ChemiDoc™ MP System

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ChemiDoc MP System – Gel Imaging Systems

Overview

ChemiDoc™ MP Instrument Tour

ChemiDoc™ MP Imager Testimonial


JiJoon Song Assistant Professor
,
Department of Biological Sciences, KAIST
Daejeon, South Korea

Imaging System Selection Guide
  ChemiDoc™ MP GelDoc™ EZ GS-900 Gel Doc XR+ and ChemicDoc XRS+ PharosFX™, PharosFX Plus, Personal Molecular Imager
ChemiDoc™
MP
GelDoc™
EZ
GS-900™ Gel Doc
XR+
ChemiDoc
XRS+
PharosFX™ PharosFX Plus Personal Molecular Imager™
Nucleic Acid Detection
Ethidium bromide 5 4 - 4 4 5 5 -
SYBR® Green I stain 5 4 - 4 4 5 5 -
SYBR® Safe stain 5 4 - 4 4 5 5 -
Coumarin stain 5 4 5 - - - - -
Fast Blast™ DNA stain 4 4 5 4 4 - - -
Protein Staining, 1-D Gels
Stain-free gels 5 5 - 5 5 - - -
Coomassie Blue stain 4 4 5 4 4 2 2 -
Silver stain 4 4 5 4 4 2 2 -
SYPRO Ruby protein blot stain 4 4 - 4 4 5 5 -
Flamingo™ fluorescent gel stain 4 3 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 4 - 5 5 - - -
Protein Staining, 2-D Gels
Coomassie Blue stain 4 3 5 3 3 2 2 -
Silver stain 4 3 5 3 3 2 2 -
SYPRO Ruby protein blot stain 4 3 - 3 3 5 5 -
Flamingo™ fluorescent gel stain 4 2 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 3 3 3 3 3 3 3
Pro-Q stain 4 2 - 2 3 5 5 -
Cy2, Cy3, Cy5 label 4 - - - - 5 5 -
Blot Detection
Stain-free blots 4 5 - 4 5 - - -
Colorimetric blots 3 3 5 3 3 2 2 2
Coomassie Blue stain 4 - 5 4 4 2 2 -
Silver stain 5 - 5 4 4 2 2 -
SYPRO Ruby protein blot stain* 5 - - - - 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Chemifluorescence* 5 - - 1 1 4 4 -
Qdot* 5 2 - 2 2 5 5 -
Multiplex fluorescence 5 - - - - - - -
Micro- and Macroarray** Detection
Radiolabel - - - - - - 5 5
Fluorescence 5 - - 2 2 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Colony Counting
Colorimetric detection 5 - - 4 4 3 3 -
Fluorescent detection 5 - - 4 4 5 5 -
Isotopic
Radiolabel - - - - - - 5 5
X-ray film 4 - 5 4 4 3 3 -

- Not recommended; 1–5, recommendation level (5 = highest).
*    Optimal with low fluorescence PVDF membrane.
**   With spot features ≥400 µm.

Precision Plus Protein™ WesternC™ Standards

Gel (left), chemiluminescent detection (middle), fluorescent detection (right).

The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications.

Its features are based on CCD high-resolution, high-sensitivity detection technology, and modular options to accommodate a wide range of samples and support multiple detection methods. The system is controlled by Image Lab™ software to optimize performance for fast, integrated, and automated image capture and analysis of various samples.

