Bio-Gel P Polyacrylamide Gel

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Overview

Specifications
  Hydrated Bead Size (µm) Hydrated Bed Volume Mass Dry Gel Typical Flow Rate (cm/hr)* Molecular Weight Fractionation Range
Bio-Gel P–2, fine 45–90 3 ml/g 5–10 100–1,800
Bio-Gel P–2, extra fine <45   <10  
Bio-Gel P–4, medium 90–180 4 ml/g 15–20 800–4,000
Bio-Gel P–4, fine 45–90   10–15  
Bio-Gel P–4, extra fine <45   <10  
Bio-Gel P–6, medium 90–180 6.5 ml/g 15–20 1,000–6,000
Bio-Gel P–6, fine 45–90   10–15  
Bio-Gel P–6, extra fine <45   <10  
Bio-Gel P–6DG gel 90–180   15–20  
Bio-Gel P–10 gel, medium 90–180 7.5 ml/g 15–20 1,500–20,000
Bio-Gel P–10 gel, fine 45–90   10–15  
Bio-Gel P–30 gel, medium 90–180 9 ml/g 7–13 2,400–40,000
Bio-Gel P–30 gel, fine 45–90   6–11  
Bio-Gel P–60 gel, medium 90–180 11 ml/g 4–6 3,000–60,000
Bio-Gel P–60 gel, fine 45–90   3–5  
Bio-Gel P–100 gel, medium 90–180 12 ml/g 4–6 5,000–100,000
Bio-Gel P–100 gel, fine 45–90   3–5  
 

*Flow rates determined in a 1.5 x 70 cm column using hydrostatic pressure head-to-bed ratio of 1:1

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylenebisacrylamide. Bio-Gel P gels:

  • Are supplied dry and are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
  • Are extremely hydrophilic and essentially nonionic
  • Provide efficient, gentle gel filtration of sensitive compounds
  • Do not support microbial growth or leach carbohydrates (due to their synthetic composition), as dextrose and agarose gels can

Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C.

The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides, peptides, and tannins without altering the exclusion properties of the gels. Formamide may also be used at full strength since Bio-Gel P gels are completely swollen in this solvent.

Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilic nature ensures little or no interaction between the gel and sample molecules.

Bio-Gel P-2 Gel

150-4114
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range

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Bio-Gel P-2 Gel

150-4115
500 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range

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Bio-Gel P-2 Gel

150-4118
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 100–1,800 MW fractionation range

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Bio-Gel P-4 Gel

150-4120
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 800–4,000 MW fractionation range

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Bio-Gel P-4 Gel

150-4124
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 800–4,000 MW fractionation range

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Bio-Gel P-4 Gel

150-4128
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 800–4,000 MW fractionation range

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Bio-Gel P-6 Gel

150-4130
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-6 Gel

150-4134
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-6DG Gel

150-0738
100 g, polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-6DG Gel

150-0739
1 kg, fine polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-10 Gel

150-4140
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,500–20,000 MW fractionation range

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Bio-Gel P-10 Gel

150-4144
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,500–20,000 MW fractionation range

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Bio-Gel P-30 Gel

150-4150
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 2,500–40,000 MW fractionation range

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Bio-Gel P-30 Gel

150-4154
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 2,500–40,000 MW fractionation range

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Bio-Gel P-60 Gel

150-4160
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 3,000–60,000 MW fractionation range

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Bio-Gel P-60 Gel

150-4164
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 3,000–60,000 MW fractionation range

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Bio-Gel P-100 Gel

150-4170
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 5,000–100,000 MW fractionation range

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Bio-Gel P-100 Gel

150-4174
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 5,000–100,000 MW fractionation range

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Number Description Options
2068 Desalting With Bio-Gel P-6DG Desalting Gel, Rev B Click to download
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10006044 Instruction Manual, Profinia Purification and Buffer Kits, Rev D Click to download
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