The Crime Scene Investigator PCR Basics™ Real-Time PCR Starter Kit is a tool for teaching students the principles of PCR and its use in forensic DNA analysis. This kit is a good starting point for teaching real-time PCR to novices to familiarize them with this valuable technique. Additionally, DNA fingerprints can still be investigated using gel electrophoresis and melt-curve analysis, showing how real-time and conventional PCR can be complementary techniques.
Features and Benefits
With this kit, students are able to:
- Quantitate DNA
- Discover key differences between conventional and real-time PCR analysis
- Analyze and evaluate real-time PCR results and perform melt-curve analysis
- Determine the accuracy and reliability of pipetting techniques
- Learn the molecular basis of DNA amplification reactions using real-time PCR detection systems
Applications and Uses
This kit can be extended to demonstrate real-time PCR in two different labs. Each may be performed separately or run in sequence for greater depth of understanding and increased hands-on experience.
Laboratory scenario 1 preserves the original intent of the Crime Scene Investigator PCR Basics Kit, allowing students to observe real-time PCR as the reactions progress and to amplify and compare suspect versus crime scene DNA samples using agarose gel electrophoresis.
Laboratory scenario 2 is more advanced and modifies the Crime Scene Investigator PCR Basics Kit protocol to include a DNA dilution series and an optional very fast PCR protocol. This lab allows for a rapid demonstration of the quantitative nature of real-time PCR and melt-curve analysis, and in doing so uses an entirely different experimental design than the core Crime Scene Investigator PCR Basics Kit.
- Three-session laboratory activity, 45 min per session
- Provides sufficient materials for eight student workstations, up to four students per workstation