SsoAdvanced™ Universal SYBR® Green Supermix is an exclusive high-performance real-time PCR reagent based on Bio-Rad's patented* Sso7d fusion protein polymerase technology and advanced buffer formulation.
This supermix is uniquely formulated to provide higher processivity, increased PCR inhibitor tolerance, and robust performance with challenging templates and targets in SYBR® Green-based qPCR. It delivers superior performance for a wide range of applications on any qPCR platform and is validated to ensure optimal results with Bio-Rad’s PrimePCR™ Gene Expression Assays.
- Achieve superior real-time PCR results under various experimental conditions — for example, primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)
- Increase your qPCR sensitivity and efficiency of detection from compromised samples — the highly processive Sso7d fusion polymerase has increased resistance to various PCR inhibitors, enhancing sensitivity and overall performance
- Shorten time to results while maintaining qPCR data quality — the Sso7d fusion polymerase and optimized buffer provide rapid polymerization kinetics and instant polymerase activation
- Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
- 7500, ViiA 7
- 7000, 7300, 7700, 7900HT, QuantStudio Systems
- Mastercycler ep realplex 2, 4
- Corbett Rotor-Gene 3000, 6000, Q
- LightCycler 480, 96
- LightCycler 1.0, 1.5, 2.0*
- LightScanner HR-1
- LightScanner 32*
* BSA must be added according to instrument specifications
Bio-Rad offers complete workflow solutions for real-time PCR:
To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.