Bio-Plex Pro™ Rat Cytokine Th1/Th2 Assay #171K1002M

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Overview

1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter and flat bottom plate, and sealing tape for the detection 12 rat cytokines all in one kit
Safety Data Sheet

Safety Data Sheet

Description

Description

The Bio-Plex Pro Rat Cytokine Th1/Th2 12-Plex Immunoassay is a 1 x 96-well kit that includes coupled magnetic beads, detection antibodies, standards, assay buffer, wash buffer, sample diluent, standard diluent, detection antibody diluent, streptavidin-PE, a flat bottom plate, and sealing tape. This multiplex assay detects:

  • GM-CSF
  • IFN-γ
  • IL-1α
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-12 (p70)
  • IL-13
  • TNF-α

Based on 6.5 µm magnetic beads, it is compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic beads for simplified plate processing
  • All-in-one, space-saving complete kit
  • Premixed standards for generation of curves over a broad dynamic range for each target

Other Assay Formats Available

  • Custom premixed assays: available as Bio-Plex® x-Plex™ Assays
  • Custom assays (mixed at the bench): available as Bio-Plex Express Assays
  • Singleplex sets (each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires a reagent kit for magnetic separation (12002798) and a vial of standards (171NZ0001) for a complete assay)

For multiplexing capabilities with other cytokines, refer to Bulletin 5927. For multiplexing capabilities with diabetes assays, refer to Bulletin 5975.

Instrument Compatibility

  • Bio-Plex® MAGPIX™ or Luminex MAGPIX System
  • Bio-Plex 200 or Luminex 200 System
  • Bio-Plex 3D or Luminex FLEXMAP 3D System
  • Bio-Plex Pro Wash Stations

More Information

To learn more about performance characteristics or how to set up your rat cytokine, chemokine, and growth factor assays for optimal performance and sensitivity, see our supporting documents:

Other Available Rat Cytokine, Chemokine, and Growth Factor Assays

Browse cytokines, chemokines, and growth factors from other assay panels — we recommend combining singleplex sets only when they are from the same panel.