Features and Benefits

  • Multiple imaging capabilities — the ChemiDoc MP imager can accommodate a variety of sample types and detection methods including multiplex fluorescent western blotting. It is the perfect imager to accompany your protein and DNA electrophoresis runs as well as your western blotting experiments. It delivers quantitative, reproducible results for fluorescence, chemiluminescence, and colorimetric detection
  • Stain-free technology — UV-induced fluorescence labeling of proteins in the stain-free gels allows a 2 hr Coomassie gel-staining protocol to be condensed into a 5 min stain-and-image step. Stain-free gels are western blot compatible — using the V3 Western Workflow™, check your electrophoresis results and blot transfer quality prior to western blotting
  • High-sensitivity blot detection — the ChemiDoc MP imaging system offers advanced detection technology that determines optimal exposure, even for faint or intense samples. Superior sensitivity is achieved for chemiluminescence and multiplex fluorescence detection and for colorimetric gel and blot documentation
  • Superior Image Quality — exceptional dynamic range enables visualization of faint and intense bands on same blot or gel. Images are always in focus at any zoom level to ensure publication-ready images in seconds
  • Ease of Use — precalibrated system provides the precise focus for any zoom setting or sample height; automated hands-free operation ensures consistent, reproducible, and high-throughput performance
Applications  
Chemiluminescence Yes
Fluorescence Yes
Colorimetry/densitometry Yes
Gel documentation Yes
Hardware Specifications  
Maximum sample size 28 x 36 cm
Maximum image area 26 x 35 cm
Maximum image area (For standard UV-excited gels) 25 x 26 cm
Excitation source Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen, XcitaBlue™ conversion screen, and epi-red, epi-green, and epi-blue LED modules available
Illumination control 8 modes available. Trans-UV, epi-white, and no illumination for chemiluminescence are standard; epi-red, epi-green, epi-blue, trans-white, and XcitaBlue conversion screens are optional
Detector Supercooled CCD
Image resolution 4 megapixels
Pixel size (H x V) 6.45 x 6.45 µm
Cooling system Peltier
Camera cooling temperature -30°C absolute and regulated
Filter holder 6 positions (5 for filters, 1 without filter for chemiluminescence)
Emission filters 1 included (standard), 4 optional
Dynamic range >4.0 orders of magnitude
Pixel density (gray levels) 65,535
Dynamic flat fielding Application specific; for all applications
Instrument size (L x W x H) 36 x 60 x 96 cm
Instrument weight 32 kg
Operating Ranges  
Operating voltage 110/115/230 VAC nominal
Operating temperature 10–28°C (21°C recommended)
Operating humidity, noncondensing <70%
Automation Capabilities  
Workflow automated selection Application driven; user selected or recalled by a protocol
Workflow automated execution Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
Workflow reproducibility 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
Autofocus (patent pending) Precalibrated focus for any zoom setting or sample height
Image flat fielding** Dynamic; precalibrated and optimizor every application
Autoexposure 2 user-defined modes (intense or faint bands)
**U. S. patent 5,951,838  
ChemiDoc MP System
ChemiDoc™ MP System

170-8280
Gel imaging system, includes darkroom, UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab™ software compatible with Windows or Mac OS

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ChemiDoc™ MP Red LED Module Kit

170-8283
Pkg of 2 epi-red LED modules and 1 red emission filter, for use with applications requiring red fluorophore detection

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ChemiDoc™ MP Green LED Module Kit

170-8284
Pkg of 2 epi-green LED modules and 1 green emission filter, for use with applications requiring green fluorophore detection

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ChemiDoc™ MP Blue LED Module Kit

170-8285
Pkg of 2 epi-blue LED modules and 1 blue emission filter, for use with applications requiring blue fluorophore detection

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Number Description Options
6427 Bio-Rad V3 Western Workflow, Rev A Click to download [ Add to Cart (Free) ]
10040758 Instruction Manual, Image Lab Software: Imaging Stain-Free Gels and Blots, Rev B Click to download
6434 Western Blot Normalization Using Image Lab™ Software Quick Start Guide Click to download
10016116 Gel Alignment Template Kit Click to download
10022469 Instruction Manual, Image Lab Software 5.1 for the ChemiDoc MP System Click to download
6126 Imaging Systems, Computer Specifications, Rev D Click to download
6133 ChemiDoc MP Brochure, Rev C Click to download [ Add to Cart (Free) ]
6253 V3 Western Workflow Brochure with ChemiDoc MP Imager, Rev B Click to download [ Add to Cart (Free) ]
5576 Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C Click to download [ Add to Cart (Free) ]
6166 Multiplex Fluorescent Blot Detection: A Troubleshooting Guide, Rev A Click to download [ Add to Cart (Free) ]
2589 Not All Bits Are Created Equal Click to download
4000177 Instruction Manual, IPD Multi-Color Imaging Target, Rev A Click to download
3097 Imaging and Analysis: Tools for Acquisition and Analysis of Protein Expression Data Brochure, Rev B Click to download [ Add to Cart (Free) ]
RP0051 Stain-Free Approach for Western Blotting Click to download
5888 Bio-Rad Imaging Systems Family Brochure, Rev E Click to download [ Add to Cart (Free) ]
6147 Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B Click to download [ Add to Cart (Free) ]
RP0058 A Defined Methodology for Reliable Quantification of Western Blot Data Click to download