Bio-Plex Pro™ Rat Cytokine, Chemokine, and Growth Factor Assays

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Bio-Plex Pro Rat Cytokine, Chemokine, and Growth Factor Assays

Overview

Available Bio-Plex Pro™ Rat Cytokine Assays


23-Plex
(12005641)
12-Plex
Th1/Th2 Panel
(171K1002M)
TGF-β 3-Plex
(171W4001M)
Singleplex Sets
Bead Regions
G-CSF
 
 
54
GM-CSF
  37
GRO/KC
    57
IFN-γ
  34
IL-1α
  21
IL-1β
  28
IL-2
  22
IL-4
  33
IL-5
  52
IL-6
  56
IL-7
    38
IL-10
  19
IL-12p70
  78
IL-13
  15
IL-17A
    72
IL-18*
      20
M-CSF
    26
MCP-1
    51
MIP-1α
    77
MIP-3α
    36
RANTES
    55
TNF-α
  43
VEGF
    47
IL-12p40**       76
MIP-2***       27
TGF-β1    
13
TGF-β2    
72
TGF-β3    
66


* IL-18 is not available as a singleplex assay but only as part of an assay panel.

** IL-12p40 cannot be multiplexed with IL-12p70.

*** MIP-2 cannot be multiplexed with GRO/KC.

Bio-Plex Pro™ Rat Cytokine Assay Specifications

Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in a serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for <20% intra-assay CV, <30% inter-assay CV, and recovery range of 80–120%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

 

Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets
LLOQ
ULOQ
LOD
Intra-assay %CV Inter-assay %CV
Singleplex Assays
IL-12p40 2 2,015 0.4 3 4
MIP-2 0.3 1,085 0.2 4 5
 
Multiplex Assays
EPO 32 32,258 8 4 1
G-CSF 1 2,911 0.2 2 1
GM-CSF 6 31,519 0.6 4 1
GRO-KC 2 1,521 0.6 4 2
IFN-gamma 4 23,955 1 5 3
IL-1alpha 2 24,649 1 3 3
IL-1beta 6 31,666 2 4 2
IL-2 8 7,981 3 4 3
IL-4 1 16,209 1 6 3
IL-5 6 2,007 6 4 0.4
IL-6 13 30,766 10 4 3
IL-7 2 32,472 0.4 4 3
IL-10 4 34,385 5 4 6
IL-12p70 4 15,481 0.7 4 3
IL-13 2 15,751 0.9 4 2
IL-17 1 3,079 0.1 3 2
IL-18 4 31,652 4 4 5
M-CSF 2 23,264 0.4 2 3
MIP-1alpha 25 4,325 12 4 7
MIP-3alpha 1 3,032 0.7 4 2
RANTES 8 7,950 3 5 2
TNF-alpha 16 8,207 3 5 7
VEGF 0 3,963 0.3 3 5

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is two-fold of the standard deviation above the background signal

TGF-β Assay Specifications Overlay
Incubation time
3-Plex Panel
(171-W4001)
Sample volume
12.5 µl
Accuracy (recovery range)
80–100%
Precision
   Intra-assay %CV
   Inter-assay %CV

   ≤10%
   ≤15%


Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for 10% intra-assay CV, 15% inter-assay CV, and recovery range of 80–100%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

TGF-β Assay Specifications
 
Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets LLOQ ULOQ LOD Intra-assay %CV Inter-assay %CV
Premixed 3-Plex Assay
TGF-β1 1.69  27,616 3.9
4.5
4.9
TGF-β2
14.7 
30,080 1.9
6.3 
9.1
TGF-β3
2.8 
15,031 0.5
6.9 
8.2


Assay Performance Definitions
Assay working range – the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ)

Precision – the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy – ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) – the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal.

Bio-Plex® Technology and Applications


Bio-Plex Pro Rat Cytokine, Chemokine, and Growth Factor Assays are magnetic bead–based assays to detect and measure levels of 28 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell-signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth, to infections, to Parkinson's disease.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kits
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
  • Compatible with the new Bio-Plex Pro Rat Diabetes Assays for an expanded biomarker profile

Flexible Ordering Options

  • Premixed panels — complete ready-to-use kits (23-plex, 12-plex, TGF-β 3-plex)
  • Singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires a reagent kit (12002798 for magnetic separation) and a vial of standards (171NZ0001 for rat cytokines or 171X40001 for TGF-β assays) for a complete assay
  • Custom premixed assays — available as Bio-Plex® x-Plex™ Assays built using the Bio-Plex Assay Builder
  • Custom assays (mixed at the bench) —  available as Bio-Plex Express Assays built using the Bio-Plex Assay Builder

Instrument Compatibility

Compatible with these readers and wash stations:

  • Bio-Plex® MAGPIX™ or Luminex MAGPIX System
  • Bio-Plex 200 or Luminex 200 System
  • Bio-Plex 3D or Luminex FLEXMAP 3D System
  • Bio-Plex Pro™ Wash Stations

More Information

See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Bio-Plex Pro™ Rat Cytokine 23-Plex Assay

12005641
1 x 96-well, includes coupled beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, flat bottom plate, and sealing tape for detecting 23 rat cytokines all in a single well. This product replaces a discontinued assay (171K1001M)

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Bio-Plex Pro™ Rat Cytokine Th1/Th2 Assay

171K1002M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter and flat bottom plate, and sealing tape for the detection 12 rat cytokines all in one kit

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Bio-Plex Pro™ TGF-β 3-plex Assay

171W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, and sealing tape for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

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RUB79,275.00
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Number Description Options
10014905 Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays, Rev D Click to download
10000076327 Bio-Plex Pro™ Mouse and Rat Cytokine Quick Guide, Ver A Click to download
5297 Bio-Plex Pro™ Assay Reference Guide, Ver D Click to download
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Rev E Click to download [ Add to Cart (Free) ]
5405 Bio-Plex® Multiplex System Brochure, Ver E Click to download [ Add to Cart (Free) ]
6100 Bio-Plex Pro Rat Cytokine Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
6054 Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
2993 Bio-Plex Glossary, Rev A Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download