Forensic DNA Fingerprinting Kit

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Overview

Purchasing Guide Table

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Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
DNA size standard
(HindIII lambda DNA digest), 100 µl
1
DNA samples, lyophilized, 60 µg each  
    Crime scene sample
    Suspect samples
1
5
EcoRI/PstI restriction enzyme mix, lyophilized, 3,000 units 1
Sample loading buffer, 5x, 1 ml 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Sterile water, 2.5 ml 1
Agarose powder, 5 g 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 80
Microcentrifuge tubes, 1.5 ml 30
Foam floats 8
Gel staining trays 4
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Horizontal gel electorphoresis chambers 4–8
Adjustable micropipets
    2–20 µl 1–8
    20–200 µl 1
    100–1,000 µl 1
Pipet tips
    2–200 µl, BR-35 1 bag
    100–1,000 µl, BR-40 1 bag
Power supplies 2–4
Recommended (Optional) Accessories:
Water bath or dry bath
 
Mini centrifuge  
Rocking platform  
Gel support film  
Microwave oven  
Forensic DNA Fingerprinting Kit Flowchart
Forensic DNA Fingerprinting Kit Flowchart
Key Kit Features
  • Aligns with AP Biology Lab 6
  • Study DNA restriction enzyme function
  • Use electrophoresis to separate DNA fragments
  • Construct standard curves from student data
  • Make precise determinations of DNA fragment sizes
  • Complete in two 45 minute lab sessions
Forensic DNA Fingerprinting Kit Curriculum Fit
Restriction Enzymes and Plasmid Mapping

Restriction enzymes are essential tools for molecular cloning and the mapping of genes and genomes. They are also used in genetic engineering to create recombinant DNA molecules for transforming bacterial, plant, or animal cells. Restriction enzymes recognize specific double-stranded DNA sequences and cut by making two incisions, one through each of the phosphate backbones of the double helix. The chemical bonds that the enzymes cleave are easily reformed by DNA ligases, so that restriction fragments carved from the DNA of different organisms can be spliced back together, creating new hybrid organisms.

 

In this lab, students observe the effects of two DNA restriction enzymes on a series of plasmid DNA samples. With the curriculum in this kit, students also have the opportunity to read plasmid maps and predict the sizes of DNA fragments from restriction enzyme digests prior to performing the lab. They can go one step further and use restriction digest maps of lambda bacteriophage genomes (provided in the kit curriculum) to design novel plasmids. In the process of doing these extension activities, students learn how restriction enzymes function and how they are used in genetic engineering. Use this kit to open the door to rich discussions about the scientific, ethical, and legal implications of forensics, DNA profiling, and genetic engineering.

Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.


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Complete Purchasing Guide

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Bulletin 5872
PowerPoint Presentation for Workshops and for Teaching

DNA Fingerprinting Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Forensic DNA Fingerprinting Kit and includes instructions for using the kit.

DNA Fingerprinting Kit Download PowerPoint presentation
DNA Fingerprinting Kit

DNA evidence assists in criminal, missing persons, mass disaster, and paternity cases. It can be used to identify a perpetrator or exonerate the innocent. Using real DNA as evidence, your students play the role of crime scene investigator to figure out for themselves "Who done it?"

The six DNA samples in the Forensic DNA Fingerprinting Kit are plasmids engineered to mimic the natural variations in DNA that exist between one human being and another. One DNA sample has been collected from a "crime scene" and five samples have been obtained from various "suspects." Each sample is digested using a mixture of two DNA restriction enzymes, which generates a distinct set of DNA fragments for each sample. The resulting DNA fragments are separated by agarose gel electrophoresis and visualized using Bio-Rad's revolutionary Fast Blast™ DNA stain.

Features and Benefits

  • Aligns with AP Biology Lab 6

With this kit, students are able to:

  • Study DNA restriction enzyme function
  • Use electrophoresis to separate DNA fragments
  • Use standard curves constructed from their data to make precise determinations of DNA fragment sizes

Applications and Uses

With the curriculum in this kit, students also have the opportunity to:

  • Read plasmid maps and predict the sizes of DNA fragments from restriction enzyme digests prior to performing the laboratory activity
  • Use restriction digest maps of lambda bacteriophage genomes (provided in the kit) to design novel plasmids
  • Learn how restriction enzymes function and how they are used in genetic engineering
  • Discuss scientific, ethical, and legal implications of forensics, DNA profiling, and genetic engineering

More Information

  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
DNA size standard (HindIII lambda DNA digest), 100 µl 1
DNA samples, lyophilized, 60 µg each 6
––Crime scene samples 1
––Suspect samples 5
EcoRl/Pstl restriction enzyme, lyophilized, 3,000 units 1
Sample loading buffer, 1 ml 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Sterile water, 2.5 ml 1
Agarose powder, 5 g 1
Fast Blast™ DNA Stain, 500x, 100 ml 1
Colored microcentrifuge tubes, 2.0 ml 80
Microcentrifuge tubes, 1.5 ml 30
Foam floats 8
Gel staining trays 4
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Forensic DNA Fingerprinting Kit

166-0007EDU
DNA evidence classroom study kit, includes DNA samples and standards, restriction enzymes, buffers, Fast Blast DNA stain, test tubes/holders, curriculum, and more, for 32 students; education use only, download free manual

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Number Description Options
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download
3050 Forensic DNA Fingerprinting Kit, Biotechnology Explorer, Quick Guide Please login to download this document
4106273 Forensic DNA Fingerprinting Kit, Lab Extension — Precut DNA Protocol, Biotechnology Explorer, Rev A Please login to download this document
1660077EDU Biotechnology Explorer™ Forensic DNA Fingerprinting Kit, Instruction Manual, Rev K Please login to download this document
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide, Rev A Click to download
5872 Biotechnology Explorer Refresh Kit Components Purchasing Guide, Rev A Click to download
5914 DNA Staining Using Fast Blast DNA Stain or SYBR Safe Stain and Digital Analysis of Gel Images Using Logger Pro Software, Rev A Click to download