Your choice for ECL substrates is clear:
The Trans-Blot Turbo System enables fast, efficient, and reproducible transfer of proteins up to 400 kD. Preassembled Trans-Blot Turbo Transfer Packs are optimized for superior blot transfer.
This hydrophobic PVDF membrane is ideal for chemiluminescent and colorimetric western blots. High mechanical strength facilitates reprobing.
Fluorescent western blot antibodies provide unmatched sensitivity and easy multiplexing compared to conventional western blotting methods.
Browse all fluorescent antibodies for western blotting
The V3 Western Workflow streamlines the western blotting protocol, saving time and increasing accuracy and reliability by incorporating innovative tools throughout the process:
Bio-Rad carries a full line of gel and blot imaging systems, including its proprietary stain-free imaging technology.
(Note: This system is not enabled for stain-free detection)
Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best.
(PDF 7.9 MB)Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.
(PDF 6.4 MB)A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more.
Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
Bio-Rad's V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
(VIDEO 11:41 mins)See the fast, convenient, and transparent V3 Western Workflow process, how Image Lab Software performs data normalization using total protein loading controls, and how stain-free imaging yields reliable western blot results.
(VIDEO 45:55 mins)Demonstration of quantitative analysis of fiber type–specific protein expression patterns in single muscle cells with a unique western blotting protocol utilizing Bio-Rad's stain-free imaging technology.
(VIDEO 45:41 mins)In this presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.
Find protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.
Find information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software.
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