iQ™5 Optical System Software, Version 2.1



Computer Specifications

To ensure optimal performance of the real-time PCR detection system and software for analysis or data collection, load the software on a computer with the following specifications:

Recommended Computer Specifications
CPU Pentium or comparable processor, 2.0 GHz or faster
Operating system Windows XP or Windows Vista
Hard drive 20 GB
Minimum screen resolution 1,024 x 768 x 256 colors
Hi-Speed communication ports At least 4 USB 2.0
Other hardware CD-RW drive; mouse
Software Functionality

Intuitive Plate and Protocol Setup for Single or Multiplex Experiments

Import sample identifiers such as a tissue or gene description or a sample number. Save your commonly used plates and protocols to quickly start running experiments. Create gradient and melt protocols for rapid assay optimization. Sample type and concentration values are editable even after run completion, ensuring that errors in data entry do not affect results.

Advanced Analysis and Reporting Functions

Powerful quantitative analysis tools. Display multiple standard curves and compare reaction efficiencies. Personalize trace styles for easy visual comparisons.

Powerful gene expression analysis for single or multiple run files. Analyze up to 5,000 threshold cycle (CT) values from multiple real-time PCR data files. Incorporate multiple reference genes and account for differences in reaction efficiencies.

Comprehensive sortable data analysis reports. Export data from tables with one-step Export to Excel. Copy or print options for graphs and tables. Create customizable .pdf reports.

Additional Analysis Tools

Melt curve plot and melt curve analysis.

Probe-based allelic discrimination. Select Threshold Cycle mode or RFU mode to initiate the automatic calling of sample types. All sample type assignments are easily editable.

qPCR Applications Guide
qPCR Applications Guide

The qPCR Applications Guide is a comprehensive source of information on real-time PCR. Complete with detailed information on optimizing, validating, and analyzing real-time PCR data, this technically focused applications guide is a resource for experienced researchers as well as those wanting to learn more about using real-time PCR for applications such as qPCR, RT-qPCR, genotyping, and GMO detection. This guidebook includes chapters that cover:

  • Key concepts of real-time PCR
  • Getting started — experimental design
  • Multiplexing considerations
  • Data analysis
  • Gene expression analysis
  • Genotyping allelic discrimination
  • GMO detection

This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization, and data analysis of qPCR experiments.

iQ5 optical system software, version 2.1, supports the collection and analysis of real-time PCR data from the iQ5, redesigned MyiQ, and MyiQ2 real-time PCR detection systems. The software can also analyze data files generated by earlier versions of iCycler iQ, iQ5, and MyiQ software packages.

Features include:

  • Advanced gene expression analysis by relative quantity (ΔCT) or normalized expression (ΔΔCT), accounting for differences in reaction efficiency or use of multiple reference genes
  • Gene study feature enables direct comparison of gene expression results from up to 5,000 CT data points and multiple plates
  • Flexible allelic discrimination analysis tool allows automatic sample identification in genotyping experiments
  • Multiple data reporting options to generate publication-quality statistics and graphs, quickly create customizable reports, or export data from tables with 1-step Export to Excel
  • User preference settings for managing data files, analysis settings, and access privileges


Number Description Options
10005604 Instruction Manual, iQ5 Optical System Software, Rev C Click to download
5279 Real-Time PCR Applications Guide, Rev B Click to download