For the detection of red cell antigens A1 and H Seraclone™ Anti-A1 and Seraclone™ Anti-H are used in ABO blood group typing to differentiate A1 and A2 red cells.
Seraclone™ Anti-A1 and Seraclone™ Anti-H contain monoclonal antibodies of immunoglobulin class IgM as their reactive component. They are obtained from the cell culture supernatant of hybridoma cell lines derived from the fusion of antibody-producing murine B lymphocytes with murine myeloma cells. They display the constant specificity and reproducibility typical of monoclonal antibodies.
Seraclone™ Anti-A1 Clone A1
Seraclone™ Anti-H Clones A46/B.B10
- The monoclonal antibodies in Seraclone™ Anti-A1 and
- Seraclone™ Anti-H agglutinate in a saline milieu and are reactive at room temperature.
- Seraclone™ Anti-A1 and Seraclone™ Anti-H are used in tube tests. Seraclone™ Anti-H reacts with cells carrying the H substance e.g. A2, A3, Ax, weak A variants and null erythrocytes. It is therefore used to differentiate A1 and A2 erythrocytes. As H receptors are also present in small quantities on B and A1 erythrocytes, Seraclone™ Anti-H can have a weak positive reaction with B and in rare cases with A1 erythrocytes. With A2B cells Seraclone™ Anti-H reacts negatively in most cases. Seraclone™ Anti-A1 may have a weak positive reaction with A2 erythrocytes.