RC DC™ Protein Assay Kit I #5000121

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<em>RC DC</em>™ Protein Assay Kit I

Colorimetric assay kit, includes RC Reagents Package, DC™ Protein Assay Reagents Package, bovine γ-globulin standard; 450 standard assays

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500-0121-MSDS DC Universal Protein Assay Kit I English
French

Use the RC DC Protein Assay for samples containing reducing agents and detergents. This assay, based on the DC Protein Assay, is a modified form of the Lowry assay that is both reducing agent compatible (RC) and detergent compatible (DC), whereas the DC Assay is only detergent compatible.

  • Linear over a wide range of protein concentration; standard assay, 0.125–1.5 mg/ml and low concentration assay, 5–250 µg/ml
  • Read wavelengths, 650–750 nm
  • Absorbance values are stable ≥ 1 h
  • Quantitate protein in sample loading buffer prior to gel electrophoresis, either 1-D or 2-D format
  • Determine protein recovery in cell lysates

Sample components compatible with the RC DC Protein Assay include:

  • Ammonium sulfate, 0.5 M
  • β-mercaptoethanol, 10%*
  • Brij 35, 1%
  • C12E8, 0.2%
  • Calcium chloride, 50 mM
  • CHAPS, 2%
  • CHAPSO 1%
  • Dithiothreitol (DTT), 350 mM*
  • EDTA 0.1 M
  • Guanidine hydrochloride, 0.4 M
  • Imidazole, 0.5 M
  • Laemmli buffer (5% β-mercaptoethanol)
  • Nonidet P-40, 2%
  • Octyl-β-glucoside, 1%
  • ReadyPrep™ Sequential Extraction Reagents 2 and 3*
  • SDS, 10%
  • Tributylphosphine (TBP), 2 mM
  • Tris, pH 8.4, 0.5 M
  • Triton X-100, 2%
  • Tween 20, 2%
  • Urea, 4 M


*Two washes required for highest concentrations

Product Contents

  • RC Reagent I, 250 ml
  • RC Reagent II, 250 ml
  • Alkaline copper tartrate, 250 ml
  • Dilute Folin reagent, 2 L
  • Surfactant solution, 5 ml
  • Lyophilized bovine γ-globulin, 1 vial

Packaging Options

Kit Protein Standard Catalog Number
RC DC Protein Assay Kit I Bovine γ-globulin 5000121
RC DC Protein Assay Kit II Bovine serum albumin 5000122

Related Products

  • Protein Standard I, lyophilized bovine γ-globulin (5000005)
  • Protein Standard II, lyophilized bovine serum albumin (5000007)
  • Semimicrovolume Disposable Polystyrene Cuvettes (2239955)
  • Standard Disposable Polystyrene Cuvettes (2239950)
  • Protein Assay Reagent A, refill, 250 ml alkaline copper tartrate (5000113)
  • Protein Assay Reagent B, refill, 1 L Folin reagent (5000114)
  • Protein Assay Reagent S, refill, 5 ml surfactant (5000115)
  • RC Reagent I, refill, 250 ml (5000117)
  • RC Reagent II, refill, 250 ml (5000118)

Related Categories

Supporting Documentation

Reference

Lowry OH et al. (1951). Protein Measurement with the Folin Phenol Reagent. J Biol Chem 193, 265-275. PMID: 14907713