ProteinChip Antibody Capture Kit

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SELDI Citations
SELDI Citations Database
 

SELDI Citations Database

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Biomarker Discovery Using SELDI Technology

Proper study and experimental design coupled with the correct data analysis approach are critical components of successful biomarker discovery, no matter which technology, platform, or workflow is used. Bio-Rad has produced two in-depth guides to provide researchers with strategies for success.

The first guide, Biomarker Discovery Using SELDI Technology — A Guide to Successful Study and Experimental Design, provides a series of general guidelines for effective study design using virtually any proteomics technology, and gives specific recommendations for using the ProteinChip surface-enhanced laser desorption/ionization (SELDI) system. This guide also includes:

  • Guidelines for proper sample selection, collection, handling, and storage
  • Instructions for planning the experimental workflow
  • Tips on improving reproducibility through standard operating procedures
  • Important considerations for data analysis implementation
  • Optimized methods and protocols for serum or plasma analysis


The second guide, Biomarker Discovery Using SELDI Technology — A Guide to Data Processing and Analysis Using ProteinChip Data Manager Software, is a comprehensive resource for data analysis workflows, guidelines, and specific recommendations for analyzing biomarker discovery data. The guide covers:

  • General data analysis workflows
  • Data organization
  • Data processing steps, including baseline subtraction, normalization, and spectrum alignment
  • Statistical analysis using P values and ROC curves, hierarchical clustering, and principal component analysis
  • Glossary of software terms

The ProteinChip antibody capture kit enables the capture and differentiation of multiple antigens based on molecular weight using the ProteinChip SELDI system. It eliminates the laborious assay development and optimization steps typically required to evaluate antibody-antigen interactions.

Use the ProteinChip antibody capture kit to conduct epitope discovery experiments by first proteolytically cleaving proteins captured on ProteinChip arrays (on-chip proteolysis) and then using SELDI to highlight the peptide(s) associated with the epitope. This streamlines the process of sequencing the protein binding site via tandem mass spectrometry

ProteinChip Antibody Capture Kit

K10-00005
Kit contains antibody, antigen, reagents, controls, 6 ProteinChip PG20 arrays, and ProteinChip SPA matrix

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Number Description Options
5530 ProteinChip SELDI System Brochure, Rev A Click to download
5524 ProteinChip Arrays and Reagents Product Information Sheet, Rev C Click to download
10008252 Instruction Manual, ProteinChip Antibody Capture Kit, Rev D Click to download