CFT™ Ceramic Fluoroapatite Media

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CFT ceramic fluoroapatite, Ca10(PO4)6F2, is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5.6 with minimal effect on the solubility or lifetime of the support.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Additional features of CFT include:

  • High-density particles for fast, simple column packing
  • Sintering at 650°C (Type II) for heavy-duty, durable supports
  • Rigid particles for fast cleaning and equilibration
  • Inorganic calcium phosphate for distinct selectivities

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Functional groups Ca2+, PO4 3-, F-
Nominal mean particle size 40 ± 4 µm
Nominal pore diameter range 600–800 A
Packing density (g/ml packed bed) .86 g/ml
Dynamic binding capacity 14–21.5 mg lysozyme/g
Typical IgG binding capacity 33 mg/ml
Maximum operating pressure 55 bar (800 psi)
Operating pH range 5–14
Regeneration Normal conditions: 400 mM sodium phosphate, pH 7.4; Difficult conditions: 400–1,000 mM sodium phosphate, pH 11–12
Sanitation 1–2 M NaOH or KOH
Storage pH range 11–14
CFT Ceramic Fluoroapatite #158-5200
CFT Ceramic Fluoroapatite

158-5200
10 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

157-5000
100 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

157-5100
1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

157-5500
5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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Number Description Options
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
3111 CFT Ceramic Fluoroapatite Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5853 Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT Ceramic Hydroxyapatite and CFT Ceramic Fluoroapatite Chromatographic Media, Rev A Click to download [ Add to Cart (Free) ]
5913 Separation of Fab and Fc Fragments from Monoclonal Antibody Papain Digest on Ceramic Hydroxyapatite and Ceramic Fluoroapatite, Rev A Click to download