iQ™5 Optical System Software, Security Edition



qPCR Applications Guide
qPCR Applications Guide

The qPCR Applications Guide is a comprehensive source of information on real-time PCR. Complete with detailed information on optimizing, validating, and analyzing real-time PCR data, this technically focused applications guide is a resource for experienced researchers as well as those wanting to learn more about using real-time PCR for applications such as qPCR, RT-qPCR, genotyping, and GMO detection. This guidebook includes chapters that cover:

  • Key concepts of real-time PCR
  • Getting started — experimental design
  • Multiplexing considerations
  • Data analysis
  • Gene expression analysis
  • Genotyping allelic discrimination
  • GMO detection

This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization, and data analysis of qPCR experiments.

iQ5 optical system software, version 2.0, supports the collection and analysis of real-time PCR data from both the iQ5 and the redesigned MyiQ real-time PCR detection systems. The software can also analyze data files generated by earlier versions of MyiQ, iCycler iQ, and iQ5 software packages.

In addition to the features of the standard version, the Security Edition of iQ5 optical system software works with the security features of Microsoft Windows XP to integrate GLP standards for data collection and analysis. The Security Edition is specifically designed for scientists operating in regulated environments, providing embedded tools for compliance with U.S. FDA 21 CFR Part 11 regulations.

Key Features

  • User log-in with optional password protection
  • Preset user roles with customizable levels of access to software features
  • User preference settings for managing data files and analysis settings
  • Gene study feature for direct comparison of gene expression results from up to 5,000 CT data points and multiple plates
  • Windows-based password protection for secure user log-in and file access
  • Electronic signatures that can be applied to all calibration and data files
  • Detailed audit trail and event-log tracking to support compliance with electronic record-keeping regulations
  • Reports generated in PDF format to maintain integrity of data files generated with the software
Recommended Computer Specifications  
To ensure optimal performance of the real-time PCR detection system and software for analysis or data collection, load the software on a computer with the following specifications:  
CPU Pentium or comparable processor, 2.0 GHz or faster
Operating system Windows XP
Hard drive 20 GB
Minimum screen resolution 1,024 x 768 x 256 colors
Hi-Speed communication ports At least 4 USB 2.0
Other hardware CD-RW drive
Number Description Options
5593 iQ5 Optical System Software Security Edition (Version 2.0) Product Information Sheet, Rev A Click to download
5279 Real-Time PCR Applications Guide, Rev B Click to download