Comparative Proteomics Kit I: Protein Profiler Module

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Comparative Proteomics Kit I: Protein Profiler Module

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Laemmli sample buffer, 30 ml 1
Precision Plus Protein Kaleidoscope prestained standards, 50 µl 1
10x Tris-glycine-SDS electrophoresis buffer, 1 L 1
Bio-Safe Coomassie stain for proteins, 100 ml 2
Actin and myosin standard, 500 µg lyophilized 1
Dithiothreitol (DTT), 0.3 g 1
Prot/Elect pipet tips for gel loading 1 rack
1.5 ml fliptop micro test tube 60
1.5 ml screwcap micro test tube 50
Disposable plastic transfer pipets 30
Gel-staining trays 4
Foam micro test tube holders 8
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:  
Fish samples 5–8 types
Adjustable micropipets, 2–20 µl 8
Power supplies 2–4
Water bath 1
If using polyacrylamide gel electrophoresis:
Required Accessories:
 
Vertical gel electrophoresis chambers 2–4
Ready Gel precast polyacrylamide gels, 15%, 10-well each 8
If using agarose gel electrophoresis:
Required Accessories
 
Horizontal gel electrophoresis chambers 4–8
Low-melt agarose, 25 g  
Pipet tips, 2–20 µl, TBR-35  
Acetic acid, 100 ml  
Reagent alcohol/ethanol, 400 ml  
Recommended (Optional) Accessories:  
Sample loading guides  
GelAir drying system  
Protein Profiler Flowchart
Protein Profiler Flowchart
Key Kit Features
  • Explore evolution
  • Study protein structure/function
  • Apply protein electrophoresis
  • Construct cladograms
  • Complete in three 45 minute lab sessions
Protein Profiler Curriculum Fit
Protein Profiler Curriculum Fit
Theory of Evolution

This kit guides students through the thought processes involved in a laboratory-based scientific investigation. Students are asked: Can molecular evidence support the theory of evolution? Why or why not? What explanations can you suggest?

Changes in proteins can reflect changes in the gene pool. Actin and myosin are the major muscle proteins essential for locomotion and survival in all animals. Muscle consists mainly of actin and myosin, but numerous other proteins also comprise muscle tissue. The structures and functions of actin and myosin have remained relatively stable or "conserved" in all animals over evolutionary time. However, other muscle proteins exhibit considerable variation even among closely related species. Detectable variations between organisms' protein profiles reflect physiological adaptations to different environments, but they originate as random DNA mutations. Such mutation events, if favorable, persist through the natural selection process and contribute to the evolution of species — with new specialized functions.

Mutation > Variation > Specialization > Speciation = Evolution

This is an open-ended inquiry-based kit. Students make predictions about their results in prelab activities using internet databases and published phylogenetic information. They generate novel results and apply their findings directly to the problem of solving evolutionary relationships by constructing cladograms (phylogenetic trees). From their gel data, they build up a tree and assign each organism a branch. Students can decide whether their results support their predictions.

Protein Profiler Gel

Protein Profiler Gel

PowerPoint Presentation for Workshops and for Teaching

Comparative Proteomics Kit I: Protein Profiler Module

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Comparative Proteomics Kit I: Protein Profiler Module and includes instructions for using the kit.

Comparative Proteomics Kit I: Protein Profiler Module PowerPoint presentation

Comparative Proteomics Kit I: Protein Profiler Module

The Comparative Proteomics I Kit: Protein Profiler Module guides students through the thought processes involved in a laboratory-based scientific investigation. Students make predictions about their results in prelab activities using Internet databases and published phylogenetic information. They then employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function, generating protein profiles from the muscles of both distantly and closely related species of fish. From their results, they compare the different species' profiles, construct cladograms (phylogenetic trees), and assign each organism a branch. Students can decide whether their results support their predictions.

Features and Benefits

With this kit, students are able to:

  • Explore evolution
  • Study protein structure and function
  • Use polyacrylamide gel electrophoresis (PAGE) to separate proteins by size
  • Construct cladograms using data from their gel analysis

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Laemmli sample buffer, 30 ml 1
Precision Plus Protein™ Kaleidoscope™ prestained standards, 50 µl 1
10x Tris-glycine-SDS electrophoresis buffer, 1 L 1
Bio-Safe™ Coomassie stain for proteins, 100 ml 2
Actin and myosin standard, 500 µg lyophilized 1
Dithiothreitol (DTT), 0.3 g 1
Prot/Elect™ pipet tips for gel loading, rack 1
1.5 ml fliptop micro test tube 60
1.5 ml screwcap micro test tube 50
Disposable plastic transfer pipets 30
Gel-staining trays 4
Foam micro test tube holders 8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Comparative Proteomics Kit I: Protein Profiler Module

166-2700EDU
Protein profiling classroom study kit, includes buffers, standards, stains, DTT, test tubes/holders, disposable pipets/tips, trays, curriculum, and more, for 32 students; education use only

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Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module

166-2850EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (#166-2700EDU) and Western Blot Module (#166-2800EDU), for 32 students; education use only

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Rapid Blotting and V3 Western Workflow™ Starter Kit

166-2875EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (#166-2700EDU), Western Blot Module (#166-2800EDU), Trans-Blot® Turbo™ mini nitrocellulose transfer packs (10), 4–20% Mini-PROTEAN® TGX Stain-Free™ gels (10), application note, for 32 students; education use only

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Number Description Options
M1662876EDU Biotechnology Explorer Rapid Blotting + V3 Western Workflow (Stain-Free Rapid Blotting) Application Note, Rev A Click to download
5464 Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module Quick Guide, Biotechnology Explorer, Rev A Click to download
10004530 Instruction Manual, Comparative Proteomics Kit I: Protein Profiler Module, Biotechnology Explorer, Rev F Please login to download this document [ Add to Cart (Free) ]
5478 Instruction Manual, Comparative Proteomics Kit II: Western Blot Module Quick Guide, Biotechnology Explorer, Rev A Click to download