailoring your schedule around your runs.

Superior uniformity with rapid arrival at target temperature. 1000-series thermal cyclers exhibit high average ramp rates, rapid settling time, and tight thermal uniformity throughout the ramp. This graph shows the temperature measured by probes in 15 wells across a sample block. The traces are nearly indistinguishable due to the tight uniformity. Note the consistent high average ramp rate throughout heating and cooling.

* U.S. patent 7,632,464.

Thermal Gradient

Thermal Gradient for Easy Protocol Optimization

The programmable temperature gradient allows you to identify the optimal annealing temperature in a single PCR experiment by letting you test eight incubation temperatures at once.

The 1000-series gradient feature uses dynamic ramping, which means the temperature gradient forms during ramping and all wells come to the designated temperature at the same time. As a result, the incubation period is the same for all samples, which is crucial for transfer of gradient to nongradient protocols.

The O-ring hermetic seal extends the life of the heating and cooling elements. Condensation from constant heating and cooling can degrade thermal electric modules (TEs), causing them to fail. Bio-Rad’s 1000-series cyclers include a patented* O-ring seal (red in illustration) that forms an airtight barrier around the TEs, preventing exposure to condensation. This substantially lengthens the life of TEs and guarantees outstanding thermal performance.

* U.S. patent 7,051,536.


The Ultimate in Configuration Flexibility

S1000™ thermal cyclers are compatible with 96-well fast, 96–deep well, dual 48/48-well fast, and 384-well reaction modules.

96-well fast reaction module, gradient enabled

96–deep well reaction module, gradient enabled

Dual 48/48-well fast reaction module, gradient enabled

384-well reaction module, gradient enabled

Configuration Options


  • Stand-alone thermal cyclers and real-time PCR detections systems


Stand-Alone Multi-Instrument

  • C1000 Touch™ cycler control of up to 3 S1000 cyclers
  • CFX96 Touch™ or CFX384 Touch™ system control of up to 3 S1000 cyclers


Software-Controlled Stand-Alone

  • C1000 Touch cycler or CFX96 Touch or CFX384 Touch system controlled by CFX Manager™ software


Software-Controlled Multi-Instrument

  • Up to 8 C1000 Touch cyclers, each connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • 1 CFX96 Touch or CFX384 Touch system connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • Up to 4 CFX96 Touch and/or CFX384 Touch systems controlled by CFX Manager software


Instrument Compatibility Chart

Instrument Compatibility of PCR Plastic Consumables


Thermal Cyclers
MJ Mini™ Gradient Thermal Cycler

T100™ Thermal Cycler

DNA Engine® Thermal Cycler Family

C1000 Touch™/S1000™ Thermal Cyclers
0.5 ml individual tubes   Deep well
module only
0.2 ml individual tubes
High-profile tube strips
Low-profile tube strips  
48- and 96-Well Plates
Hard-Shell® low-profile 96-well semi-skirted PCR plates      
Hard-Shell high-profile 96-well semi-skirted PCR plates  
Hard-Shell low-profile 96-well skirted PCR plates    
Multiplate™ high-profile unskirted PCR plates
Multiplate low-profile unskirted PCR plates  
iQ™ 96-well semi-skirted PCR plates  
384-Well Plates
Hard-Shell 384-well skirted PCR plates    
Domed cap strips
Optical flat cap strips
Adhesive Seals
Microseal® 'A' film  
Microseal 'B' adhesive seals  
Microseal ꞌCꞌ optical seals  
Microseal 'F' foil  
Heat Sealer and Heat Sealing Films and Foils
PX1™ PCR plate sealer  
Optically clear heat seal  
Pierceable foil heat seal  
Peelable foil heat seal  

The S1000 thermal cycler combines powerful performance with streamlined features. The cycler can be used as a stand-alone instrument for PCR, or it can be connected to a C1000 Touch™ thermal cycler for additional throughput. Up to three S1000 cyclers can be connected to a C1000 Touch cycler, and the four cyclers can then be conveniently controlled as one high-throughput instrument.

Features and Benefits

  • Flexible platform — choice of 4 interchangeable reaction modules; change throughput capability by linking up to 3 S1000 cyclers to a C1000 Touch cycler or adding PC control for up to 32 cyclers
  • Exceptional thermal performance — the patented* reduced-mass honeycomb sample block delivers fast ramping and settling times for reliable results and reduced run times
  • Optimal sealing — the fully adjustable heated lid accommodates a wide range of reaction vessels
  • Thermal gradient — quickly and easily identify optimal annealing temperatures by using the programmable temperature gradient

Applications and Uses

  • Amplification/PCR
  • Cloning
  • Cycle sequencing
  • Gene expression studies
  • Mutagenesis

The S1000 cycler can be used with four interchangeable reaction modules, including:

  • A gradient-enabled 96-well fast module
  • A gradient-enabled 96–deep well module
  • A gradient-enabled dual 48/48-well fast module that allows 2 independently controlled protocols to be run side by side in a single bay
  • A gradient-enabled 384-well module

More Information

Select from our complete line of thermal cyclers using the Thermal Cycler Selection Chart.

* U.S. patent 7,632,464.

Thermal cycler S1000
Input power, W, maximum 700
Frequency, Hz, single phase 50–60
Display (LCD) Yes
Ports (USB A / USB B) 4/1
Fuses — 2 (A/V/mm) 10/250/20
Onboard memory, protocols >1,000 
Dimensions (W x D x H), cm/in 33 x 46 x 20/13 x 18 x 8
Weight, kg/lb 10/23
Temperature control modes Calculated and block
PCR license Yes
Reporting (exportable run logs and system error logs) No
Onboard software, Windows CE 6.0 No
PC compatibility (Windows XP or higher) Yes
USB peripheral compatibility (mouse, USB flash drive, bar code reader) No
Real-time PCR upgrade No
Instant incubate Yes
Number Description Options
6094 1000-Series Thermal Cyclers Brochure, Rev B Click to download [ Add to Cart (Free) ]
10022527 Amplification Plastics and Reagents Selection Guide, Rev B Click to download
5277 Bio-Rad Thermal Cyclers Brochure, Rev G Click to download [ Add to Cart (Free) ]
10010306 Instruction Manual, S1000 Thermal Cycler, Rev E Click to download [ Add to Cart (Free) ]
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]
10021463 Instruction Manual, S1000 Thermal Cycler Quick Guide, Rev A Click to download
6092 S1000 Thermal Cycler Specifications Sheet, Rev B Click to download [ Add to Cart (Free) ]
6082 S1000 Thermal Cycler Flier, Rev A Click to download [ Add to Cart (Free) ]