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Description
Description
Bio-Rad's Stain-Free Western Blotting Workflow is a five-step approach to streamlining your western blotting protocol. The V3 Western Workflow combines traditional blotting techniques with innovative tools that quickly allow checking electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection to obtain results faster.
Benefits
- Speed — rapid, high-quality separation of proteins achieved in as little as 20 minutes and efficient protein transfer completed in as little as 7 minutes
- Confidence — confirmation of protein separation and transfer without staining and destaining provides checkpoints throughout a western blotting protocol
- Quantitation — quantitation and normalization made simple with stain-free technology
The V3 Western Workflow complete system for midi gels includes the following:
- 50 Criterion TGX Stain-Free 4–20% 18-well precast gels (567-8094)
- 1 Criterion cell (165-6001)
- 5 Tris/glycine/SDS running buffer, 10x (161-0732)
- 1 Laemmli sample buffer (161-0737)
- 1 Precision Plus Protein WesternC standard pack — includes Strep-Tactin conjugate (161-0385)
- 1 Precision Plus Protein All Blue standard (161-0373)
- 1 Precision Plus Protein Unstained standard (161-0363)
- 1 Trans-Blot Turbo transfer system (170-4155)
- 5 Trans-Blot Turbo midi PVDF transfer packs, 10 per pack (170-4157)
- 5 Immun-Blot low fluorescence midi PVDF filter paper sets, 10 per pack (162-0262)
- 1 ChemiDoc MP imaging system (170-8280)
- 1 ChemiDoc MP red LED module kit (170-8283)
- 1 ChemiDoc MP green LED module kit (170-8284)
- 1 ChemiDoc MP blue LED module Kit (170-8285)
- 1 Clarity western ECL substrate, 200 ml (170-5060)