These rigid macroporous hydrophilic media meet the demands of process-scale applications. Chemically and mechanically stable, the media operate well at low and medium pressures.
* Determined with human IgG.
Effect of flow rate on separation and peak symmetry. A 5 ml sample (1.4 mg/ml) of myoglobin (peak 1), ribonuclease A (peak 2), and cytochrome c (peak 3) was run on a 1 x 13 cm (8.7 ml) column at each of the indicated flow rates. The buffer was 20 mM potassium phosphate, pH 8.0, with a gradient of 0–1M KCl over 75 ml.
To read more click here
Clearance of Murine Leukemia Virus (MuLV) from a chimeric Monoclonal Antibody Using Ion exchange Chromatography
Virus validation with a model virus (or viruses) demonstrates to what extent a purification scheme is capable of reducing and/or removing viral particles that may be present in a cell line producing a biologic. Demonstrating potential virus inactivation and/or removal via the production process is recommended in the production of biologicals (US FDA 1993). The number and type of viruses used in a validation study depends upon many factors, including the type of cell line the product is from, and the intended use of the product (US FDA 1993). Virus validation is usually performed after a purification scheme has been developed and scaled up, so it is important to consider matrices that remove contaminating viruses (and nucleic acids in general) from the feed stream during the development of the purification. The ramifications of the chosen chromatographic steps should be evaluated for the positive implications on the virus validation study.
To read more click here
Customer Services & Ordering: +7 (495) 721 14 04
Sales: +7 (495) 721 14 04
Product Support: +7 (495) 721 14 04
Instrument Service: +7 (495) 721 14 04
Please reenter your email address in the correct format.
Please enter your email address.
Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.
To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..
Terms & Conditions
Copyright © 2015 Bio-Rad Laboratories, Inc. All rights reserved.