PowerPac™ HV High-Voltage Power Supply

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Overview

Power Supply Selection Guide
Technique and Recommended Apparatus Gel or Tube Size,* Qty (W x L x Thickness) Typical Conditions** (Initial) Typical Conditions** (Final) Run Time PowerPac™ Power Supply
W V mA W V mA
Laemmli (SDS), O'Farrell Second Dimension (SDS)
PROTEAN® II xi cell 160 x 160 x 1.5 mm, 2 gels 100 35(C) 350 35(C) 5 hr HV or Universal
PROTEAN II XL cell 183 x 200 x 1.5 mm, 2 gels 100 35(C) 350 35(C) 5 hr HV or Universal
Criterion™ cell 133 x 87 x 1.0 mm, 2 gels 200(C) 200 200(C) 80 55 min Basic or HC
Criterion™ 133 x 87 x 1.0 mm, 2 gels 300(C) 224 300(C) 164 20–26 min Basic, HV, or Universal
Mini-PROTEAN® Tetra cell 83 x 73 x 1.0 mm, 4 gels 200(C) 240 200(C) 120 35–45 min Basic or HC
Mini-PROTEAN® Tetra Cell 83 x 73 x 1.0 mm, 4 gels 300(C) 360 300(C) 228 15 min Basic, HV, or Universal
Mini-PROTEAN® Tetra Cell 83 x 73 x 1.0 mm, 4 gels 400(C) 456 400(C) 416 10 min HV or Universal
High-Throughput Electrophoresis
PROTEAN Plus 200 x 205 x 1.0 mm, 12 gels 200(C) 1,000 200(C) 350 6 hr HC or Universal
Dodeca™ cell 250 x 205 x 1.0 mm, 12 gels 200(C) 1,000 200(C) 350 6 hr HC or Universal
256 x 230 x 1.0 mm, 12 gels 150(C) 1,200 150(C) 300 18–20 hr HC or Universal
PROTEAN II xi/XL multi-cell 160 x 200 x 1.5 mm, 6 gels 150 480(C) 500 480(C) 5 hr Universal
Criterion™ Dodeca™ cell 133 x 87 x 1.0 mm, 12 gels 200(C) 1,000–1,400 200(C) 400–500 55 min HC or Universal
Mini-PROTEAN® 3 Dodeca™ cell 83 x 73 x 1.0 mm, 12 gels 200(C) 600 200(C) 360 45 min HC or Universal
IEF, O'Farrell First Dimension
PROTEAN II xi cells 150 x 1.5 mm tubes, 4 (minimum) 800(C) 3.5 800(C) <1 16 hr HV or Universal
Mini-PROTEAN II tube cell 75 x 1.0 mm tubes, 8 (minimum) 750(C) 1 750(C) <1 3–4 hr HV or Universal
Preparative Electrofocusing
Rotofor® cell 60 ml focusing chamber 15(C) 500 24 15(C) 1,200 10 4 hr HV
Mini Rotofor cell 18 ml focusing chamber 12(C) 500 24 12(C) 1,200 10 4 hr HV
MicroRotofor™ cell 2.5 ml focusing chamber 1(C) 100 10 1(C) 500 2 <3 hr HV or Universal
Preparative PAGE
Model 491 prep cell 10(C) 300 40 10(C) 400 30 3–8 hr HV or Universal
Mini prep cell 1(C) 200 5 1(C) 300 3 3–8 hr HV or Universal
Protein Electroelution
Model 422 electro-eluter 6 samples 200 60(C) 150 60(C) 3–4 hr Basic, HV, or Universal
Whole gel eluter 160 x 140 mm or larger 15 200(C) 15 200(C) 20 min Basic
Mini whole gel eluter 55 x 65 mm or larger 10 100(C) 10 100(C) 20 min Basic
Polyacrylamide Analytical Electrofocusing
Model 111 mini IEF cell 125 x 65 x 0.4 mm 100(C) 6 100(C) 4 15 min HV or Universal
200(C) 6 200(C) 4 15 min Basic
450(C) 4 450(C) 1 1 hr Basic
DNA Restriction Analysis (Horizontal Mode)
Sub-Cell® GT cell 150 x 200 x 5 mm 80(C) 55 80(C) 60 4 hr Basic or HC
Mini-Sub® cell GT cell 70 x 100 x 5 mm 50(C) 25 50(C) 30 2 hr Basic
Wide Mini-Sub cell GT cell 150 x 100 x 5 mm 50(C) 35 50(C) 40 2 hr Basic
DNA Sequencing
Sequi-Gen® GT system 380 x 500 x 0.4 mm 80(C) 1,850 30 80(C) 1,850 30 2–4 hr HV
SSCP
Sequi-Gen GT system 210 x 400 x 0.4 mm 40(C) 1,800 20 40(C) 1,800 20 2–3 hr HV
Microsatellite Mapping
Sequi-Gen GT system 210 x 400 x 0.4 mm 50(C) 2,100 25 50(C) 2,100 25 2–3 hr HV
Mutation Detection
DCode™ system 100 x 75 x 1.0 mm, 2 gels 130(C) 130(C) 2.5 hr Basic or HC
Western Blotting
Mini Trans-Blot® cell 83 x 73 x 0.75 mm, 2 gels 100(C) 250 100(C) 450 1 hr HC
Criterion blotter
  Wire electrodes 133 x 87 x 1.0 mm, 2 gels 100(C) 250 100(C) 450 1 hr HC
  Plate electrodes 133 x 87 x 1.0 mm, 2 gels 100(C) 650 100(C) 1,600 30 min HC
Trans-Blot® cell
  Wire electrodes 200 x 160 x 1.5 mm, 1 gel 60(C) 210–250 60(C) 210—250 5 hr HC
  Plate electrodes 200 x 160 x 1.5 mm, 1 gel 100–150(C) 1,000–1,600 100–150(C) 1,000–1,600 1–5 hr HC
  High-intensity transfer 200 x 160 x 1.5 mm, 1 gel 50–100(C) 1,600 50–100(C) 1,600 30 min HC
Trans-Blot Plus cell 265 x 280 x 1.5 mm, 3 gels 100(C) 3,000 100(C) 3,000 30 min–1 hr HC
Semi-Dry Blotting
Trans-Blot SD cell
  Protein 250 x 180 x 1.5 mm 15(C) 500 15(C) 200 15–30 min HC
  DNA/RNA 150 x 150 x 6 mm 15 650(C) 25 650(C) 10–30 min HC
 

