Fish DNA Barcoding Kit

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PCR Amplification Kits - Fish DNA Barcoding Kit

Overview

DNA Barcode Generator

The DNA barcode generator allows you to create a barcode in color — each line of the barcode represents a particular base pair: green = adenine, blue = cytosine, black = guanine, red = thymine.

When assembled side by side, you will create a representative color barcode for the sequence of your sample!

Just follow the instructions to generate your barcode.

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Fish DNA Barcoding Kit Flowchart
Fish DNA Barcoding Kit Flowchart
Lab Preparation Checklist
Kit Contents (sufficient materials for 8 student workstations with 2–4 students per workstation)
Resuspension solution, 5 ml 1
Lysis solution, 5 ml 1
Neutralization solution, 5 ml 1
Wash buffer, 10 ml 1
Matrix, 5 ml 1
Spin filters 20
PCR tubes, 0.2 ml 50
Microcentrifuge tubes, 2 ml 250
PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml 1
Fish primer mix (40x), 50 µl 1
pCOI control, 25 µl 1
UView™ 6x loading dye, 200 µl 1
PCR molecular weight ruler, 200 µl 1
Sterile water, 2.5 ml 1
Curriculum (includes teacher guide, student manual, and graphic quick guide) 1
Required Accessories Not Included in Kit
Fish tissue samples 8–16
Digital dry bath/water bath 1
Model 16K centrifuge 1–2
Horizontal gel electrophoresis chambers 4–8
Power supplies 2–4
Thermal cycler 1
2–20 µl adjustable-volume micropipet 8
20–200 µl adjustable-volume micropipet 8
100–1,000 µl adjustable-volume micropipet 8
100–1,000 µl pipet tips 1 bag
2–20 µl aerosol barrier pipet tips 1 bag
20–200 µl aerosol barrier pipet tips 1 bag
UV transilluminator or imaging system 1
Ethanol 10 ml
Distilled water 5 ml
Razor blades or plastic knives 16
Electrophoresis buffer, 50x TAE, 100 ml 1
Agarose powder, 5 g 1
Gloves  
Markin pens 8
Recommended (Optional) Accessories
Precast agarose gels 8
Pipet controller 1
Vortexer 1
Digital camera for imaging gels 1
Key Kit Features
  • Inquiry-based hands-on laboratory
  • Lab kit can be completed in three 50-min laboratory sessions
  • The hands-on wet lab portion of the kit can be completed in three 50-min laboratory sessions
  • Real-world connection

The Fish DNA Barcoding Kit is an advanced PCR kit that allows students to determine the species of a fish sample based on its DNA sequence of the cytochrome c oxidase I gene.

Key Features and Benefits

With this kit, students are able to:

  • Perform DNA extraction, PCR amplification, and gel electrophoresis
  • Use sequencing and bioinformatics to determine fish species
  • Contribute to the global initiatives to barcode life

Background Information

Species are traditionally identified by taxonomists who need to have in-depth knowledge of certain species characteristics and environments in order to categorize them. The power of DNA research now allows us to add a level of identification based on the sequencing of a conserved gene. The subtle changes in sequences result in species-specific identification or genetic barcodes. This information is critical in helping to assess biodiversity and conservation efforts, as well as commercially, where food marketplaces can be regulated.

More Information

This kit provides sufficient materials for processing up to 16 fish and control samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation).

The DNA Barcoding Sequencing Module allows you to order sequencing services now and use them when you’re ready. Sample shipping to a validated sequencing facility is included with this module! PCR cleanup and sequencing are included for up to nine samples per sequencing module.

Kit Contents This kit provides sufficient materials for processing up to 16 fish samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation).
Cell resuspension solution, 5 ml 1
Cell lysis solution, 5 ml 1
Neutralization solution, 5 ml 1
Wash buffer, 10 ml 1
Matrix, 5 ml 1
Spin filters 20
PCR tubes, 0.2 ml 50
Microcentrifuge tubes, 2 ml 250
PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml 1
Fish primer mix (40x), 50 µl 1
pCOI control, 25 µl 1
UView™ 6x loading dye, 200 µl 1
PCR molecular weight ruler, 200 µl 1
Sterile water, 2.5 ml 1
Curriculum 1 (includes teacher guide, student manual, and graphic quick guide)
DNA Barcoding Sequencing Module  
PCR cleanup and sequencing for up to 9 samples 1 set for up to 9 samples
Fish DNA Barcoding Kit

166-5100EDU
Advanced PCR and bioinformatics kit, includes reagents for DNA extraction, PCR amplification, plastics, curriculum, for processing up to 16 fish and control samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation); education use only; sequencing module sold separately

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Number Description Options
6353 Fish DNA Barcoding Kit Planning Guide, Biotechnology Explorer, Rev A Click to download
6398 Biotechnology Explorer Fish DNA Barcoding Kit Bioinformatics Guide, Rev A Please login to download this document
10028899 Instruction Manual, Fish DNA Barcoding Kit, Biotechnology Explorer, Rev A Please login to download this document
6410 Instruction Manual, Biotechnology Explorer Fish DNA Barcoding Kit Quick Guide, Rev A Click to download
6602 UView™ 6x Loading Dye and Stain Guide, Rev A Click to download