SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions #1725085

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Overview

Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

Safety Data Sheet

Safety Data Sheet

Description

Description

Use the SingleShot™ SYBR® Green Kit to obtain high-quality RT-qPCR data with SYBR® Green chemistry directly from cell culture lysates.

Two-step workflow provides results less than 2 hours after cell lysis. All RT-qPCR reagents and RNA control included.

Benefits include:

Optimized Sample Input

The SingleShot™ SYBR® Green Kit maximizes cell lysate and cDNA inputs in a two-step RT-qPCR reaction:

Maximum cell lysate into RT reaction 45% of total volume
Maximum undiluted cDNA into qPCR reaction 20% of total volume

 

Kit Contents

 

SingleShot Cell Lysis Reagents
SingleShot Cell Lysis Buffer
SingleShot Proteinase K Solution
SingleShot DNase Solution

100 x 50 μl reactions
5 ml (1 x 5 ml vial)
100 μl (1 x 1 ml vial)
100 μl (1 x 1 ml vial)

cDNA Synthesis Kit
5x iScript Advanced Reaction Mix
iScript Advanced Reverse Transcriptase
Nuclease-Free Water

100 reactions
400 μl (1 x 1 ml vial)
100 μl (1 x 1 ml vial)
1 ml (1 x 1 ml vial)

qPCR Supermix
SsoAdvanced Universal SYBR® Green Supermix

500 reactions
5 ml (5 x 1 ml vials)

SingleShot RNA Control
SingleShot RNA Control Template
SingleShot™ SYBR® Green qPCR Control Assay

200 reactions
Lyophilized (200 reactions)
200 μl (1 x 1 ml vial)

Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot™ SYBR® Green Kit

The SingleShot™ SYBR® Green Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line Property Species
A549 Adherent  H. sapiens, lung carcinoma
HeLa Adherent H. sapiens, cervical adenocarcinoma
HepG2   Adherent  H. sapiens, liver carcinoma
NIH/3T3 Adherent M. musculus, embryonic fibroblast
MCF-7 Adherent  H. sapiens, epithelial, adenocarcinoma
NCI-H1975 Adherent H. sapiens, lung adenocarcinoma 
K-562 Suspension H. sapiens, chronic myelogenous leukemia
HEK 293 Suspension H. sapiens, kidney
Jurkat Suspension H. sapiens, T lymphocyte
PC-12  Suspension R. norvegicus, pheochromocytoma, adrenal
HepRG   Primary-like H. sapiens, hepatocytes

 

Related Products

The SingleShot™ SYBR® Green Kit belongs to the family of SingleShot™ Cell Lysis RT-qPCR Kits:

  • SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit (172-5095) and SingleShot Probes One-Step Kit (172-5070)
  • SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit (172-5085) and SingleShot Probes Kit (172-5090)
  • SingleShot Cell Lysis Kit — available for 100 reactions (172-5080)

Specifications

Specifications

Storage at –20°C
12 months:
Lysis buffer, DNase, and proteinase K solution
iScript Advanced cDNA Synthesis Kit
SsoAdvanced™ Universal SYBR® Green Supermix
SingleShot RNA Control Template
SingleShot™ SYBR® Green qPCR Control Assay
Storage at 4°C
6 months:
Lysis buffer

Up to 3 months:
SsoAdvanced™ Universal SYBR® Green Supermix