Gel Doc™ XR+ System

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Gel Doc XR+ System – Gel Imaging Systems

Overview

DNA Stains
DNA Stains

Bio-Rad offers a wide selection of products for a variety of gel detection applications. For nucleic acid detection, fluorescent stains are available for both DNA and RNA detection.

Precast Agarose Gels

ReadyAgarose™ gels are individually packaged precast agarose gels. The UV-transparent tray backing each ReadyAgarose gel locks into the Mini-Sub Cell GT and ReadySub-Cell GT electrophoresis chambers. These gels provide resolution of nucleic acids from 20 to 10,000 base pairs.

Precast Agarose Gels

Precast Agarose Gel Features

  •  
    • UV-transparent tray with fluorescent lane numbers and ruler
    • Tray locks into Bio-Rad electrophoresis cell of corresponding size
    • Mini 7 x 10 cm and wide mini 15 x 10 cm formats
    • Made with TAE or TBE buffer
    • Gel percentages for optimal resolution:
      1.0%, 200 bp–10 kb
      3.0%, 20 bp–1 kb
    • Shipment and storage at room temperature
    • One-year shelf life
DNA Electrophoresis Size Standards

EZ Load standards for DNA. Bio-Rad molecular rulers are available in several convenient size ranges and increments for sizing single- and double-stranded DNA. Visually distinct references bands make it easy to determine the size of your sample DNA.

Imaging System Selection Guide
  ChemiDoc™ MP GelDoc™ EZ GS-900 Gel Doc XR+ and ChemicDoc XRS+ PharosFX™, PharosFX Plus, Personal Molecular Imager
ChemiDoc™
MP
GelDoc™
EZ
GS-900™ Gel Doc
XR+
ChemiDoc
XRS+
PharosFX™ PharosFX Plus Personal Molecular Imager™
Nucleic Acid Detection
Ethidium bromide 5 4 - 4 4 5 5 -
SYBR® Green I stain 5 4 - 4 4 5 5 -
SYBR® Safe stain 5 4 - 4 4 5 5 -
Coumarin stain 5 4 5 - - - - -
Fast Blast™ DNA stain 4 4 5 4 4 - - -
Protein Staining, 1-D Gels
Stain-free gels 5 5 - 5 5 - - -
Coomassie Blue stain 4 4 5 4 4 2 2 -
Silver stain 4 4 5 4 4 2 2 -
SYPRO Ruby protein blot stain 4 4 - 4 4 5 5 -
Flamingo™ fluorescent gel stain 4 3 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 4 - 5 5 - - -
Protein Staining, 2-D Gels
Coomassie Blue stain 4 3 5 3 3 2 2 -
Silver stain 4 3 5 3 3 2 2 -
SYPRO Ruby protein blot stain 4 3 - 3 3 5 5 -
Flamingo™ fluorescent gel stain 4 2 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 3 3 3 3 3 3 3
Pro-Q stain 4 2 - 2 3 5 5 -
Cy2, Cy3, Cy5 label 4 - - - - 5 5 -
Blot Detection
Stain-free blots 4 5 - 4 5 - - -
Colorimetric blots 3 3 5 3 3 2 2 2
Coomassie Blue stain 4 - 5 4 4 2 2 -
Silver stain 5 - 5 4 4 2 2 -
SYPRO Ruby protein blot stain* 5 - - - - 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Chemifluorescence* 5 - - 1 1 4 4 -
Qdot* 5 2 - 2 2 5 5 -
Multiplex fluorescence 5 - - - - - - -
Micro- and Macroarray** Detection
Radiolabel - - - - - - 5 5
Fluorescence 5 - - 2 2 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Colony Counting
Colorimetric detection 5 - - 4 4 3 3 -
Fluorescent detection 5 - - 4 4 5 5 -
Isotopic
Radiolabel - - - - - - 5 5
X-ray film 4 - 5 4 4 3 3 -

- Not recommended; 1–5, recommendation level (5 = highest).
*    Optimal with low fluorescence PVDF membrane.
**   With spot features ≥400 µm.

