Profinity eXact Purification Resin

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The Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose-based matrix. Resulting purity of the eluted protein is typically higher than that for other affinity-tag systems due to the specific recognition (KD < 100 pM) that subtilisin has for its prodomain sequence (Profinity eXact tag). Once the Profinity eXact fusion protein has been captured by the Profinity eXact purification resin — and the cleavage reaction has been triggered with a sodium fluoride-containing buffer — the affinity tag is precisely cleaved at the C-terminus of the cleavage recognition sequence (EEDKLFKAL). The result is a quickly purified protein product that lacks any amino acid residues derived from the Profinity eXact tag.

Benefits of Profinity eXact resin:

  • Purification and processing of fusion-tagged proteins in a single-step
  • On-column cleavage in as little as 30 min
  • No protease addition required
  • Precise cleavage at N-terminus to generate native protein sequence

The following convenient packaging configurations allow easy evaluation of Profinity eXact technology:

  • Profinity eXact resin — 10 ml resin supplied in 100 mM sodium phosphate, pH 7.2 containing 0.02% sodium azide
  • Profinity eXact mini spin columns — 10-pack of single-use columns that contain 0.1 ml settled resin in addition to all required collection tubes for binding, washing, and elution steps. Fit all benchtop centrifuges
  • Profinity eXact mini spin starter kit — comprehensive 10-pack kit that ensures purification success upon initial trial. Includes 10 prepacked spin columns, collection tubes, lysis reagent, buffers for purification, and lyophilized MBP control protein lysate
  • Bio-Scale Mini Profinity eXact cartridges — 1 ml and 5 ml cartridges that can be used with BioLogic chromatography systems, the Profinia protein purification system, a peristaltic pump, or with appropriate column adaptors with any other chromatography system
  • Profinity eXact expression and purification starter kit — includes two kits: the Profinity eXact cloning and expression starter kit for the cloning and expression of a target gene using pPAL vectors and competent cells, and the Profinity eXact mini spin purification starter kit for single-step purification and on-column cleavage of the tagged protein
Profinity eXact Purification Resin

156-3005
10 ml, purification resin supplied in 100 mM sodium phosphate, pH 7.2 with 0.02% sodium azide

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Profinity eXact Expression and Purification Starter Kit

156-3008
Kit includes 1 Profinity eXact cloning and expression starter kit (20 reactions) and 1 Profinity eXact mini spin purification starter kit (10 spin columns)

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Profinity eXact Mini Spin Purification Starter Kit

156-3006
Mini purification starter kit includes 10 prepacked spin columns, collection tubes, 1 vial lyophilized lysate, lysis reagent and purification buffers

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Number Description Options
5655 Profinity eXact Resin Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5668 Profinity eXact Fusion-Tag System Brochure, Rev A Click to download [ Add to Cart (Free) ]
5646 Profinity eXact Cloning and Expression Kits, PIS, Rev B Click to download [ Add to Cart (Free) ]
5656 Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System, Rev B Click to download [ Add to Cart (Free) ]
5652 Profinity eXact Fusion-Tag System Performs On-Column Cleavage and Yields Pure Native Protein From Lysate in Less Than an Hour, Rev A Click to download [ Add to Cart (Free) ]
10011163 Profinity eXact Mini Spin Columns Manual, Rev A Click to download
5766 Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A Click to download [ Add to Cart (Free) ]
5725 Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
10011260 Profinity eXact Protein Purification System Manual, Rev A Click to download [ Add to Cart (Free) ]