Criterion Stain Free Gel Imaging System

Print

Overview

Criterion™ Tris-HCI Gels
Precision Plus Protein Standards
Imaging System Selection Guide
  ChemiDoc™ MP GelDoc™ EZ GS-900 Gel Doc XR+ and ChemicDoc XRS+ PharosFX™, PharosFX Plus, Personal Molecular Imager
ChemiDoc™
MP
GelDoc™
EZ
GS-900™ Gel Doc
XR+
ChemiDoc
XRS+
PharosFX™ PharosFX Plus Personal Molecular Imager™
Nucleic Acid Detection
Ethidium bromide 5 4 - 4 4 5 5 -
SYBR® Green I stain 5 4 - 4 4 5 5 -
SYBR® Safe stain 5 4 - 4 4 5 5 -
Coumarin stain 5 4 5 - - - - -
Fast Blast™ DNA stain 4 4 5 4 4 - - -
Protein Staining, 1-D Gels
Stain-free gels 5 5 - 5 5 - - -
Coomassie Blue stain 4 4 5 4 4 2 2 -
Silver stain 4 4 5 4 4 2 2 -
SYPRO Ruby protein blot stain 4 4 - 4 4 5 5 -
Flamingo™ fluorescent gel stain 4 3 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 4 - 5 5 - - -
Protein Staining, 2-D Gels
Coomassie Blue stain 4 3 5 3 3 2 2 -
Silver stain 4 3 5 3 3 2 2 -
SYPRO Ruby protein blot stain 4 3 - 3 3 5 5 -
Flamingo™ fluorescent gel stain 4 2 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 3 3 3 3 3 3 3
Pro-Q stain 4 2 - 2 3 5 5 -
Cy2, Cy3, Cy5 label 4 - - - - 5 5 -
Blot Detection
Stain-free blots 4 5 - 4 5 - - -
Colorimetric blots 3 3 5 3 3 2 2 2
Coomassie Blue stain 4 - 5 4 4 2 2 -
Silver stain 5 - 5 4 4 2 2 -
SYPRO Ruby protein blot stain* 5 - - - - 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Chemifluorescence* 5 - - 1 1 4 4 -
Qdot* 5 2 - 2 2 5 5 -
Multiplex fluorescence 5 - - - - - - -
Micro- and Macroarray** Detection
Radiolabel - - - - - - 5 5
Fluorescence 5 - - 2 2 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Colony Counting
Colorimetric detection 5 - - 4 4 3 3 -
Fluorescent detection 5 - - 4 4 5 5 -
Isotopic
Radiolabel - - - - - - 5 5
X-ray film 4 - 5 4 4 3 3 -

- Not recommended; 1–5, recommendation level (5 = highest).
*    Optimal with low fluorescence PVDF membrane.
**   With spot features ≥400 µm.

The Criterion Stain Free™ gel imaging system allows direct visualization, analysis, and documentation of protein samples in SDS-PAGE gels with the single touch of a button, without staining, destaining, or gel drying procedures. The complete system is composed of the Criterion Stain Free imager*, Criterion Stain Free gels, and Image Lab™ software. This stain-free system is ideal for quick sample assessment prior to purification procedures. The system is also useful as a precursor to blotting and profiling workflows in which Coomassie Blue staining is ordinarily used as a detection method for purity assessment, quantitation, protein characterization (western blotting), and profiling (MALDI-TOF, LC-MS/MS). Benefits include:

  • Fast results — Elimination of staining, destaining, and gel drying steps shortens the electrophoresis portion of the experimental workflow, such as sample assessment and data analysis in protein purification; researchers can plan for the next purification step or for the next project
  • Reproducibility — Gel imaging and analysis is automated, enabling reproducible results. There is no background variability within a gel or between gels (as is often seen with standard Coomassie Blue staining); the Criterion Stain Free system enables standardization of methods
  • Green technology — Acetic acid and methanol are eliminated, reducing organic waste
  • Ease of use — One-touch gel image analysis processing and data reporting is accomplished through a single user interface, reducing the number of steps to achieve results

*The Criterion Stain Free imager is no longer offered by Bio-Rad Laboratories. It has been replaced by the new Gel Doc EZ imager. However, we provide support for those who have already purchased the system in case of any problems or questions. Click here to learn more about the new system.

Gel dimensions (W x L) 13.3 x 8.7 cm
Gel cassette dimensions (W x L) 15 x 10.6 cm
Gel storage conditions 4°C
Imager dimensions (W x L x H) 26 x 44 x 38 cm
Imager weight (W x L x H) 9.1 kg (20 lb)
Image Lab™ software OS compatibility Windows XP, Vista; Mac OS X
Number Description Options
5888 Bio-Rad Imaging Systems Family Brochure, Rev E Click to download [ Add to Cart (Free) ]
5782 In-Gel Protein Quantitation Using the Criterion Stain Free Gel Imaging System, Rev B Click to download
5793 Criterion Stain Free gel imaging system brochure, Rev A Click to download
5939 Overcoming the Coomassie Blues, Rev A Click to download
6126 Imaging Systems, Computer Specifications, Rev D Click to download
10014472 Instruction Manual, Criterion Stain Free Imaging System, Rev A Click to download