GAPDH PCR Module

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GAPDH PCR Module - Cloning and Sequencing Explorer Series

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 12 student workstations.
Initial GAPDH PCR primers, 50 µl 1
Nested GAPDH PCR primers, 50 µl 1
PCR master mix, 1.2 ml 3
Plasmid control DNA, 1 ml 1
Control Arabidopsis genomic DNA 1
Exonuclease I, 50 µl 1
Molecular weight ruler 1
Orange G loading dye, 1 ml 2
Sterile water 2.5 ml 1
PCR tubes, 0.2 ml 150
Capless PCR tube adaptors, 1.5 ml 150
Colored micro test tubes, 2.0 ml 120
Foam micro test tubes holders 12
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required accessories not included in kit:
Adjustable micropipets
    2–20 µl 1–12
    20–200 µl 1
Pipet tips, aerosol barrier
    2–20 µl, Xcluda B 1 box
    20–200 µl, Xcluda D 1 box
Thermal cycler 1
Recommended (optional) accessories:
Novel plant genomic DNA
Microcentrifuges
Vortexer
Cloning and Sequencing Explorer Series Overview

Prepare your students for the real world of scientific research. Engage them with the opportunity to perform novel, relevant research that can actually contribute to scientific knowledge. In this unique modular lab series, students are guided through an innovative research workflow identical to those performed in genomics labs worldwide. Over a multiple-week lab course, students will combine traditional and cutting edge molecular biology techniques and bioinformatics to clone, sequence, and analyze a housekeeping gene from a plant of your choice ensuring each class produces unique and novel data. The real thing. As a research and diagnostics manufacturing company, Bio-Rad brings unique strengths to the education market, including credibility, high quality products (kits and equipment that work), novel inquiry-based curricula, cutting edge kit applications, competitive pricing, superior teacher professional development programs, long-term strategic partners and allies, and strong personal endorsements from customers across all levels of education.

Conventional PCR Animation

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The GAPDH PCR module is an integral component of the Cloning and Sequencing Explorer Series. You can either use this module in conjunction with the electrophoresis module to visualize PCR results or purchase the module separately as a shorter stand-alone PCR laboratory activity to demonstrate applications of PCR in real research.

The experimental goal of the GAPDH PCR module is to amplify a portion of the GAPC gene, a housekeeping gene in the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) family, from a plant. After choosing a plant, students will amplify genomic and control plasmid DNA using two rounds of PCR from Arabidopsis (provided). Students may also amplify genomic DNA from two other plants, and additional genomic DNA may be extracted using the nucleic acid extraction module. Using degenerate primer sequences and multiple (nested) rounds of PCR, students will learn that amplifying novel DNA sequences can overcome the problems of the degradation of genetic code.

Students can analyze their PCR products using agarose gel electrophoresis and staining with ethidium bromide to visualize the GAPC gene they have isolated from the plant genome using the electrophoresis module.

Kit contains sufficient materials for 12 student workstations.  
Initial GAPDH PCR primers, 50 µl 1
Nested GAPDH PCR primers, 50 µl 1
PCR master mix, 1.2 ml 3
Plasmid control DNA, 1 ml 1
Control Arabidopsis genomic DNA 1
Exonuclease I, 50 µl 1
Molecular weight ruler 1
Orange G loading dye, 1 ml 2
Sterile water, 2.5 ml 1
PCR tubes, 0.2 ml 150
Capless PCR tube adaptors, 1.5 ml 150
Colored micro test tubes, 2.0 ml 120
Foam micro test tube holders 12
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
GAPDH PCR Module #166-5010EDU
GAPDH PCR Module

166-5010EDU
Second module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, PCR master mix, test tubes/holders, curriculum

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