The Gel Doc XR+ system is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence and densitometry. The system is controlled by Image Lab™ software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.

The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose™ gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.

Key benefits include:

  • Gel and blot imaging and analysis are quick and accurate
  • Automated, hands-off routines; no training is required
  • Save and recall all the steps in the workflow for repeatable and reproducible results
  • Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
  • Wide range of applications with special accessories to preserve sample integrity for downstream research while ensuring user safety
  • Comprehensive, automated quantitative analysis of protein and DNA samples in seconds
  • Customize and organize data in reports
  • Obtain publication-quality results quickly

The Gel Doc XR+ system optimizes reproducibility and reliability of experimental data, enabling quantitative comparisons between different experiments. Using proprietary algorithms, this imaging system is calibrated at setup to ensure:

  • Images are in focus at all times, regardless of zoom level or sample position
  • Appropriate flat fielding correction is automatically and consistently applied to image data for every application
  • Imaging artifacts are automatically corrected

The Gel Doc XR+ system enables quick and easy visualization, documentation, and analysis of nucleic acid and protein gels, blots, and macroarrays with a few clicks of the mouse. The system supports fluorescence and colorimetric detection methods. The Gel Doc XR+ system consists of a darkroom hood, CCD camera and software-controlled motorized lens, UV and white light illuminators, filter slider with standard filter, and UV-protection shield. The system enables you to:

  • Increase cloning efficiency and protein production by protecting DNA electrophoresis samples from UV exposure using the XcitaBlue™ conversion screen and blue light excitable stains such as GelGreen, SYBR® Safe, and SYBR® Green I
  • Maintain standard operating procedures or criteria for sample performance as there is no loss in sensitivity compared to UV and ethidium bromide staining

The Gel Doc XR+ system can be upgraded to the ChemiDoc XRS+ system.

Applications  
Chemiluminescence No
Fluorescence* Yes
Colorimetry/densitometry Yes
Gel documentation Yes
Hardware Specifications  
Maximum sample size 28 x 36 cm
Maximum image area 19.4 x 26 cm
Excitation source Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen and XcitaBlue™ UV/blue conversion screen available
Illumination control 3 modes (trans-UV, trans white, epi-white)
Detector CCD
Image resolution 4 megapixels
Pixel size (H x V) 4.65 x 4.65 µm
Filter holder 3 positions (2 for filters, 1 without filter)
Emission filters 1 included (standard), 3 optional
Dynamic range >3.0 orders of magnitude
Pixel density (gray levels) 4,096
Dynamic flat fielding Application specific, for all applications
Instrument size (L x W x H) 36 x 60 x 96 cm
Instrument weight 32 kg
Operating Ranges  
Operating voltage 110/115/230 V AC nominal
Operating temperature 10–28°C (21°C recommended)
Operating humidity <70% noncondensing
Automation Capabilities  
Workflow automated selection Application driven; user selected or recalled by a protocol
Workflow automated execution Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
Workflow reproducibility 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
Autofocus (patent pending) Precalibrated focus for any zoom setting or sample height
Image flat fielding** Dynamic; precalibrated and optimized per application
Autoexposure 2 user-defined modes (intense or faint bands)
*Use the optional XcitaBlue kit if performing SYBR® Safe DNA applications, because the UV to blue conversion screen allows you to visualize DNA samples while protecting against UV damage.

**U. S. patent 5,951,838.
 
Gel Doc™ XR+ System with Image Lab™ Software #170-8195
Molecular Imager® Gel Doc™ XR+ System with Image Lab™ Software

170-8195
Gel imaging system, includes darkroom, UV transilluminator, epi-white illumination, camera, cables, Image Lab software compatible with Windows or Mac OS

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Number Description Options
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