Detergents for Protein Sample Preparation

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Overview

SDS Solutions
Solution Formulation Applications
10% SDS solution 10% (w/v) sodium dodecyl sulfate SDS-PAGE; for preparing sample gel and running buffers
20% SDS solution 20% (w/v) sodium dodecyl sulfate Northern and Southern hybridization buffer component

Detergents are used in electrophoresis when it is necessary to disrupt protein-lipid or protein-protein interactions. SDS is the most common detergent used in PAGE analysis, because most proteins are readily soluble in it. Bio-Rad SDS solutions are highly purified — an important feature, since impurities in SDS have unpredictable effects on electrophoretic mobility. SDS solutions provide highly purified SDS in convenient 10% and 20% solutions. They are prepared with 18 MΩ water and have no detectable DNase or RNase activity. Highly purified SDS powder gives excellent results in the electrophoresis method of Laemmli (1970) and optimizes activity recovery in enzyme purification applications.

Tween 20 for blotting, and Triton X-100 and CHAPS for membrane protein solubilization are also available. For simple, accurate pipetting, a solution of 10% Tween 20 is also available.

 

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SDS (Sodium Dodecyl Sulfate)

1610301
100 g, sodium dodecyl sulfate (SDS) powder

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¥12,000
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SDS (Sodium Dodecyl Sulfate)

1610302
1 kg, sodium dodecyl sulfate (SDS) powder

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¥96,000
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SDS Solution

1610416
250 ml, 10% (w/v) sodium dodecyl sulfate (SDS) solution

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¥14,500
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SDS Solution

1610418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution

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¥24,000
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Triton X-100 Detergent

1610407
500 ml, Triton X-100 detergent

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¥9,000
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CHAPS

1610460
1 g, CHAPS detergent powder

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¥14,000
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Tween 20

1706531
100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)

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¥8,500
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10% Tween 20

1610781
1 L, detergent, for easy pipetting

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¥8,500
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2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]