Nuclear Staining Dyes
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PureBlu™ Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for flow cytometry, fluorescence microscopy and cell imaging applications.
Based on the well-recognized DAPI and Hoechst 33342 chemistries, PureBlu Dyes are offered in a ready-to-reconstitute, high-purity powder format.
PureBlu Nuclear Staining Dye One-Step Dilution
PureBlu DAPI and PureBlu Hoechst 33342 Dyes are both ready to use after a single dilution — no weighing required.
Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to 50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM); PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)). Note: the optimal concentration for different cell types and applications should be determined empirically.
Features and Benefits of PureBlu Nuclear Staining Dyes
- High purity formulation (>95%) for reliable results in challenging experiments
- Pre-aliquoted powder format eliminates time-consuming weighing steps
- Convenient format allows you to generate working solution with only one dilution step after resuspension
- Compatible with multicolor experiments
Applications and Uses of PureBlu Nuclear Staining Dyes
- Nuclear staining
- Microscopy and cell imaging
- Multicolor experiments
- Cell Cycle analysis
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DAPI can be used for cell viability analysis in flow cytometry
Ordering Information
| Catalog Number |
PureBlu™ Nuclear Staining Dye |
Excitation Maximum, nm |
Emission Maximum, nm |
Optimal Excitation Laser, nm | S3e Cell Sorter | ZE5 Cell Analyzer | ZOE Fluorescent Cell Imager |
|---|---|---|---|---|---|---|---|
| 135-1303 | DAPI* | 359 | 461 | 355 |
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| 135-1304 | Hoechst 33342** | 350 | 461 | 355 |
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* Includes 5x50 µg vials of DAPI powder.
** Includes 5x56 µg vials of Hoechst 33342 powder.