PV92 PCR Informatics Kit

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PV92 PCR Informatics Kit

Overview

Purchasing Guide Table

Reduce. Reuse. Refresh.

Refresh kit components, reduce packaging waste, reuse components, and refresh your kits, and you’ll save storage space by purchasing individual items. Bio-Rad now has many individual components for Biotechnology Explorer™ kits available for purchase.


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Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Positive controls: homozygous (+/+), homozygous (–/–), heterozygous (+/–) 1 each
PCR master mix (Taq DNA polymerase, dNTPs, buffer) 1
PCR primers 1
DNA molecular mass ruler 1
InstaGene™ DNA extraction matrix, 20 ml 1
Xylene cyanole DNA loading dye, 5x, 1 ml 1
Microcentrifuge tubes, 1.5 ml 60
PCR tubes, 0.2 ml 50
Screwcap microcentrifuge tubes, 1.5 ml 50
Capless PCR tube adaptors, 1.5 ml 50
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Foam floats 8
Gel staining trays 4
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Protease solution (for extraction from hair follicles)
1
Horizontal gel electrophoresis chambers
4–8
Adjustable micropipets  
   2–20 µl
1–8
   20–200 µl and 100–1,000 µl 1 each
Pipet tips, aerosol barrier  
   2–1,000 µl, Xcluda B, D and E  
Microcentrifuges 1–4
Thermal cycler 1
Power supplies 2–4
Recommended (Optional) Accessories:
Water bath or dry bath
Rocking platform
Gel support film
Vortexer
Microwave oven
PV92 PCR Informatics Kit Flowchart
PV92 PCR Informatics Flowchart
Key Kit Features
  • Grow a bacterial library
  • Clone a gene of interest in bacteria
  • Express a protein of interest in bacteria
  • Purify a protein of interest from bacteria
  • Take a protein to market
  • Complete in four 45 min lab sessions
Curriculum Fit
Conventional PCR Animation

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Compare DNA Samples via Electrophoresis
PV92 PCR Informatics Gel

Visualize and compare DNA samples as part of the PV92 PCR Informatics kit workflow.

Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.


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Complete Purchasing Guide

Download the complete Biotechnology Explorer™ Refresh Kit Components Purchasing Guide.


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PowerPoint Presentation for Workshops and for Teaching

PV92 PCR Informatics Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the PV92 PCR Informatics Kit and includes instructions for using the kit.

PV92 PCR Informatics Kit Download PowerPoint presentation
PV92 PCR Informatics Kit

With the PV92 PCR Informatics Kit, your students use real–world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus. Using their own results, students test the Hardy–Weinberg equilibrium theory within their classroom population, then go online to compare their classroom results to genetic data of populations worldwide.

Features and Benefits

  • Aligns with AP Biology Lab 8
  • Introduces PCR and applies it to population genetics

With this kit, students are able to:

  • Directly measure human diversity at the molecular level
  • Extract and amplify genomic DNA from their own samples
  • Compare results to online data

Background Information

In this activity students hunt for a specific Alu repeat (a 300 base pair repetitive sequence of DNA) within a specific region called PV92 on chromosome 16. Over evolutionary time, up to 1 million copies of the Alu repeat have become randomly inserted throughout the human genome. Some of us carry an Alu insertion and some of us do not. Such variations among individuals' genotypes are inherited — and are the raw material of genetic diversity and evolution. These subtle variations in our DNA are evidence of our ancestry and form the basis of personal identification via DNA fingerprinting.

More Information

  • Three–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Positive controls: homozygous (+/+), homozygous (-/-), heterozygous (+/-) 1
PCR master mix (Taq DNA polymerase dNTPs, buffer) 1
PCR primers 1
DNA molecular mass ruler 1
InstaGene™ DNA Extraction Matrix, 20 ml 1
Xylene cyanole, DNA loading dye, 5x, 1 ml 1
Micro test tubes, 1.5 ml 60
PCR tubes, 0.2 ml 50
Screwcap tubes, 1.5 ml 50
Capless PCR tube adaptors, 1.5 ml 50
Agarose powder, 5 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA Stain, 500x, 100 ml 1
Foam micro test tube holders 8
Gel-staining trays 4
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
PV92 PCR Informatics Kit

166-2100EDU
PCR classroom study kit, includes positive controls, PCR master mix, primers, extraction matrix, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students; education use only

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Number Description Options
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download
4110053 Getting the Most out of the PV92 PCR Informatics Kit, Biotechnology Explorer, Rev C Click to download [ Add to Cart (Free) ]
4110052 Instruction Manual, Chromosome 16: PV92 PCR Informatics Kit, Biotechnology Explorer, Rev F Please login to download this document [ Add to Cart (Free) ]
3058 Instruction Manual, PV92 PCR Informatics Kit Quick Guide, Biotechnology Explorer, Rev B Click to download
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide, Rev A Click to download
5872 Biotechnology Explorer Refresh Kit Components Purchasing Guide, Rev A Click to download