Your choice for ECL substrates is clear:
The Trans-Blot Turbo System enables fast, efficient, and reproducible transfer of proteins up to 400 kD. Preassembled Trans-Blot Turbo Transfer Packs are optimized for superior blot transfer.
This hydrophobic PVDF membrane is ideal for chemiluminescent and colorimetric western blots. High mechanical strength facilitates reprobing.
Bio-Rad’s Clarity family of Western ECL substrates are compatible with any HRP-conjugate secondary antibody and ideal for both digital and film-based imaging.
Clarity Max Substrate
The V3 Western Workflow streamlines the western blotting protocol, saving time and increasing accuracy and reliability by incorporating innovative tools throughout the process:
Bio-Rad carries a full line of gel and blot imaging systems, including its proprietary stain-free imaging technology.
Western Blot Doctor™ Troubleshooting GuideOur self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best.
Protein Blotting Guide(PDF 7.9 MB)Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.
Electrophoresis Guide(PDF 6.4 MB)A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more.
Western Blotting Techniques Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
Stain-Free Western Blot Imaging(VIDEO 11:41 mins)See the fast, convenient, and transparent V3 Western Workflow process, how Image Lab Software performs data normalization using total protein loading controls, and how stain-free imaging yields reliable western blot results.
Single-Cell Western Blot and Stain-Free Total Protein Loading Control(VIDEO 45:55 mins)Demonstration of quantitative analysis of fiber type–specific protein expression patterns in single muscle cells with a unique western blotting protocol utilizing Bio-Rad's stain-free imaging technology.
Protein Separation and AnalysisBio-Rad’s V3 Western Workflow™ facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
Can We Trust Western Blots? (VIDEO 45:41 mins)In this presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.
Protein ElectrophoresisFind protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.
Imaging and AnalysisFind information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software.
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