* Sizes shown are typical for the corresponding apparatus. For running conditions for additional sizes, see the product instruction manuals.

** (C) = constant. Typical conditions are listed as guidelines only and can vary based on sample, buffers, etc.

PowerPac™ Power Supply Comparison
  PowerPac Basic PowerPac HC PowerPac Universal PowerPac HV
Output range (programmable)
  Volts 10–300 V 5–250 V 10–500 V 20–5,000 V
  Current 4–400 mA 0.01–3.0 A 0.01–2.5 A 0.01–500 mA
  Power 75 W (maximum) 1–300 W 1–500 W 1–400 W
Type of output (with automatic crossover) Constant voltage or constant current Constant voltage, constant current, or constant power Constant voltage, constant current, or constant power Constant voltage, constant current, constant power, or constant temperature
Timer 1–999 min 1 min–99 hr, 59 min 1 min–99 hr, 59 min 1 min–99 hr, 59 min
Volt-hour control No No Yes, 99,000 V-hr Yes, 99,000 V-hr
Pause/resume function Yes Yes Yes Yes
Display 3-digit LED 16-character x 2-line LCD 128 x 64 pixel, backlit 128 x 64 pixel, backlit
Programmable methods 1 method up to 3 steps, no storage capability Stores 9 methods, each with up to 9 steps Stores 9 methods, each with up to 9 steps
Real-time clock No No Yes Yes
Automatic recovery after power failure Yes Yes Yes Yes
Data transfer/archiving No No Yes, optional Yes
Temperature control No No No Via temperature probe; 30–90°C ± 2°C
Microampere readout No No No Yes
Safety features No-load detection; sudden load change detection, overload/short-circuit detection; overvoltage protection No-load detection; sudden load change detection; groundleak detection, overload/short-circuit detection; overvoltage protection No-load detection; sudden load change detection; groundleak detection, overload/short-circuit detection; overvoltage protection No-load detection; sudden load change detection; ground leak detection, arc detection;overload/short-circuit detection; overvoltage protection
Operating conditions 0–40°C; 0–95% humidity 0–40°C; 0–95% humidity 0–40°C; 0–95% humidity 0–40°C; 0–95% humidity
Stackable Yes Yes Yes Yes
Number of output jacks 4 sets in parallel 4 sets in parallel 4 sets in parallel 4 sets in parallel
Regulatory EN-61010, CE EN-61010, CE EN-61010, CE EN-61010, CE
IQ/OQ protocols No No Yes, optional Yes, optional
Input power (actual) 90–120 or 198–264 VAC, 50/60 Hz, autoswitching 90–120 or 198–264 VAC, 50/60 Hz, autoswitching 90–120 or 198–264 VAC,50/60 Hz, autoswitching 90–120 or 198–264 VAC,50/60 Hz, autoswitching
Dimensions (W x D x H) 21 x 24.5 x 6.5 cm 25 x 28.5 x 8 cm 27.5 x 34 x 10 cm 27.5 x 34 x 10 cm
Weight 1.1 kg (2.4 lb) 2.0 kg (4.4 lb) 2.5 kg (5.5 lb) 2.85 kg (6.3 lb)

The PowerPac™ HV power supply supports an output of 5,000 V, 500 mA, and 400 W, which allows its use for all high-voltage applications, including low-current applications in the microampere range. It is ideal for IEF and DNA sequencing. With 400 W output, the PowerPac HV offers enough power to run the most demanding IEF experiments or up to four DNA sequencing cells simultaneously.

