ProteOn™ Sensor and Maintenance Chips

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Overview

ProteOn™ LCP Sensor Chip
ProteOn LCP Sensor Chip


For capturing lipid assemblies such as liposomes.
Applications: The LCP sensor chip features a self-assembled NeutrAvidin layer in a planar configuration suitable for lipid-protein, lipid–small molecule, and membrane protein–protein interaction analysis.

ProteOn GLC Sensor Chip

For general amine coupling: compact polymer layer with binding capacity of approximately one protein monolayer. Suitable for various applications, including protein-protein interaction analysis.

ProteOn GLM Sensor Chip

For general amine coupling: extended polymer matrix with intermediate binding capacity for high response. Suitable for various applications, including protein-small molecule and protein-protein interaction analysis.

ProteOn GLH Sensor Chip

For general amine coupling: highly extended polymer layer for maximum binding capacity. Suitable for protein-small molecule and protein-protein interactions where highest sensitivity is the primary objective.

ProteOn NLC Sensor Chip

For binding of biotinylated molecules: NeutrAvidin immobilized to GLC layer. Suitable for various applications, including protein-DNA and protein-protein interaction analysis.

ProteOn™ HTG and HTE Sensor Chips

ProteOn HTG Sensor Chip

For binding histidine-tagged proteins/ligands: tris-NTA complexes are attached to a polymer matrix layer and are activated by injection of nickel (II) ions.
Applications: The HTG sensor chip features a low-density tris-NTA surface suitable for protein-protein, protein-peptide, and protein-DNA interaction analysis.

 

ProteOn HTE Sensor Chip

For binding histidine-tagged proteins/ligands: tris-NTA complexes are attached to an alginate matrix layer and are activated by injection of nickel (II) ions.
Applications: The HTE sensor chip features a high-density tris-NTA surface suitable for protein-peptide, protein-DNA, and protein–small molecule interaction analysis.

Used with the ProteOn XPR36 protein interaction array system, ProteOn sensor chips combine advanced surface chemistries with state-of-the-art instrument microfluidics. Robust, real-time, label-free biomolecular interaction data are generated using this novel 6 × 6 surface plasmon resonance (SPR) technology.

Features and Benefits of ProteOn Sensor and Maintenance Chips

  • Low cost per data point
  • Range of surfaces designed for diverse protein interaction studies
  • Easy optimization of capture surfaces using validated protocols
  • Maintenance chip and protocols keep your system in excellent working order

Applications and Uses of Surface Plasmon Resonance Technology

NEW! ProteOn LCP Sensor Chip
The LCP sensor chip utilizes a NeutrAvidin-coated surface with a planar configuration. This SPR chip is designed for use with the ProteOn LCP reagent kit to capture both single and multiple layers of lipid assemblies such as liposomes.

ProteOn GLC Sensor Chip
The GLC sensor chip is designed for general amine coupling utilizing a compact polymer layer with a binding capacity of approximately one protein monolayer. This SPR chip is most suitable for protein-protein interaction analysis.

ProteOn GLM Sensor Chip
The GLM sensor chip is designed for general amine coupling and has an extended polymer matrix with intermediate binding capacity for high response. This SPR chip is suitable for both protein–small molecule and protein-protein interaction analyses.

ProteOn GLH Sensor Chip
The GLH sensor chip is designed for general amine coupling and has a highly extended polymer layer for maximum binding capacity. This SPR chip is most suitable for protein–small molecule applications in which high sensitivity is the primary objective.

ProteOn NLC Sensor Chip
The NLC sensor chip features NeutrAvidin immobilized on a GLC polymer layer with a binding capacity of approximately one protein monolayer. This SPR chip is an ideal choice for binding biotinylated proteins, peptides, and nucleic acids.

ProteOn HTG and HTE Sensor Chips
These ProteOn sensor chips feature novel tris-NTA complexes for improved capture of polyhistidine-tagged proteins. The complexes are attached to an alginate matrix layer and are activated by injection of Ni2+ ions and regenerated by injection of EDTA.

  • ProteOn HTG sensor chip — has a lower tris-NTA surface density and less nonspecific binding, making it suitable for protein-protein and protein-peptide interaction analysis
  • ProteOn HTE sensor chip — has an elevated tris-NTA surface density optimal for protein–small molecule interaction analysis

Each ProteOn sensor chip is enclosed in a bar-coded cartridge for reliable data tracking and archiving.

ProteOn Maintenance Chips
There are two maintenance sensor chips for use in the ProteOn XPR36 system. These chips are available individually or as part of the ProteOn maintenance kit:

The ProteOn XPR36 protein interaction analysis system includes all of the components required for interaction analysis: instrumentation, ProteOn Manager™ software, ProteOn sensor chips, buffer and reagent kits, and protocol development kits.

