Precision Melt Analysis™ Software

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Overview

Precision Melt Analysis Software System Requirements
System Minimum Recommended
Operating system Windows XP Professional SP2, Windows 7 Home Premium Windows XP Professional SP2, Windows 7 Home Professional
Drive CD-ROM CD-RW
Hard drive 10 GB 20 GB
Processor speed 1 GHz 2 GHz
RAM 1 GB (2 GB for Windows 7) 2 GB
Screen resolution 1,024 x 768 with true-color mode 1,280 x 1,024 with true-color mode
USB port 2.0 Hi-Speed 2.0 Hi-Speed
Internet browser Internet Explorer Internet Explorer
Software   Microsoft Office Suite
SNP Genotyping
 

SNP genotyping by Precision Melt Analysis software using data generated by the CFX96 real-time PCR detection system. Discrimination of human factor V coagulation SNP genotypes (C to T substitution) using SsoFast EvaGreen supermix. Data from homozygous wild type (), mutant (), and heterozygote () samples are shown on a normalized melt curve plot. RFU, relative fluorescence units.

Difference Curve Plot
 

A representative difference curve plot generated from data normalized to a mutant sample. Data from homozygous wild type (), mutant (), and heterozygote () samples are shown. RFU, relative fluorescence units.

Melt Curve Plot
 

Multifile melt study analysis. Compare melt curves from different files using the Melt Study module in Precision Melt Analysis software. RFU, relative fluorescence units.

Precision Melt Analysis software imports and analyzes data files generated from all CFX real-time PCR detection systems to genotype samples based on the thermal denaturation properties of double-stranded DNA. The software can be used for a variety of genotyping applications, including scanning for new gene variants, screening DNA samples for SNPs, identifying insertions/deletions or other unknown mutations, and determining the percentage of methylated DNA in unknown samples. Use the default analysis settings to automatically normalize data and assign a genotype to each sample based on its melt characteristics — there is no need to include genotype controls to assign cluster labels.

Precision Melt Analysis software saves analysis time by assigning sample genotypes automatically based on cluster analysis, or manually using multiple data view options to tailor the software to the appropriate analysis. Use the normalized melt curves plot feature to generate a basic representation of the different clusters based on curve shifting (for homozygotes) and curve shape change (for heterozygotes). Difference curve plots of a sample fluorescence versus a selected control at each temperature transition provide a convenient visual aid to interpret the data.

Precision Melt Analysis software enables data comparison between multiple file runs by combining data into a single Melt Study. Develop a standard library of melt curve runs to analyze an unlimited number of melt experiments without having to export data.

Precision Melt Analysis software makes it easy for you to:

  • Streamline your data analysis using the customizable default analysis settings
  • Utilize the multiple data view options to manually assign sample genotypes by tailoring the software to the appropriate analysis
  • Examine results from a number of melt files, without having to export data, using the Melt Study module
  • Analyze multiple experiments from a single plate using the Well Groups feature
  • Publish your data in several formats by easily exporting data to Microsoft Excel or as an image

 

Precision Melt Analysis Software

184-5025
2 user licenses, data analysis software for all CFX real-time PCR detection systems, 2 HASP HL keys, calibration kit, installation CD

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Precision Melt Analysis Software Only

184-5015
2 user licenses, data analysis software for all CFX real-time PCR detection systems, 2 HASP HL keys, installation CD

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Melt Calibration Kit

184-5020
Kit includes melt calibration DNA standard, melt primers, instructions

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Number Description Options
6009 High Resolution Melt Parameter Considerations for Optimal Data Resolution, Rev A Click to download [ Add to Cart (Free) ]
6004 A Practical Guide to High Resolution Melt Analysis Genotyping, Rev A Click to download [ Add to Cart (Free) ]
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download [ Add to Cart (Free) ]
10014811 Instruction Manual, Precision Melt Analysis Software, Rev B Click to download
5798 Precision Melt Analysis Software Flier, Rev B Click to download [ Add to Cart (Free) ]
10014810 Instruction Manual, Precision Melt Analysis Software Quick Guide, Rev C Click to download
10015798 Instruction Manual, Melt Calibration Kit, Rev D Click to download