CFX96™ Touch Deep Well™ Real-Time PCR Detection System #1854095

CFX96™ Touch Deep Well™ Real-Time PCR Detection System

Modular thermal cycler platform, includes C1000 Touch™ Thermal Cycler Cchassis, CFX96™ Deep Well Optical Reaction Module, CFX Manager™ Software, qbase+PLUS Software license, cables

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Thermal Cycler  
Chassis C1000 Touch™
Maximum ramp rate, °C/sec 2.5
Average ramp rate, °C/sec 2
Heating and cooling method Peltier
Lid, °C Heats up to 105
Range, °C 0–100
Accuracy, °C ±0.2 of programmed target at 90°C
Uniformity, °C ±0.4 well-to-well within 10 sec of arrival at 90°C
Operational range, °C 30–100
Programmable span, °C 1–24
Optical Detection  
Excitation 6 filtered LEDs
Detection 6 filtered photodiodes
Range of excitation/emission wavelengths, nm 450–730
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Scan Time  
All channels, sec 12
FAM/SYBR® Green only, sec 3
Operating systems Windows XP, Windows 7
Multiplex analysis Up to 5 targets per well
Licensed for real-time PCR Yes
Sample capacity, wells 96
Sample size, µl 10–125
Communication interface USB 2.0
Electrical approvals IEC, CE
Dimensions (W x D x H), cm/in 33 x 46 x 36/13 x 18 x 14
Weight, kg/lb 21/47

Use the CFX96 Deep Well Real-Time PCR Detection System for precise, reproducible real-time PCR. The system is composed of the CFX96 Deep Well Optical Reaction Module and the C1000 Touch Thermal Cycler Chassis. The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Chassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.

Features and Benefits

  • Deep wells for large volumes
  • Detection of up to 5 targets per well, plus a channel dedicated to singleplex FRET
  • Protocol autowriter that generates an optimal protocol for your reaction components
  • Thermal gradient that identifies optimal annealing temperature in a single run
  • CFX Manager™ Software and qbase+ Software for reliable validation, data analysis, and export of RDML files for conformance with MIQE guidelines

The CFX96 Deep Well Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).

The optical system, with six filtered LEDs and six filtered photodiodes, collects data from all wells, detecting up to five targets per well. For single-color FAM and SYBR® Green I, the fast scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.

The large touch-screen interface has intuitive graphical programming and displays data traces in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.

Product Components

  • C1000 Touch Thermal Cycler Chassis (1841100)
  • CFX96 Deep Well Optical Reaction Module (1840197)
  • USB cable
  • CFX Manager Software installation CD
  • Instruction manual
  • qbase+ Software license
  • CFX Manager Software quick guides for system installation, protocol, plate, data analysis, gene expression analysis, and qbase+ Software setup
  • CFX Manager Software video tutorial

Configuration Options

The following modules can be used with the C1000 Touch Thermal Cycler Chassis:

Reaction Modules Catalog Number
  Module Module with Starter Package*
For PCR    
Dual 48/48 Fast Reaction Module 1840148  
96-Well Fast Reacton Module
96–Deep Well Reaction Module 1840197  
384-Well Reaction Module 1840138  
For Real-Time PCR    
CFX96 Optical Reaction Module 1845097 1845096
CFX96 Deep Well Optical Reaction Module 1844095 1844096
CFX384™ Optical Reaction Module 1845385 1845384

*Starter package contains SsoAdvanced™ Universal SYBR® Green Supermix (1725270), Microseal® 'B' Adhesive Seals (MSB1001), and Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates (HSP9601) or Hard-Shell Thin-Wall 384-Well Skirted PCR Plates (HSP3805).

The CFX96 Deep Well Real-Time PCR Detection System can be configured with other PCR instruments, allowing stand-alone or software control. Instrument permutations allow up to four different CFX Connect™, CFX96, or CFX384 Systems to be controlled by one PC.

Combine any two CFX Real-Time PCR Detection Systems with the CFX Automation System II, our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.

CFX Manager Software Works Seamlessly with PrimePCR™ Assays and Panels

Bio-Rad offers 96- and 384-well PrimePCR Assay Plates that contain primer pairs lyophilized in the plate wells. You may customize the genes on the plates or choose from a wide variety of predesigned disease- and pathway-specific panels. CFX Manager Software run files can be downloaded for all PrimePCR Assay plates, both predesigned and custom, providing complete gene layout and cycling protocol information for all your assays.
  Available in the U.S., Canada, Australia, New Zealand, Singapore, and E.U. only.