Digital PCR Assays

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Digital PCR Assays

Overview

PrimePCR™ Lookup Tool

Use the PrimePCR lookup tool to find assays and panels for your genes of interest.

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PrimePCR™ Digital Assays – Performance Data

PrimePCR digital assays are predesigned, lab-validated assays. The following data illustrate the performance of the mutation detection and copy number variation assays:

Mutation Detection Assays
Mutation detection assays on the ddPCR™ platform enable detection of 0.001% mutant fractions because partitioning increases sensitivity by isolating the target signal from competing background.

High sensitivity of PrimePCR™ ddPCR™ mutation detection assays provides precise quantification of JAK2 V617F mutation in the presence of wild-type DNA.

Mutation Detection Assays

A, 2-D fluorescence amplitude plot shows duplicate wells of a mixed mutant:wild-type sample across multiple percentage mutant blends. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for the wild-type DNA only, the blue cluster represents the droplets that are positive for the mutant DNA only, and the orange cluster represents the droplets that are positive for both wild-type and mutant targets;. B, 2-D fluorescence amplitude plot shows duplicate wells of a wild-type only sample; C, fractional abundance plot shows the percentage frequency (orange markers) of the mutant DNA in a wild-type DNA background. All error bars generated by QuantaSoft™ software represent the 95% confidence interval.



Copy Number Variation Assays
PrimePCR ddPCR copy number assays provide superior resolution and precision. The massive partitioning of a copy number variation ddPCR reaction across up to 20,000 droplets enables the discrimination required to determine small fold changes.

Copy Number Variation Assays

A, 2-D fluorescence amplitude plot shows four replicate wells of a copy number sample duplexed with ERBB2 and RPP30 assays. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for the RPP30 reference only, the blue cluster represents the droplets that are positive for ERBB2 only, and the orange cluster represents the droplets that are positive for both ERBB2 and RPP30 targets;. B, copy number plot shows multiple samples at copy number two with precise replicate and merged well values; C, copy number plot shows multiple oncogene copy number determinations with the same NCI-H358 lung cancer sample. All error bars generated by QuantaSoft software represent the 95% confidence interval.

PrimePCR™ ddPCR™ Assays Brochure

PrimePCR™ Assays for
Droplet Digital™ PCR

Expertly designed and validated assays

PrimePCR™ ddPCR™ Assays are validated assays that enable precise quantification without a standard curve.

  • Generate consistent and reproducible results
  • Utilize universal cycling conditions and primer/probe strategy
  • Guarantee performance

Download PDF


 
QX200™ Droplet Digital™ PCR System Brochure

Droplet Digital PCR (ddPCR™) is Bio-Rad's unique digital PCR technology.

  • Unrivaled precision provides absolute quantification of target DNA or RNA molecules without the use of standard curves
  • Addresses the lack of scalable and practical technologies for digital PCR implementation

The QX200 ddPCR system makes this powerful technology accessible to you for unveiling previously unattainable
research levels.

Download PDF


 

Digital PCR Assay Kits

Bio-Rad also offers ddPCR Assay Kits that consist of probe-based assays, positive controls and ddPCR Supermix for Probes (No dUTP) for research use.

Our digital PCR assays are hydrolysis probe–based assays that were designed by experts in the digital PCR field. Every digital PCR assay has been experimentally validated to ensure optimal performance for its target application:

  • Copy number variation (CNV) analysis — determine small changes in copy number without the use of a standard curve
  • Mutation detection — detect one mutant in a background of at least 2,000 wild-type molecules

These predesigned assays for digtal PCR come with a FAM or HEX Fluorophore.

Digital PCR assays are based on Droplet Digital™ PCR (ddPCR™) technology and feature unrivaled accuracy, precision, and sensitivity in providing an absolute measure of target DNA molecules.

When paired with the QX200™ Droplet Digital™ PCR System and our optimized ddPCR™ Supermix for Probes (No dUTP), PrimePCR™ ddPCR™ Assays ultimately accelerate discovery and enable new research strategies for inherited disorders, cancer, and neurological diseases.

Droplet Digital PCR applications guide Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:
  • Copy number variation analysis
  • Rare mutation and sequence detection
  • Gene expression
  • Next-generation sequencing library analysis

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PrimePCR  ddPCR  Mutation Assay BRAF WT for p.V600E and BRAF p. V600E  Human
PrimePCR™ ddPCR™ Mutation Assay Kit: BRAF WT for p.V600E, and BRAF p.V600E, Human

1863100
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: BRAF WT for p.V600K, and BRAF p.V600K, Human

1863101
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: BRAF WT for p.V600R, and BRAF p.V600R, Human

1863102
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: EGFR WT for p.T790M, and EGFR p.T790M, Human

1863103
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: EGFR WT for p.L858R, and EGFR p.L858R, Human

1863104
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: EGFR WT for p.E746_A750del, and EGFR p.E746_A750del, Human

1863105
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: EGFR WT for p.L861Q, and EGFR p.L861Q, Human

1863106
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: EGFR WT for p.G719S, and EGFR p.G719S, Human

1863107
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: HRAS WT for p.G12V, and HRAS p.G12V, Human

1863108
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KIT WT for p.D816V, and KIT p.D816V, Human

