Chromatography Standards

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Overview

Ion Exchange Chromatography Standards
  Contents MW pI For Use With
Anion exchange standard Equine myoglobin 17,000 6.9 UNO Q; Econo-Pac UNOsphere Q, High
  Conalbumin 77,000 4.9  
  Chicken ovalbumin 45,000 4.6  
  Soybean trypsin inhibitor 15,500 4.5  
Cation exchange standard Equine myoglobin 17,000 6.9 17,000 6.9 UNO S, Bio-Scale S, Econo-Pac S, CM, and UNOsphere S cartridges; Macro-Prep High S and CM media; UNOsphere S support
  Ribonuclease A 13,500 8.7  
  Cytochrome c 12,000 10.7  
Organic Acid Standard
Contents MW For Use With
Sodium oxalate 134 Aminex HPX-87H column; organic acid analysis kit
Sodium citrate 294  
Sodium malate 196  
Sodium succinate 270  
Sodium formate 69  
Sodium acetate 82  
Carbohydrate Standard
Contents MW For Use With
Melezitose 504 Aminex HPX-87C column; carbohydrate analysis kit
Maltose 360  
Glucose 180  
Mannose 180  
Fructose 180  
Ribitol 152  
Gel Filtration Chromatography Standard
Contents MW pI For Use With
Thyroglobulin 670,000 4.5 Bio-Sil and Bio-Silect SEC columns; Bio-Gel P media
Bovine gamma-globulin 158,000 5.1  
Chicken ovalbumin 44,000 4.6  
Equine myoglobin 17,000 6.9  
Vitamin B12 1,350 4.5  

Standards are available for ion exchange, size exclusion (gel filtration), and hydrophobic interaction chromatography, as well as for analysis of organic acids and carbohydrates.

Ion Exchange Chromatography Standards

Bio-Rad offers two standards for ion exchange chromatography, both suitable for use with bulk resin, cartridges, or columns. Each standard is supplied as a set of six vials of lyophilized protein mixture for qualitative analysis only.

Gel Filtration Chromatography Standard

Bio-Rad's gel filtration standard is a calibration standard for size exclusion columns used in protein purification. The mixture includes vitamin B12 and myoglobin, which are visible when eluting from glass or clear plastic columns and can be used to ensure that the column is properly packed and the sample is eluting evenly. The standard can be used with most size exclusion HPLC columns. The standard is supplied as a set of six vials of lyophilized protein mixture.

Organic Acid Standard

Bio-Rad's organic acid standard is supplied as a set of six vials of lyophilized mixture for qualitative analysis only.

Carbohydrate Standard

Bio-Rad's carbohydrate standard is supplied as a set of six vials of lyophilized mixture for qualitative analysis only. The standards can be used for column testing or semiquantitative determination.

Protein Standard for Anion Exchange Chromatography

125-0561
Pkg of 6 vials, lyophilized mixture of equine myoglobin, conalbumin, chicken ovalbumin, soybean trypsin inhibitor, for ion exchange chromatography

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Protein Standard for Cation Exchange Chromatography

125-0562
Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography

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Organic Acid Analysis Standard

125-0586
Pkg of 6 vials, lyophilized mixture of oxalate, citrate, malate, succinate, formate, and acetate sodium salts; for use in organic acid analysis

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Carbohydrate Analysis Standard

125-0585
Pkg of 6 vials, lyophilized mixture of melezitose, maltose, glucose, mannose, fructose, and adonitol (ribitol); for use in carbohydrate analysis

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Gel Filtration Standard

151-1901
Pkg of 6 vials, lyophilized mix of thyroglobulin, bovine γ-globulin, chicken ovalbumin, equine myoglobin, and vit B12, MW 1,350–670,000, pI 4.5–6.9

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Number Description Options
LIT585 Instruction Manual, Organic Acid Analysis Standard Click to download
LIT584 Instruction Manual, Carbohydrate Analysis Standard Click to download
LIT46 Instruction Manual, Protein Standard for Ion Exchange HPLC Click to download
LIT102 Instruction Manual, Gel Filtration Standard, Rev E Click to download
LIT162 Instruction Manual, Protein Standard for Hydrophobic Chromatography, Rev B Click to download