Cell Proliferation Assays

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Overview

Properties of Cell Proliferation Dyes
Available Dyes Fluorescent Proteins (FP) Excitation Maximum, nm Emission Maximum, nm Optimal Excitation Laser, nm 488/561
S3™ Filter Set, nm
Catalog Number
CytoTrack™ Blue 403/454 GFP
eGFP
YFP
403 454 405 FP 135-1202
CytoTrack
CytoTrack Green 511/525 DsRed
tdTomato
511 525 488 FP 561
FL2 (586/25)
135-1203
CytoTrack 488
FL1 (525/30)
mCherry 511 525 488 FP 561
FL3 (615/25)
CytoTrack 488
FL1 (525/30)
CytoTrack Yellow 542/556 GFP
eGFP
YFP
542 556 532 FP 135-1204
CytoTrack
CytoTrack Red 628/643 GFP
eGFP
YFP
628 643 633 or 640 FP 135-1205
CytoTrack
CFDA-SE   492 517 488 FL1 (525/30) 135-1201


CytoTrack™ Cell Proliferation Assays

CytoTrack cell proliferation assays are designed to efficiently stain live cells for excellent resolution of each cell division generation.

Using a proprietary chemistry, the dye reacts with primary amines and is retained in the cell with minimal efflux, allowing up to 10 cell divisions to be resolved.

As cell division occurs, the dye is successively halved and the different generations can be determined based on the decrease in fluorescence intensities.

CytoTrack CFDASE Graph
Cell division monitoring using CytoTrack Green 511/525 (A) and compared to CFDA-SE (B). CytoTrack Green shows a reduced efflux between generation 0 and 1.

Key Benefits of CytoTrack Cell Proliferation Dyes

  • Fix and permeabilize cells stained with CytoTrack dyes for analysis of intracellular targets— compatible with standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
  • Combine CytoTrack assays with fluorescent proteins or antibody-labeled fluorophores for multicolor cell analysis
  • Save time by using the cell proliferation assay directly with your cell culture media
  • Detect up to 10 cell divisions

Related Products

For convenient CFSE staining, we offer an additional cell proliferation assay featuring CFDA-SE dye (135-1201) to visualize up to 8 cell divisions.

Additional Products for Flow Cytometry

More Information

Learn more about the features of the S3™ cell sorter and its associated consumables and accessories.

CytoTrack Blue 403/454
CytoTrack™ Blue 403/454

135-1202
Pkg of 1, kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays

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CytoTrack Green 511/525
CytoTrack™ Green 511/525

135-1203
Pkg of 1, kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays

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CytoTrack Yellow 542/556
CytoTrack™ Yellow 542/556

135-1204
Pkg of 1, kit includes 4 x 50 assay vials and 250 μl of DMSO, for running 200 assays

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CytoTrack Red 628/643
CytoTrack™ Red 628/643

135-1205
Pkg of 1, kit includes 4 x 50 assay vials and 250 µl of DMSO, for running 200 assays

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CFDA-SE
CFDA-SE

135-1201
Pkg of 1, 5 x 100 µg vials

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Number Description Options
6524 CytoTrack Cell Proliferation Assay Kits Flier, Rev A Click to download