1863109
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.G12A, and KRAS p.G12A, Human

1863110
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.G12C, and KRAS p.G12C, Human

1863111
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.G12D, and KRAS p.G12D, Human

1863112
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.G12R, and KRAS p.G12R, Human

1863113
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.G12S, and KRAS p.G12S, Human

1863114
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.G12V, and KRAS p.G12V, Human

1863115
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.G13D, and KRAS p.G13D, Human

1863116
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: KRAS WT for p.A146T, and KRAS p.A146T, Human

1863117
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: AKT1 WT for p.E17K, and AKT1 p.E17K, Human

1863118
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: FGFR2 WT for p.S252W, and FGFR2 p.S252W, Human

1863119
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: FLT3 WT for p.D835Y, and FLT3 p.D835Y, Human

1863120
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: GNA11 WT for p.Q209L, and GNA11 for p.Q209L, Human

1863121
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: GNAQ WT for p.Q209L, and GNAQ for p.Q209L, Human

1863122
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: IDH1 WT for p.R132C, and IDH1 p.R132C, Human

1863123
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: IDH1 WT for p.R132H, and IDH1 p.R132H, Human

1863124
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: IDH2 WT for p.R140Q, and IDH2 p.R140Q, Human

1863125
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: JAK2 WT for p.V617F, and JAK2 p.V617F, Human

1863126
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: NRAS WT for p.Q61H, and NRAS p.Q61H, Human

1863127
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: NRAS WT for p.Q61L, and NRAS p.Q61L, Human

1863128
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: NRAS WT for p.Q61K, and NRAS p.Q61K, Human

1863129
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: NRAS WT for p.Q61R, and NRAS p.Q61R, Human

1863130
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: PIK3CA WT for p.E542K, and PIK3CA p.E542K, Human

1863131
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: PIK3CA WT for p.E545K, and PIK3CA p.E545K, Human

1863132
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay Kit: PIK3CA WT for p.H1047R, and PIK3CA p.H1047R, Human

1863133
ddPCR™ probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ ddPCR™ Mutation Detection Assay: IDH2 WT for p.R172K, and IDH2 WT for p.R172K, Human

1863134
ddPCR probe assay designed for mutation detection. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Copy Number Reference Assay Kit: EIF2C1, Human

1863300
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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RUB24,865.00
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PrimePCR™ ddPCR™ Copy Number Reference Assay Kit: AP3B1, Human

1863301
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Copy Number Assay Kit: EGFR, Human

1863302
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Copy Number Assay Kit: KRAS, Human

1863303
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Copy Number Assay Kit: BRCA1, Human

1863304
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Copy Number Assay Kit: BRCA2, Human

1863305
ddPCR™ probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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PrimePCR™ ddPCR™ Copy Number Assay Kit: ERBB2 Kit, Human

1863306
ddPCR probe assay designed for copy number variation analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe

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Number Description Options
6512 PrimePCR Assays for Droplet Digital PCR Brochure, Rev A Click to download [ Add to Cart (Free) ]
10041702 PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A Click to download
10033173 PrimePCR™ ddPCR™ Copy Number Variation (CNV) Assays Product Insert, Rev B Click to download
10033487 PrimePCR™ ddPCR™ Mutation Detection Assays Product Insert, Rev B Click to download
10047489 PrimePCR™ ddPCR™ Mutation Detection Assays (in silico designed) Product Insert, Rev A Click to download
6630 ddPCR™ SMN1 Copy Number Determination Kit Flier, Rev B Click to download [ Add to Cart (Free) ]
6631 ddPCR™ SMN2 Copy Number Determination Kit Flier, Rev B Click to download [ Add to Cart (Free) ]
6632 ddPCR™ KRAS Screening Multiplex Kit Flier, Rev A Click to download [ Add to Cart (Free) ]
10048179 ddPCR™ KRAS Screening Multiplex Kit Product Insert, Rev A Click to download
6311 QX200™ Droplet Digital™ PCR System Brochure, Rev C Click to download [ Add to Cart (Free) ]
6407 Droplet Digital PCR Applications Guide, Rev A Click to download
6338 Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A Click to download [ Add to Cart (Free) ]
10026235 ddPCR™ Supermix for Probes Product Insert, Rev C Click to download
10026868 ddPCR™ Supermix for Probes (No dUTP) Product Insert, Rev D Click to download
10040717 QX200 Droplet Generation Oil for EvaGreen Product Insert, Rev B Click to download
10040718 Droplet Generation Oil for Probes Product Insert, Rev B Click to download
6450 Droplet Digital™ PCR Publications List, Rev K Click to download
6495 Bio-Rad’s ddPCR™ Library Quantification Kit for Ion Torrent Enables Accurate Library Quantification and Excellent Balancing of Pooled Libraries, Rev A Click to download [ Add to Cart (Free) ]
6679 Droplet Digital™ PCR: Multiplex Screening of KRAS Mutations in Cell-Free DNA Colorectal Cancer Samples Application Note, Rev A Click to download
6628 Rare Mutation Detection Best Practices Guidelines Click to download
10048730 PrimePCR™ ddPCR™ Gene Expression Probe Assays, RevA Click to download