ProteOn LCP Sensor Chip
ProteOn™ LCP Sensor Chip

176-5041
Pkg of 1, ProteOn XPR36 system sensor chip for capturing lipid assemblies such as liposomes; for use with the ProteOn LCP capturing reagent kit

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ProteOn™ HTE Sensor Chip

176-5033
Pkg of 1, higher binding capacity, ProteOn XPR36 system sensor chip for capturing histidine-tagged proteins; contains novel tris-NTA complexes

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ProteOn HTG Sensor Chip #176-5031
ProteOn™ HTG Sensor Chip

176-5031
Pkg of 1, compact binding capacity, ProteOn XPR36 system chip for capturing histidine-tagged proteins; contains novel tris-NTA complexes

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ProteOn GLC Sensor Chip #176-5011
ProteOn™ GLC Sensor Chip

176-5011
Pkg of 1, compact binding capacity, ProteOn XPR36 system chip for general amine coupling; binding capacity of approximately one protein monolayer

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ProteOn GLM Sensor Chip #176-5012
ProteOn™ GLM Sensor Chip

176-5012
Pkg of 1, intermediate binding capacity, ProteOn XPR36 system chip for general amine coupling

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ProteOn GLH Sensor Chip #176-5013
ProteOn™ GLH Sensor Chip

176-5013
Pkg of 1, high binding capacity, ProteOn XPR36 system chip for general amine coupling

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ProteOn NLC Sensor Chip #176-5021
ProteOn™ NLC Sensor Chip

176-5021
Pkg of 1, ProteOn XPR36 system chip for binding of biotinylated molecules; contains NeutrAvidin immobilized to GLC layer

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ProteOn MNT Maintenance Chip #176-5100
ProteOn™ MNT Maintenance Chip

176-5100
Pkg of 1, ProteOn XPR36 system chip for use in instrument maintenance protocol

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ProteOn CLN Cleaning Chip #176-5110
ProteOn™ CLN Cleaning Chip

176-5110
Pkg of 1, ProteOn XPR36 system chip for use in microfluidics network cleaning protocol

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Number Description Options
6132 The ProteOn HTG Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Protein Interaction Analysis, Rev A Click to download
6295 ProteOn Sensor Chips, Tips and Techniques, Rev A Click to download
5404 ProteOn Sensor Chips Product Information Sheet, Rev E Click to download [ Add to Cart (Free) ]
5409 Protein Interaction Analysis Ordering Information Sheet, Rev E Click to download [ Add to Cart (Free) ]
10024332 ProteOn Liposome Capturing Kit Product Insert, Rev A Click to download
5948 Attachment of Membrane Protein Interactions Using Lipoparticles Bound to the Surface of the ProteOn XPR36 Sensor Chips, Rev A Click to download [ Add to Cart (Free) ]
5968 Screening and Characterization of Human Immunoglobulin G Binding Peptides Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download [ Add to Cart (Free) ]
5679 Applications of the ProteOn GLH Sensor Chip: Interactions Between Proteins and Small Molecules, Rev A Click to download [ Add to Cart (Free) ]
5540 Screening, Ranking, and Epitope Mapping of Anti-Human IL-9 Supernatants, Rev A Click to download
5449 Applications of the ProteOn NLC Sensor Chip: Antibody-Antigen, DNA-Protein, and Protein-Protein Interactions, Rev B Click to download [ Add to Cart (Free) ]
5358 Mechanisms of Protein-Protein Binding: Double-Mutant Cycle Analysis Using the ProteOn XPR36 System, Rev A Click to download
5368 Anaylsis of Multiple Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev B Click to download
5367 Rapid Optimization of Immobilization and Binding Conditions for Kinetic Analysis of Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download
5360 Rapid and Detailed Analysis of Multiple Antigen-Antibody Pairs Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download
5413 ProteOn XPR36 Hardware Product Information Sheet, Rev B Click to download
5412 Antibody Characterization and Development: ProteOn XPR36 Protein Interaction Array System Application Guide, Rev C Click to download
6161 Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download [ Add to Cart (Free) ]
6254 The ProteOn HTE Sensor Chip: Novel Surface for Stable Capture of Histidine-Tagged Proteins for Protein-Small Molecule Interaction Analysis, Rev A Click to download [ Add to Cart (Free) ]
6449 Novel Liposome-Capture Surface Chemistries to Analyze Drug-Lipid Interaction using the ProteOn XPR36 System, Rev A Click to download
6414 ProteOn™ XPR36 Experimental Design and Application Guide, Rev B Click to download