MUD and MUG Microplates

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MUD and MUG Microplates


MUD and MUG microplates were developed to streamline and miniaturize testing for fecal contamination using a microplate format. These microplates are used to detect and enumerate two indicators of the presence of fecal material, E. coli and Enterococci, in diverse water sources.

Intended Use

Water-related health hazards are a worldwide problem. Contamination of water by fecal matter is common. MUD and MUG microplates were developed to test for E. coli and Enterococci in all types of surface and waste water, particularly those rich in suspended matter, such as bathing, fresh, and waste water. MUG plates can also be used for the quantitative detection of E. coli in sludge, soil, soil improvers, growing media, and bio-waste.

Key Benefits

  • MUG/EC microplates are used to detect E. coli, while MUD/SF plates are used to detect Enterococci
  • Method requires no growth in broth or on agar plates
  • Microplate format permits dilutions and replicates of a sample on the same plate
  • Diluents available include one formulation containing bromophenol blue for easy visualization
  • Simple and economical detection with long wavelength UV lamps
  • Use with Fluoplate™2 Software (3591790) for analysis and full traceability reports

Principle

The method is based on detection of the two different enzymes, one present in most E. coli, the other in most Enterococci. The substrate MUG (4-methylumbelliferyl-β-D-glucuronide) is hydrolyzed by β-D-glucuronidase, an enzyme present in E. coli, while the enzyme β-D-glucosidase present in Enterococci hydrolyzes the substrate MUD (4-methylumbelliferyl-β-D-glucoside or 4-methylumbelliferyl-β-D-glucopyranoside). Hydrolysis of both MUG and MUD yields 4-methylumbelliferone, which fluoresces blue with λex 365 nm and λem 445 nm. Water samples (dilution series depends on the source) are added to microplates containing either dehydrated MUD or MUG. After incubation, for 36 to 72 hours at 44°C, wells are read with a UV reader to score the number of wells with fluorescence.

Fluoplate2 Software provides intuitive, simple data handling, analysis, and reports. MUD and MUG microplate analysis uses Most Probable Number (MPN) estimation and its confidence intervals, based on the Poisson distribution for rare events. The Fluoplate2 statistical analysis software provides immediate MPN estimation of E. coli and Enterococci. (If the software is not being used, the statistical tables for MPN determination of water samples are available on request. Please contact your local sales representative.)

Validations

MUD and MUG plates are standardized through EN ISO 9308-3 (1999) and EN ISO 7899-1 (1999). MUG microplates are recommended for the quantitative detection of E. coli in sludge, soil, soil improvers, growing media, and bio-waste in European Technical Report FD CENT/TR 15214-2 in April 2006.

Product Options

  • MUG/EC Microplates for E. coli Testing, opaque (3553785) or transparent (3553782)
  • MUD/SF Microplates for Enterococcus Testing, opaque (3553786) or transparent (3553783)
  • Diluents available, Sterile Distilled Water (EDS) (3554283), DSM (contains synthetic sea salt), dehydrated (3553784) or with Bromophenol Blue, ready to use (3554282)
  • Fluoplate2 Software (3591790)

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

MUG/EC Microplates for E. coli Testing, opaque

3553785
Pkg of 25, 96-well opaque microplates, 4-methylumbelliferyl-beta-d-glucuronide (MUG) media for E.coli testing

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€300.00
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MUG/EC Microplates for E. coli Testing, transparent

3553782
Pkg of 25, 96-well clear microplates, 4-methylumbelliferyl-beta-d-glucuronide (MUG) media for E.coli testing

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€300.00
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MUD/SF Microplates for Enterococcus Testing, opaque

3553786
Pkg of 25, 96-well opaque microplates, 4-methylumbelliferyl-beta-d-glucoside (MUD) media for Enterococcus testing

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€300.00
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MUD/SF Microplates for Enterococcus Testing, transparent

3553783
Pkg of 25, 96-well clear microplates, 4-methylumbelliferyl-beta-d-glucoside (MUD) media for Enterococcus testing

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€300.00
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DSM (Special Microplate Diluent) with Bromophenol Blue

3554282
Pkg of 60, 18 ml tubes, diluent containing synthetic sea salt for use with MUD and MUG Microplates, contains 4 µg/ml Bromophenol Blue

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€126.00
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DSM (Special Microplate Diluent), dehydrated

3553784
Pkg of 1, 100 g of dehydrated diluent containing synthetic sea salt, for use with MUD and MUG Microplates, makes 4.44 L

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€44.00
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Sterile Distilled Water (EDS) for Microplates

3554283
Pkg of 60, 18 ml tubes, sterile distilled water for use with MUD and MUG Microplates

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€129.00
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Square Sterile Petri Dishes, 120 mm

3550304
Pkg of 252, presterilized square plastic Petri plates, 120 mm

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€155.00
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FSD_17932 Water Safety Booklet - Guide for Products in Water Testing Click to download