ProteOn™ Protocol Development Kits

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ProteOn protocol development kits provide the reagents and sensor chips needed to perform and analyze data from a complete experiment using the ProteOn XPR36 protein interaction array system. New users will gain familiarity with instrument setup and operation, experiment design, and data analysis. Experienced users will find the kits valuable for system benchmarking and protocol development.

ProteOn One-shot Kinetics Kit
The interaction between the cytokine IL-2 and an IL-2 antibody demonstrates a detailed kinetic analysis in a single injection cycle (One-shot Kinetics). The kit also demonstrates a useful method for controlling ligand immobilization levels during protocol development and optimization.

The ProteOn One-shot Kinetics kit includes a ProteOn IL-2/IL-2 antibody pair, with sufficient materials to immobilize IL-2 antibody in up to six channels and to analyze a complete concentration series of IL-2 across each channel.

ProteOn Multiple Protein Interaction Kit
The interaction between five mutant TEM1 β-lactamase proteins and wild-type β-lactamase inhibitor protein(BLIP) demonstrates the power of the ProteOn XPR36 system to produce a detailed kinetic analysis of multiple simultaneous interactions and to map protein interfaces.

The ProteOn multiple protein interaction kit includes a ProteOn TEM1/BLIP protein set, with sufficient materials to immobilize five TEM1 mutant proteins in five channels and to perform a detailed kinetic analysis of the interaction of each of the five mutant proteins with BLIP.

ProteOn Protein-Small Molecule Kit
The interaction between carbonic anhydrase II and 4-carboxy benzene sulfonamide (4-CBS) demonstrates the capability of the ProteOn XPR36 system to detect low molecular weight analytes. The kit also provides a benchmark for optimal system performance.

The ProteOn protein-small molecule kit includes ProteOn carbonic anhydrase II/CBS, with sufficient materials to immobilize carbonic anhydrase II in up to six channels and analyze a complete concentration series of CBS.

ProteOn™ One-shot Kinetics Kit

176-1010
ProteOn protocol development kit, includes IL-2/IL-2 antibody pair, GLC sensor chip, amine coupling kit, 50 ml Na acetate buffer, pH 4.5

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ProteOn Multiple Protein Interaction Kit

176-1020
ProteOn protocol development kit, includes TEM/BLIP protein set, GLC sensor chip, amine coupling kit, 50 ml Na acetate buffer, pH 4.0

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ProteOn™ Protein–Small Molecule Kit

176-1030
ProteOn protocol development kit, includes carbonic anhydrase II/CBS pair, GLM sensor chip, amine coupling kit, 50 ml Na acetate buffer, pH 5.0

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Number Description Options
6295 ProteOn Sensor Chips, Tips and Techniques, Rev A Click to download
5679 Applications of the ProteOn GLH Sensor Chip: Interactions Between Proteins and Small Molecules, Rev A Click to download [ Add to Cart (Free) ]
5540 Screening, Ranking, and Epitope Mapping of Anti-Human IL-9 Supernatants, Rev A Click to download
5449 Applications of the ProteOn NLC Sensor Chip: Antibody-Antigen, DNA-Protein, and Protein-Protein Interactions, Rev B Click to download [ Add to Cart (Free) ]
5358 Mechanisms of Protein-Protein Binding: Double-Mutant Cycle Analysis Using the ProteOn XPR36 System, Rev A Click to download
5368 Anaylsis of Multiple Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev B Click to download
5367 Rapid Optimization of Immobilization and Binding Conditions for Kinetic Analysis of Protein-Protein Interactions Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download
5360 Rapid and Detailed Analysis of Multiple Antigen-Antibody Pairs Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download
3172 Rapid and Efficient Determination of Kinetic Rate Constants Using the ProteOn XPR36 Protein Interaction Array System, Rev B Click to download
5412 Antibody Characterization and Development: ProteOn XPR36 Protein Interaction Array System Application Guide, Rev C Click to download
5413 ProteOn XPR36 Hardware Product Information Sheet, Rev B Click to download
5410B ProteOn Protocol Development Kits Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5404 ProteOn Sensor Chips Product Information Sheet, Rev E Click to download [ Add to Cart (Free) ]
5538 Analyzing Protein Interactions With the ProteOn XPR36 Protein Interaction Array System, Rev D Click to download [ Add to Cart (Free) ]
5390 ProteOn XPR36 Protein Interaction Array System Brochure, Rev D Click to download [ Add to Cart (Free) ]
5409 Protein Interaction Analysis Ordering Information Sheet, Rev E Click to download [ Add to Cart (Free) ]
5434A ProteOn XPR36 Protein Interaction Array System Folder. (Contains bulletins 3172, 5358, 5360, 5367, 5368, 5390, 5404, 5409, 5410, 5413, 5538, and 5465 DVD, depending on availability) [ Add to Cart (Free) ]
6161 Highly Efficient Lipoparticle Capture and SPR Binding Kinetics of a Membrane Protein Using the ProteOn XPR36 Protein Interaction Array System, Rev A Click to download [ Add to Cart (Free) ]
6414 ProteOn XPR36 Experimental Design and Application Guide, Rev A Click to download