ProteoMiner Protein Enrichment Kits

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Overview

ProteoMiner Video Demonstration
ProteoMiner Technology

ProteoMiner technology. Each bead features a different hexapeptide ligand with affinity for specific proteins in a sample. Samples are applied to the beads, allowing proteins to bind to their specific ligands. Proteins in excess are washed away, and those proteins bound to the beads are eventually eluted, allowing further downstream analysis.

ProteoMiner Enrichment Kit

The ProteoMiner protein enrichment kit improves resolution and spot counts in 2-D gels. In an untreated sample, albumin and other high-abundance proteins dominate the gel, obscuring signals from less abundant proteins; on a gel generated using an equal amount of total protein from a treated serum sample, however, resolution is dramatically improved and a greater number of protein spots are visualized.

ProteoMiner protein enrichment technology employs a large, highly diverse bead-based library of combinatorial peptide ligands, which simultaneously dilutes high-abundance proteins and concentrates low-abundance proteins. When a complex biological sample is applied to the beads, high-abundance proteins saturate their high-affinity ligand(s) and excess protein is washed away. In contrast, low-abundance proteins are concentrated on their specific affinity ligand(s).

ProteoMiner protein enrichment kits:

  • Decrease the amount of high-abundance proteins without immunodepletion, preventing the loss of proteins bound to high-abundance proteins.
  • Enrich medium- and low-abundance proteins that cannot be detected through traditional methods.
  • Do not rely on a predefined set of antibodies, unlike immunodepletion products.
  • Are compatible with a variety of sample types.
  • Offer a convenient, easy-to-use format.
  • Can be used for differential expression analysis.

 

Multiple Kits Available
A small-capacity kit is available for processing ≥10 mg of protein, while a large-capacity kit is available for processing ≥50 mg of protein. These kits have been optimized for use with serum and plasma samples, but they may be used with other sample types within the recommended total protein range.


The small- and large-capacity kits are available in two formats:

  • ProteoMiner protein enrichment kits — provide all necessary reagents for accessing low-abundance proteins, utilizing a single elution reagent in a simple, easy-to-perform process.
  • ProteoMiner sequential elution kits — utilize multiple elution reagents to sequentially elute proteins based on different properties. These kits fractionate proteins to improve detection and resolution and have been optimized for SELDI experiment samples (reagents are not compatible for direct use with 2-D gel electrophoresis).

 

ProteoMiner Sequential Elution Reagents

163-3003
10 preps, protein preparation reagents for eluting proteins into multiple fractions, columns not included, for use with #163-3000

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ProteoMiner Protein Enrichment Small-Capacity Kit

163-3006
10 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

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ProteoMiner Protein Enrichment Large-Capacity Kit

163-3007
10 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

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ProteoMiner Protein Enrichment Introductory Small-Capacity Kit

163-3008
2 preps, low-abundance protein enrichment kit for 10 mg of total protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes

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ProteoMiner Protein Enrichment Introductory Large-Capacity Kit

163-3009
2 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes

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ProteoMiner Sequential Elution Small-Capacity Kit

163-3010
10 preps, protein preparation kit for sequential elution into 4 fractions, for 10 mg of total protein, includes 10 spin columns, buffers, collection tubes

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ProteoMiner Sequential Elution Large-Capacity Kit

163-3011
10 preps, protein preparation kit for sequential elution into 4 fractions, for 50 mg of total protein, includes 10 spin columns, buffers, collection tubes

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Number Description Options
5993 Comparative Proteomics of Umbilical Cord and Maternal Plasma, Rev A Click to download
5632 Accessing Low-Abundance Proteins in Serum and Plasma With a Novel, Simple Enrichment and Depletion Method, Rev B Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download [ Add to Cart (Free) ]
5635 ProteoMiner Protein Enrichment Technology Bulletin, Rev B Click to download
5835 ProteoMiner Sequential Elution Kit Protocol, Rev A Click to download
5841 Quantitation of Serum and Plasma Proteins After Enrichment of Low-Abundance Proteins With the ProteoMiner Protein Enrichment System, Rev A Click to download
10010636 Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D Click to download
10011083 ProteoMiner Protein Enrichment and Sequential Kit Protocols, Rev A Click to download
5916 Enrichment of Medium- and Low-abundance Proteins in Sample Types Using ProteoMiner Technology, Rev A. Click to download
6441 2-D Analysis of Leaf Protein Samples Treated with ProteoMiner Beads Under Denaturing and Nondenaturing Conditions Poster, Rev A Click to download