Aurum™ Total RNA 96 Kit

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Overview

RNA Isolation Selection Guide
  Aurum™ Total RNA Kits PureZOL™ RNA Isolation Reagent
Mini Kit Fatty and Fibrous Tissue Kit 96 Kit
Format Mini column Filtration (vacuum or spin) Mini column Filtration (vacuum or spin) 96-well plate Filtration (vacuum or spin)
Maximum starting amounts        
   Cultured cells 2 x 106 1 x 106 1 x 107
   Bacterial cells 2.4 x 109 8 x 108 2.4 x 109
   Yeast cells 3 x 107 2 x 107 3 x 107
   Animal tissue — hard 20 mg 100 mg 100 mg
   Animal tissue — soft    to moderately hard 40 mg 100 mg 100 mg
   Plant tissue 40 mg 100 mg 100 mg
Isolation method Silica membrane Lysis with PureZOL Reagent, purification on silica membrane Silica membrane Organic extraction
Number of preps 10* or 50 mini preps 10* or 50 mini preps 2 x 96-well plate 50 or 100 preps (1 ml/prep)
Number of washes 3 3 3
DNase I included** Yes Yes Yes No
DNase I digest time 15 min (animal tissue, 25 min) 15 min 10 min
Total preparation time <50 min (1.5 hr with DNase I) <50 min (1.5 hr with DNase I) <60 min <60 min
Binding capacity >100 µg >100 µg >40 µg
Elution volume 2 x 40 µl 2 x 40 µl 80 µl 30–100 µl
Yield*** 28 µg/2 x 106 NIH 3T3 cells 96 µg/100 mg brain 10 µg/1 x 106 NIH 3T3 cells 1.15 µg/mg brain
  38 µg/2 x 106 HeLa cells 58 µg/100 mg breast 17 µg/1 x 106 HeLa cells 1.37 µg/mg fat
  20 µg/40 mg brain 85 µg/100 mg heart 11 µg/2 x 107 S. cerevisiae cells 1.2 µg/mg heart
  52 µg/20 mg thymus 63 µg/100 mg skin 5 µg/8 x 108 E. coli cells 0.66 µg/mg cartilage
  132 µg/40 mg spleen 54 µg/100 mg cartilage 5 µg/8 x 108 B. cereus cells 1.2 µg/mg skin
  36 µg/40 mg lung     1.0 µg/mg potato
  32 µg/40 mg potato     1.37 µg/1 x 105 293H cells
  28 µg/3 x 107 S. cerevisiae cells     0.1 µg/mg Arabidopsis
  54 µg/2.4 x 109 E. coli cells     0.38 µg/mg A. niger
        0.63 µg/2.4 x 107 E. coli cells
        0.31 µg/3 x 105 S. cerevisiae cells
Purity A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1
 

* Trial sizes of the Aurum Total RNA Mini Kit and Aurum Total RNA Fatty and Fibrous Tissue Kit are available.

** Removal not required.

*** Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.

The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.

Features include:

  • High-throughput total RNA isolation in less than 60 minutes
  • RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
  • Rapid silica binding-based purification technology in a 96-well plate format
  • Easy-to-use spin- or vacuum-compatible protocol
Format 96-well plate
Method Spin- or vacuum-mediated silica binding
Number of preps 192 (2 x 96-well plate)
Maximum starting material amounts 1 x 106 cultured cells
8 x 108 bacterial cells
2 x 107 yeast cells
Binding Capacity >40 µg purified total RNA
Purity A260/A280 of 1.9–2.1
RNA size distribution >200 nucleotides
Preparation time <60 min (including DNase I digest)
Maximum elution volume 80 µl
Minimum elution volume 30 µl*
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
Yield** 5 µg/8 x 108 E. Coli cells
5 µg/8 x 108 B. cereus cells
*The elution volume is reduced to 30µl when
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
 
**Yields will vary depending on the
developmental stage, tissue type or cell line,
and growth conditions used.
 
Aurum™ Total RNA 96 Kit

732-6800
DNA-free RNA isolation kit, includes RNA binding plates, collection microplates, lyophilized DNase I, RNase-free reagents, sealing tape, for 2 x 96-well preps

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Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
2919 Aurum Total RNA 96 Kit Product Information Sheet, Rev B Click to download
2925 Aurum Total RNA 96 Kit Protocol Overview Click to download
4110131 Instruction Manual, Aurum Total RNA 96 Kit, Rev C Click to download
6018 High-Throughput Automated Extraction of RNA Using the Aurum Total RNA 96 Kit, Rev A Click to download
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download [ Add to Cart (Free) ]