Automated electrophoresis systems integrate the process of separation, detection, and data analysis of proteins or nucleic acids in a single platform and remove possible manual errors during the multifarious steps of the electrophoresis process. Using much smaller sample and reagent quantities and less hands-on time than standard analysis methods, automated electrophoresis systems like Bio-Rad's Experion™ automated electrophoresis system can be used in applications such as purity and yield assessment in protein purification and protocol optimization workflows, RNA integrity assessments before RT-qPCR or next generation sequencing, or separation and detection of DNA fragments from PCR reactions or RFLP analysis.
This section describes the Experion automated electrophoresis system from Bio-Rad and how it can be used in the automated electrophoresis workflow. To learn more about the technology behind the Experion system, visit our Experion automated electrophoresis system section.
Related Topics: Protein Analysis Using the Experion System and Nucleic Acid Analysis Using the Experion System.
The Experion automated electrophoresis system employs LabChip microfluidic technology to automate protein and nucleic acid electrophoresis. Using much smaller sample and reagent quantities than standard analysis methods, the Experion system accomplishes analysis in a single 30–40 minute, automated step.
The Experion system includes: 1) automated electrophoresis station, 2) priming station, 3) vortex station used for nucleic acid analysis only, 4) system operation and data analysis tools (software), and 5) analysis kits, which include the (a) chips and (b) reagents for protein (Pro260 kit), standard-sensitivity RNA (StdSens kit), high-sensitivity RNA (HighSens kit), and DNA (DNA 1K and 12K kits) analyses.
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The Experion system is used for the following applications:
Sample analysis involves the following steps:
Experion software display.
Protein, RNA, or DNA analysis with the Experion system is achieved with various analysis kits, which supply the microfluidic chips, reagents, and instructions required to separate and analyze 10–260 kD proteins (under denaturing conditions), total or mRNA, and double-stranded DNA.
The Experion system can be used for protein applications such as:
See Protein Analysis Using the Experion System for more information.
The Experion system can be used for nucleic applications such as:
See Nucleic Acid Analysis Using the Experion System for more information.
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