RC DC™ Protein Assay



Protein Concentration Test Tube Micro Test Tube
High-concentration assay 0.2–1.5 mg/ml protein 0.2–1.5 mg/ml protein
  100 µl sample 25 µl sample
  500 µl reagent I 125 µl reagent I
  500 µl reagent II 125 µl reagent II
  500 µl reagent A 125 µl reagent A
  4.0 ml reagent B 1.0 ml reagent B
Compatible Reagents – RC DC™ Protein Assay
The RC DC protein assay is compatible with the following reagents and buffers in addition to those compatible with the DC protein assay:
CHAPS 2% ReadyPrep sequential extraction reagents 2 and 3
DTT, 350 mM Sodium hydroxide, 2.5 M
EDTA, 0.1 M TBP, 2 mM
Imidazole, 0.5 M Tris, pH 8.4, 0.5 M
Laemmli buffer with 5% β-mercaptoethanol Triton X-100, 2%
β-Mercaptoethanol, 10% Tween 20, 2%

* Using standard procedure.

Protein Assay Selection Guide

Chart RC DC Protein Assay DC Protein Assay Quick Start Bradford Assay Assay Bio-Rad Protein Assay

  * Nonidet P-40 >0.25%, Triton C-100 >0.05%, Tween 20 >0.01%, SDS >0.025%
** DTT >1 mM, EDTA >1 mM

Protein Assay Comparison
Characteristics of Bio-Rad's Protein Assays
  Protein Assay
Quick Start™ Bradford Bio-Rad DC RC DC™
Adapted from method of Bradford (1976) Bradford (1976) Lowry et al. (1951) Lowry et al. (1951)
Standard concentration assay        
Sample volume 100 µl 100 µl 100 µl 100 µl
Linear range 0.125–1.5 mg/ml 0.2–1.5 mg/ml 0.125–1.5 mg/ml 0.125–1.5 mg/ml
Low-concentration assay        
Sample volume 1 ml 800 µl 200 µl 200 µl
Linear range 1.25–25 µg/ml 1.25–25 µg/ml 5–250 µg/ml 5–250 µg/ml
Microplate assay sample volume 5 µl 10 µl 5 µl *
Minimum incubation time 5 min 5 min 15 min 15 min
Assay wavelength 595 nm 595 nm 650–750 nm 650–750 nm

All assays are easy to use, require little reagent preparation, and give accurate and reproducible results.

* To adapt the RC DC assay to a microplate format, follow the micro test tube (microfuge tube) assay protocol in the RC DC instruction manual up to the centrifugation step where the supernatant is discarded. The pellet can then be transferred to the microplate, and the microplate assay protocol in the DC protein assay manual can be followed.

In a typical protein assay, a chemical reagent is added to a protein sample, producing a visible result, such as a color change in the sample solution. This color change is quantitated with a spectrophotometer or microplate reader, and compared to a standard curve of known concentrations of protein versus their absorbance after reaction with the reagent. The amount of protein in the unknown sample is determined by interpolation, reading the concentration of protein on the standard curve that corresponds to its absorbance.

The RC DC™ (reducing agent and detergent compatible) protein assay is a colorimetric assay for protein determination in the presence of reducing agents and detergents. The RC DC protein assay has all the unique features of the original DC protein assay, but covers a broader range of reagents. This assay is based on the Lowry protocol, and its expanded capabilities offer:

  • Assay compatibility with mixtures of reducing agents and detergents
  • Simple, proven protocol for protein quantitation directly in complex reagent mixtures, such as Laemmli buffer and Bio-Rad's ReadyPrep reagents

Refer to the list of compatible reagents for more information.

RC DC protein assay kits offer either bovine serum albumin or bovine γ-globulin standards.


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<em>RC DC</em>™ Protein Assay Kit I
RC DC™ Protein Assay Kit I

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, bovine γ-globulin standard; 450 standard assays

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<em>RC DC</em>™ Protein Assay Kit II
RC DC™ Protein Assay Kit II

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, BSA standard; 450 standard assays

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Number Description Options
1123 Protein Assay Applications Bibliography Click to download
1069 Colorimetric Protein Assays, Rev D Click to download
2610 RC DC Protein Assay Product Information Sheet, Rev D Click to download
4110107 Instruction Manual, RC DC Protein Assay, Rev A Click to download
6838 RC DC™ Protein Assay Quick Guide, Ver A Click to download