Features and Benefits

Innovative, wireless run data transfer is possible with optional PowerPac data transfer software, which makes it easy to organize, display, print, analyze, export, and annotate run data from the PowerPac HV power supply. Easily transfer run data from anywhere in the laboratory using the power supply's built-in wireless IR capability. Data can be sent first to a PDA and then to a PC or directly to a PC with a peripheral IR receiving device.

PowerPac data transfer software allows you to:

  • Examine and track the programmed method
  • Examine actual run conditions (voltage, current, method changes, error conditions) recorded periodically during the run
  • Annotate a run with user notes
  • Identify the serial number of the unit that generated the data
  • Document all run-related information with reports
  • Organize your run data into projects
  • Customize data representation

IQ/OQ

To support IQ/OQ within GLP- and FDA-regulated environments, the PowerPac HV IQ/OQ protocol and test box are available for use exclusively with the PowerPac HV power supply. The test box houses a factory-calibrated resistive load. When used with the appropriate meters to monitor output voltage, current, and power, the test box determines the power supply's performance against its specifications.

Temperature Probe

The optional temperature probe is used with the PowerPac HV power supply to monitor gel temperature between 30 and 90°C during electrophoresis. Temperature control is particularly useful for DNA sequencing and SSCP analysis, in which constant temperature is important for resolution and reproducibility.

Applications and Uses

  • Sequencing
  • Nucleic acid gel electrophoresis
  • Protein gel electrophoresis

To select the power supply that best fits your needs, see the Power Supply Selection Guide or the PowerPac Power Supply Comparison chart.

 

Output (programmable) Voltage 20–5,000 V
Current 0.01–500 mA
Power 1–400 W
Type of output Constant voltage, constant current, constant power, or constant temperature (with automatic crossover)
Timer control 1 min–99 hr 59 min, fully adjustable
Volt-hour control Yes, 99,999 V-hr
Pause/resume function Yes
Display functions 128 x 64 pixel, yellow-green backlit graphics LCD
Programmable methods Stores up to 9 basic and 9 IEF methods, each with up to 9 steps
Real-time editing Yes
Real-time clock Yes
Automatic recovery after power failure Yes, user-selectable; setup values maintained
Data transfer/archiving Yes
Temperature control Yes, via temperature probe; 30–90°C ±2°C
Microampere readout and control Yes
Safety features No-load detection, sudden load change detection, ground leak detection, overload/short circuit protection, overvoltage protection
Operating conditions 0–40°C, 0–90% humidity in the absence of condensation
Stackable Yes
Number of output jacks 4 sets in parallel
Regulatory EN-61010, CE
IQ/OQ protocols Yes
Input power (actual) 90–120 or 198–264 VAC, 50/60 Hz, autoswitching
Dimensions (W x L x H), cm/in 7.5 x 34 x 10/3 x 13.4 x 3.9
Weight, kg/lb 2.85/6.3
PowerPac HV Power Supply #164-5056
PowerPac HV Power Supply

164-5056
100–120/220–240 V, power supply for high-voltage applications including low-current applications such as IEF and DNA sequencing, includes power cord

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PowerPac HV Power Supply With Temperature Probe #164-5059
PowerPac HV Power Supply with Temperature Probe

164-5059
100–120/220–240 V, high-voltage power supply, includes power cord, temperature probe for monitoring gel temperature (30-90°C)

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PowerPac™ Data Transfer Software

164-5097
1-user license, PC, version 2.0, software, for organizing, analyzing, and annotating run data from PowerPac Universal and Power Pac HV Power Supplies

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PowerPac HV IQ/OQ Protocol Binder and Test Box #164-5098
PowerPac HV IQ/OQ Protocol Binder and Test Box

164-5098
Pkg of 1, set of protocol binder and test box, to support IQ/OQ within GLP- and FDA- regulated environments

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PowerPac HV IQ/OQ Protocol Binder #164-5099
PowerPac HV IQ/OQ Protocol Binder

164-5099
Pkg of 1, protocol binder, to support IQ/OQ within GLP- and FDA- regulated environments

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Number Description Options
6371 Electrophoresis Power Supplies Brochure, Rev C Click to download
4006012 Instruction Manual, PowerPac Temperature Probe, Rev C Click to download
10002468 Instruction Manual, PowerPac HV Power Supply, Rev D Click to download