Droplet Digital PCR (ddPCR™) is Bio-Rad's unique digital PCR technology. With unrivaled precision, ddPCR provides absolute quantification of target DNA or RNA molecules without the use of standard curves. ddPCR addresses the lack of scalable and practical technologies for digital PCR implementation. The QX200™ ddPCR System puts this powerful technology in your hands, ready to unveil new worlds of research at previously unattainable levels.
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments.
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Genomic Variation Analysis: Single Nucleotide Polymorphism: Validation of Allele-Specific Expression Predicted by RNA-Seq in Human Brain SpecimensIn the context of human brain development, the QX100™ Droplet Digital™ PCR System yields unambiguous results for genotype identification of low-expression genes and provides absolute quantification of allele-specific expression (ASE) without the use of a standard.Download the article
Genomic Variation Analysis: Copy Number Variation: DUF1220 Copy Number Variation across Healthy and Diseased PopulationsStudying copy number variation (CNV) with the QX100 Droplet Digital PCR System allows the robust identification of genetic factors such as DUF1220 in neurogenetic diseases between individuals with greater accuracy and precision, while effectively validating array CGH data. Download the article
Genomic Variation Analysis: Single Nucleotide Polymorphism: Detection of Single Nucleotide Variation Frequencies by Droplet Digital™ PCRThe QX100 Droplet Digital PCR System validated DNA sequencing results of induced pluripotent stem cells (iPSCs). In addition to confirming the high frequency of single nucleotide variations (SNVs), a de novo type of SNV was identified.Download the article
Cancer Mutation Analysis: How Droplet Digital PCR Has Revolutionized Noninvasive Plasma-Based Detection of MutationsPerforming Droplet Digital PCR with the QX100 Instrument effectively detected and accurately quantified the EGFR T790M marker in targeted therapies with limited cancer tumor samples. Download the article
Viral Quantification: Adeno-Associated Virus Vector Genome Titer AssayDetermining the adeno-associated virus (AAV) vector genome (vg) titer assay is critical in gene therapy applications. The improved sensitivity observed with Droplet Digital PCR (ddPCR™) technology makes it highly suitable for titration of clinical-grade AAV vector preparations.Download the article
Gene Expression: Digital Quantification of Potential Therapeutic Target RNAsMeasuring the exact number of long noncoding RNAs (lncRNAs) in subcellular compartments with Droplet Digital PCR (ddPCR) provides insights into their role and mechanism of action.Download the article
Third Generation PCR: A Novel Way to Accurately Measure Copy Number Variations in Human GenomeThe McCarroll Lab uses Droplet Digital technology to study the distribution and molecular properties of the genome and its influence on gene expression, as well as the genetics underlying schizophrenia and bipolar disorder.Download the article
Success Video: QX100™ Droplet Digital™ (ddPCR™) System at Sangamo BioSciences
Droplet Digital™ PCR opens new perspectives in HIV research. Sangamo BioSciences, Inc. is developing HIV therapeutics toward a functional cure using the company’s proprietary zinc finger DNA-binding protein technology.Download the article
Using Droplet Digital™ PCR to Diagnose Leukemia
Droplet Digital PCR in theScientific Literature
ddPCR in Cancer Research,Diagnostics, and Genotyping
Simple and reliable, Droplet Digital PCR technology has already led to several research breakthroughs in fields such as cancer biomarker discovery, infectious diseases, genomic alterations, and gene expression. The benefits of ddPCR are:
Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. PrimePCR™ ddPCR Assays are now available for mutation and copy number detection. These are predesigned, wet-lab validated assays.
Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.
Perform accurate quantification and qualification of NGS libraries without the use of a standard curve using the Digital PCR Library Quantification Kits.
Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.
Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.
Test a wide variety of environmental samples like soil and water using the QX200 System.
See more Digital PCR applications.
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